Lifestyle mediates the role of nutrient-sensing pathways in cognitive aging: cellular and epidemiological evidence

Lucia, Chiara de; Murphy, Tytus; Steves, Claire J.; Dobson, Richard; Proitsi, Petroula; Thuret, Sandrine

doi:10.1038/s42003-020-0844-1

Cited by 31 publications

(42 citation statements)

References 92 publications

(109 reference statements)

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“…The human fetal hippocampal multipotent progenitor cell line HPC0A07/03 (HPC; ReNeuron Ltd., Surrey, United Kingdom) was used in all experiments as previously described ( de Lucia et al, 2020 ; Smeeth et al, in press ). Cells were acquired from 12-week old female fetal tissue in accordance with United States and United Kingdom ethical and legal guidelines.…”

Section: Methodsmentioning

confidence: 99%

“…The HPC proliferation assay was carried out as previously described ( de Lucia et al, 2020 ; Smeeth et al, in press ). Briefly, HPCs were seeded into two 96-well plates (Nunclon, Denmark) per experiment: one plate for acute (one-time) caffeine treatment, the other for repeated caffeine treatment.…”

Section: Methodsmentioning

confidence: 99%

“…Control conditions were incubated in caffeine-free proliferation media in all instances. Seventy-two hours after seeding, all plates were washed and fixed as previously described ( de Lucia et al, 2020 ; Smeeth et al, in press ). Figure 1 depicts the assay timeline as per the two exposure conditions.…”

Section: Methodsmentioning

confidence: 99%

“…Cell count, progenitor cell integrity, progenitor proliferation, and cell death were visualized using 4′,6-diamidino-2-phenylindole (DAPI), Nestin and SRY-Box Transcription Factor 2 (SOX2), Ki67, and cleaved caspase-3 (CC3), respectively, using immunocytochemistry as previously described ( de Lucia et al, 2020 ; Smeeth et al, in press ). For protocol details, antibodies used, and representative images see Supplementary Figure S1 .…”

Section: Methodsmentioning

confidence: 99%

“…Drosophila research shows that nutritional factors can influence the exit of neural hippocampal progenitors from quiescence (Chell and Brand, 2010;Spéder and Brand, 2014), and other nutritional-based changes to the hippocampal progenitor pool have been likewise demonstrated across other species (Spéder et al, 2011;Sakayori et al, 2013;Cavallucci et al, 2016). For instance, in humans, the nutrient-sensing pathways: the mammalian target of rapamycin (mTOR), sirtuin, and insulin-like growth factor 1, have all been associated with hippocampal progenitor maintenance (de Lucia et al, 2020). However, the influence of nutrition and meal content on the hippocampal progenitor pool occurs in a complex manner, with the nature of change dependent on the food groups consumed.…”

Section: Introductionmentioning

confidence: 99%

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Caffeine Compromises Proliferation of Human Hippocampal Progenitor Cells

Houghton

Preez

Lefèvre‐Arbogast

et al. 2020

Front. Cell Dev. Biol.

