“…This analysis included the relative quantification of 1,263 proteins, the most downregulated of which were structural subunits of cIII and cI (Figs 2A and EV1). These included CHCHD2, whose knock-down causes cIV deficiency (Baughman et al, 2009;Imai et al, 2019), HIGD2A, one of the human orthologs of yeast Rcf1, which stabilizes the interaction between cIII 2 and cIV (Chen et al, 2012;Strogolova et al, 2012;Vukotic et al, 2012;Rieger et al, 2017), and GHITM or growth hormone-inducible transmembrane protein, a member of the BAX inhibitor motif-containing (TMBIM) family. Conversely, the tenth cI assembly factor detected in this analysis, NDUFAF2 (Ogilvie et al, 2005), and the cII assembly factor SDHAF2 (Hao et al, 2009) were significantly more abundant in D4-CYB mitochondria.…”