Life in an unsuspected antibiotics world: River biofilms

Matviichuk, Olha; Mondamert, Leslie; Geffroy, Claude; Dagot, Christophe; Labanowski, Jérôme

doi:10.1016/j.watres.2023.119611

Cited by 9 publications

(4 citation statements)

References 70 publications

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“…Efflux pumps are found in almost every bacterial core genome and function in the secretion of substances from the cell, and not solely the removal of antibiotic compounds or other damaging cytoplasmic compounds (e.g., hydrogen ions) [ 76 ]. The increased abundance of efflux pump genes in antibiotic-treated samples and close association with integrons further suggests that an increased occurrence of HGT events (i.e., mutations, recombination) within riverine biofilm has been noted [ 77 , 78 ]. Given that ARGs and VFGs occurred even in biofilms grown without added antibiotics, its confirmed that genes encoding for resistance already existed or were previously acquired from natural riverine microbial communities.…”

Section: Resultsmentioning

confidence: 99%

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Environmentally Relevant Antibiotic Concentrations Exert Stronger Selection Pressure on River Biofilm Resistomes than AMR-Reservoir Effluents

Flores-Vargas,

Bergsveinson,

Korber

2024

Antibiotics

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Freshwater environments are primary receiving systems of wastewater and effluents, which carry low concentrations of antibiotics and antimicrobial-resistant (AMR) bacteria and genes. Aquatic microbial communities are thus exposed to environmentally relevant concentrations of antibiotics (ERCA) that presumably influence the acquisition and spread of environmental AMR. Here, we analyzed ERCA exposure with and without the additional presence of municipal wastewater treatment plant effluent (W) and swine manure run-off (M) on aquatic biofilm resistomes. Microscopic analyses revealed decreased taxonomic diversity and biofilm structural integrity, while metagenomic analysis revealed an increased abundance of resistance, virulence, and mobile element-related genes at the highest ERCA exposure levels, with less notable impacts observed when solely exposed to W or M effluents. Microbial function predictions indicated increased gene abundance associated with energy and cell membrane metabolism and heavy metal resistance under ERCA conditions. In silico predictions of increased resistance mechanisms did not correlate with observed phenotypic resistance patterns when whole communities were exposed to antimicrobial susceptibility testing. This reveals important insight into the complexity of whole-community coordination of physical and genetic responses to selective pressures. Lastly, the environmental AMR risk assessment of metagenomic data revealed a higher risk score for biofilms grown at sub-MIC antibiotic conditions.

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Section: Resultsmentioning

confidence: 99%

“…Pharmaceutical concentrations detected across river water source (RW) and AMR-reservoir inocula (M and W). References [ 5 , 7 , 10 , 13 , 34 , 66 , 77 , 82 , 83 , 84 , 85 , 86 , 87 , 88 ] are cited in the Supplementary Materials.…”

mentioning

confidence: 99%

Environmentally Relevant Antibiotic Concentrations Exert Stronger Selection Pressure on River Biofilm Resistomes than AMR-Reservoir Effluents

Flores-Vargas,

Bergsveinson,

Korber

2024

Antibiotics

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“…AR was detected in the river Tagus, the second biggest river in Spain, as well as the rivers Guadarrama, Jarama, Henares, and Manzanares (Blanco et al, 2023). In addition, rivers in Spain (Ebro), Italy (Arno), South Korea (Han), Taiwan (Sindian, Gaoping, Dahan, and Po), France (Seine), the United States (Ozark Stream), and Sweden (Hoje River) have also been the subject of research (Matviichuk et al, 2023; Singh et al, 2019). AR has also contaminated a number of urban aquifers in various regions of the world (Junaid et al, 2022).…”

Section: Fate Of Antibiotic Residues (Ar) In the Environmentmentioning

confidence: 99%

A comprehensive review on the fate and impact of antibiotic residues in the environment and public health: A special focus on the developing countries

Sikder,

Toha,

Anik

et al. 2024

Water Environment Research

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The widespread application of antibiotics in human and veterinary medicine has led to the pervasive presence of antibiotic residues in the environment, posing a potential hazard to public health. This comprehensive review aims to scrutinize the fate and impact of antibiotic residues, with a particular focus on the context of developing nations. The investigation delves into the diverse pathways facilitating the entry of antibiotics into the environment and meticulously examines their effects on human health. The review delineates the current state of antibiotic residues, evaluates their exposure in developing nations, and elucidates existing removal methodologies. Additionally, it probes into the factors contributing to the endurance and ecotoxicity of antibiotic residues, correlating these aspects with usage rates and associated mortalities in these nations. The study also investigates removal techniques for antibiotic residues, assessing their efficiency in environmental compartments. The concurrent emergence of antibiotic‐resistant bacteria, engendered by antibiotic residues, and their adverse ecological threats underscore the necessity for enhanced regulations, vigilant surveillance programs, and the adoption of sustainable alternatives. The review underlines the pivotal role of public education and awareness campaigns in promoting responsible antibiotic use. The synthesis concludes with strategic recommendations, strengthening the imperative for further research encompassing comprehensive monitoring, ecotoxicological effects, alternative strategies, socio‐economic considerations, and international collaborations, all aimed at mitigating the detrimental effects of antibiotic residues on human health and the environment.Practitioner Points Antibiotic residues are widely distributed in different environmental compartments. Developing countries use more antibiotics than developed countries. Human and veterinary wastes are one of the most responsible sources of antibiotic pollution. Antibiotics interact with biological systems and trigger pharmacological reactions at low doses. Antibiotics can be removed using modern biological, chemical, and physical–chemical techniques.

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“…In the environment, MPs cooccur with other contaminants of different classes and sources, such as pharmaceuticals and personal care products (PPCPs) associated with sewage discharges (Ziajahromi et al, 2016). Among PPCPs, antibiotic residues and antimicrobials are of particular concern to river biofilms due to their potential direct effects on bacterial community composition and/or their link with acquisition and spread of antimicrobial resistance (Balcázar et al, 2015;Drury et al, 2013a;Matviichuk et al, 2023;Rosi et al, 2018). It was reported that some of these contaminants can also be adsorbed on the surface of MPs, affecting their environmental fate and toxicity (Ziccardi et al, 2016;Santos et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan

Castaño-Ortiz,

Romero,

Cojoc

et al. 2024

Environmental Pollution

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Life in an unsuspected antibiotics world: River biofilms

Cited by 9 publications

References 70 publications

Environmentally Relevant Antibiotic Concentrations Exert Stronger Selection Pressure on River Biofilm Resistomes than AMR-Reservoir Effluents

Environmentally Relevant Antibiotic Concentrations Exert Stronger Selection Pressure on River Biofilm Resistomes than AMR-Reservoir Effluents

A comprehensive review on the fate and impact of antibiotic residues in the environment and public health: A special focus on the developing countries

Accumulation of polyethylene microplastics in river biofilms and effect on the uptake, biotransformation and toxicity of the antimicrobial triclosan

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