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The age-associated reduction in the proliferation of neural stem cells (NSCs) has been associated with cognitive decline. Numerous factors have been shown to modulate this process, including dietary components. Frequent consumption of caffeine has been correlated with an increased risk of cognitive decline, but further evidence of a negative effect on hippocampal progenitor proliferation is limited to animal models. Here, we used a human hippocampal progenitor cell line to investigate the effects of caffeine on hippocampal progenitor integrity and proliferation specifically. The effects of five caffeine concentrations (0 mM = control, 0.1 mM ∼ 150 mg, 0.25 mM ∼ 400 mg, 0.5 mM ∼ 750 mg, and 1.0 mM ∼ 1500 mg) were measured following acute (1 day) and repeated (3 days) exposure. Immunocytochemistry was used to quantify hippocampal progenitor integrity (i.e., SOX2-and Nestin-positive cells), proliferation (i.e., Ki67-positive cells), cell count (i.e., DAPI-positive cells), and apoptosis (i.e., CC3-positive cells). We found that progenitor integrity was significantly reduced in supraphysiological caffeine conditions (i.e., 1.0 mM ∼ 1500 mg), but relative to the lowest caffeine condition (i.e., 0.1 mM ∼ 150 mg) only. Moreover, repeated exposure to supraphysiological caffeine concentrations (i.e., 1.0 mM ∼ 1500 mg) was found to affect proliferation, significantly reducing % Ki67-positive cells relative to control and lower caffeine dose conditions (i.e., 0.1 mM ∼ 150 mg and 0.25 mM ∼ 400 mg). Caffeine treatment did not influence apoptosis and there were no significant differences in any measure between lower doses of caffeine (i.e., 0.1 mM, 0.25 mM, 0.5 mM)-representative of daily human caffeine intake-and control conditions. Our study demonstrates that dietary components such as caffeine can influence NSC integrity and proliferation and may be indicative of a mechanism by which diet affects cognitive outcomes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Caffeine Compromises Proliferation of Human Hippocampal Progenitor Cells

Houghton

Preez

Lefèvre‐Arbogast

et al. 2020

Front. Cell Dev. Biol.

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The serum metabolome mediates the concert of diet, exercise, and neurogenesis, determining the risk for cognitive decline and dementia

Preez

Lefèvre‐Arbogast

Houghton

et al. 2021

Alzheimer's & Dementia

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Introduction: Diet and exercise influence the risk of cognitive decline (CD) and dementia through the food metabolome and exercise-triggered endogenous factors, which use the blood as a vehicle to communicate with the brain. These factors might act in concert with hippocampal neurogenesis (HN) to shape CD and dementia.Methods: Using an in vitro neurogenesis assay, we examined the effects of serum samples from a longitudinal cohort (n = 418) on proxy HN readouts and their association with future CD and dementia across a 12-year period.Results: Altered apoptosis and reduced hippocampal progenitor cell integrity were associated with exercise and diet and predicted subsequent CD and dementia. The effects of exercise and diet on CD specifically were mediated by apoptosis.Discussion: Diet and exercise might influence neurogenesis long before the onset of CD and dementia. Alterations in HN could signify the start of the pathological process and potentially represent biomarkers for CD and dementia.

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Nurturing longevity through natural compounds: Where do we stand, and where do we go?

Todorova,

Savova,

Mihaylova

et al. 2024

Food Frontiers

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The revolution in aging research through the past decade has driven the progress in interventions that promote longevity. Dissection of the “old” hallmarks of aging has provided solid data for the definition of at least three “new” ones, opening avenues for the development of novel hallmark‐targeted pro‐longevity approaches. The quest for geroprotectors is of enormous interest with the ultimate goal of finding the alchemical stone that induces healthy aging and increases lifespan, pushing the limits of human longevity or even uncovering the absence of such limits. Several of the well‐appreciated geroprotectors that are recognized as longevity promoters are of natural origin such as metformin, resveratrol, aspirin, and spermidine. As the search for pharmacological modulators of healthspan and lifespan continues, numerous studies are focusing on the potential of plant secondary metabolites. The current review attempts to critically assess the available interventions and the breakthrough discoveries in the field of longevity research over the past decade. Correspondingly, novel approaches targeting the hallmarks of aging have been outlined, and the future goals in longevity research have been enlightened. Special emphasis has been placed on the potential of plant‐derived compounds as pro‐longevity agents.

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Lifestyle mediates the role of nutrient-sensing pathways in cognitive aging: cellular and epidemiological evidence

Cited by 31 publications

References 92 publications

Caffeine Compromises Proliferation of Human Hippocampal Progenitor Cells

Caffeine Compromises Proliferation of Human Hippocampal Progenitor Cells

The serum metabolome mediates the concert of diet, exercise, and neurogenesis, determining the risk for cognitive decline and dementia

Nurturing longevity through natural compounds: Where do we stand, and where do we go?

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