Lifelong exposure to caffeine increases anxiety-like behavior in adult mice exposed to tobacco smoke during adolescence

Gonçalves, Bruno da Silva Brandão; Leal-Rocha, Pedro Henrique; Manhães, Alex C.; Filgueiras, Cláudio C.; Abreu‐Villaça, Yael; Ribeiro‐Carvalho, Anderson

doi:10.1016/j.neulet.2018.12.026

Cited by 5 publications

(7 citation statements)

References 41 publications

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“…Neonatal CPF exposure also elicited significant alterations in the serotonergic system (Fig 4). At PN10, CPF exposure altered 5-HT 1a receptor (Treatment x Sex: F (1,19) (Fig 4C and 4D), in the brainstem, decreased values were identified in both sexes (Fig 4D, F (1,19) = 4.6, p = 0.045).…”

Section: Effects On the Serotonergic Systemmentioning

confidence: 91%

“…Anxiety-like behavior was initially investigated by using the EPM test. The test procedure is described in detail elsewhere [ 17 – 19 ]. The EPM is shaped like a plus sign and consists of two “open” (no walls, 5 × 28.5 cm) and two “closed” (5 × 28.5 × 14 cm) arms, arranged perpendicularly and elevated 50 cm above the floor.…”

Section: Methodsmentioning

confidence: 99%

“…Anxiety-like behavior was initially investigated by using the EPM test. The test procedure is described in detail elsewhere [17][18][19]. The EPM is shaped like a plus sign and consists of two…”

Section: Elevated Plus Maze (Epm)mentioning

confidence: 99%

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Mood-related behavioral and neurochemical alterations in mice exposed to low chlorpyrifos levels during the brain growth spurt

et al. 2020

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Organophosphates are among the most used pesticides. Particularly, chlorpyrifos (CPF) is responsible for a number of deleterious effects on brain development, which may program behavioral changes later in life. Here, we investigated whether a regimen of early low level CPF exposure that did not result in a significant inhibition of acetylcholinesterase (AChE) had deleterious effects on mood-related behaviors, as well as on cholinergic and serotonergic biomarkers in the mice brain. From the 3 rd to 9 th postnatal day (PN), male and female Swiss mice were subcutaneously injected with CPF. Mice were submitted to a battery of behavioral tests from PN60 to PN63: open field, elevated plus maze and forced swimming tests. The cholinergic and serotonergic biomarkers were assessed at PN10 and PN63. Our data indicated that early CPF exposure increased anxiety-like behavior in females and altered decision-making behavior in both sexes. Most biochemical alterations were sexdependent and restricted to females. At PN10, CPF female mice showed increased serotonin and choline transporter binding in cerebral cortex. Distinctively, in adult females, the effects indicated a hypoactive state: CPF exposure reduced 5-HT 1a receptor binding in cerebral cortex, as well as serotonin transporter binding and choline acetyltransferase activity in brainstem. Our results indicate that CPF exposure during the brain growth spurt deregulates serotonergic and cholinergic biomarkers. The effects are consistent with impaired synaptic function, may be related to long-term mood disorders and point out to higher female susceptibility.

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Section: Effects On the Serotonergic Systemmentioning

confidence: 91%

Section: Methodsmentioning

confidence: 99%

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Mood-related behavioral and neurochemical alterations in mice exposed to low chlorpyrifos levels during the brain growth spurt

et al. 2020

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“…The doses of caffeine used here, previously shown to antagonize adenosine receptors, 10,12,75–77 failed to affect food and fluid intake as well as body mass both in dams and offspring, which corroborates previous findings. 12,14 In contrast, the reduction in the time spent in the center of the OF indicates that lifetime exposure to the higher caffeine dose (0.3 g/L) increased anxiety-like behavior during adolescence. This result is in accordance with studies that evaluated the effects of caffeine on anxiety-like behaviors both during early development (prenatal/lactation) 9 and adolescence, 78,79 and with previous evidence that human caffeine consumption is linked to negative consequences on mood, mostly anxiety disorders.…”

Section: Discussionmentioning

confidence: 97%

“…In adolescent rodents, caffeine impairs decision-making and increases emotional reactivity and anxiety-like behavior. 11–14 In this sense, mood disorders and sleep disturbances have been associated with caffeine consumption by human teenagers. 15–17…”

Section: Introductionmentioning

confidence: 99%

Lifetime caffeine and adolescent nicotine exposure in mice: effects on anxiety-like behavior and reward

Dutra-Tavares

Ribeiro‐Carvalho

Nunes

et al. 2023

J Dev Orig Health Dis

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Caffeine consumption occurs throughout life, while nicotine use typically begins during adolescence, the period when caffeine-nicotine epidemiological association begins in earnest. Despite that, few studies in animal models parallel the pattern of coexposure that occurs in humans. Therefore, the neurobehavioral consequences of the association between these drugs remain unclear. Here, we exposed Swiss mice to lifetime caffeine. Caffeine solutions of 0.1 g/L (CAF0.1), 0.3 g/L (CAF0.3), or water (CTRL) were used as the sole liquid source, being offered to progenitors until weaning and, after that, directly to the offspring until the last day of adolescent behavioral evaluation. The open field test was used to evaluate acute effects of nicotine, of lifetime caffeine and of their interaction on locomotion and anxiety-like behavior, while the conditioned place preference test was used to assess the impact of caffeine on nicotine (0.5 mg/Kg, i.p.) reward. Frontal cerebral cortex dopamine content, dopamine turnover, and norepinephrine levels, as well as hippocampal serotonin 1A receptor expression were assessed. CAF0.3 mice exhibited an increase in anxiety-like behavior when compared to CAF0.1 and CTRL ones, but nicotine coexposure mitigated the anxiogenic-like caffeine-induced effect. Distinctively, caffeine had no effect on locomotion and failed to interfere with both nicotine-induced hyperactivity and place preference. There were no significant effects on dopaminergic and serotonergic markers. In conclusion, although caffeine did not affect nicotine reward, considering the strong association between anxiety disorders and tobacco consumption, caffeine-induced anxiety-like behavior advises limiting its consumption during development, including adolescence, as caffeine could be a risk factor to nicotine use.

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Caffeine and Anxiety-Like Behavior

Ribeiro‐Carvalho

Dutra-Tavares

Filgueiras

et al. 2022

Handbook of Substance Misuse and Addictions

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Lifelong exposure to caffeine increases anxiety-like behavior in adult mice exposed to tobacco smoke during adolescence

Cited by 5 publications

References 41 publications

Mood-related behavioral and neurochemical alterations in mice exposed to low chlorpyrifos levels during the brain growth spurt

Mood-related behavioral and neurochemical alterations in mice exposed to low chlorpyrifos levels during the brain growth spurt

Lifetime caffeine and adolescent nicotine exposure in mice: effects on anxiety-like behavior and reward

Caffeine and Anxiety-Like Behavior

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