Life in acid: pH homeostasis in acidophiles

Baker‐Austin, Craig; Dopson, Mark

doi:10.1016/j.tim.2007.02.005

Cited by 505 publications

(390 citation statements)

References 55 publications

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“…Currently, various meticulous investigations on the molecular and physiological responses to acid shock are limited to cultivable oral bacteria [25], such as S. mutans , the gastric inhabitant E. coli [26] and Helicobacter pylori [27]. However, knowledge regarding the aciduric features of so far uncultivable microorganisms remains scarce.…”

Section: Discussionmentioning

confidence: 99%

Acid-resistant genes of oral plaque microbiome from the functional metagenomics

Zhang

Zheng

et al. 2018

Journal of Oral Microbiology

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Acid resistance is one of key properties assisting the survival of cariogenic bacteria in a dental caries environment, but only a few genes conferring acid resistance have been identified to data. Functional metagenomics provides a systematic method for investigating commensal DNA to identify genes that encode target functions. Here, the host strain Escherichia coli DH10B and a constructed bidirectional transcription vector pSKII+-lacZ contributed to the construction of a metagenomic library, and 46.6 Mb of metagenomic DNA was cloned from carious supragingival plaque of 8children along with screening for lethal functionality. The screen identified 2 positive clones that exhibited a similar aciduric phenotype to that of the positive controls. Bioinformatic analysis revealed that these two genes encoded an ATP/GTP-binding protein and a malate dehydrogenase. Moreover, we also performed functional screening of Streptococcus mutans, since it is one of the predominant cariogenic strains but was not identified in our initial screening. Five positive clones were retrieved. In conclusion, our improved functional metagenomics screening method helped in the identification of important acid resistance genes, thereby providing new insights into the mechanism underlying caries formation as well as in the prevention and treatment of early childhood caries (ECC).

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Section: Discussionmentioning

confidence: 99%

Acid-resistant genes of oral plaque microbiome from the functional metagenomics

Zhang

Zheng

et al. 2018

Journal of Oral Microbiology

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“…Firstly metal extraction from ores and this sustainable biotechnological process is becoming increasingly important because of its reduced and containable pollutant outputs. Acidophiles could also be a source of gene products; for example, acid-stable enzymes with applications as lubricants and catalysts (Baker-Austin and Dopson, 2007).…”

Section: Resultsmentioning

confidence: 99%

“…Both natural and man-made acidic environments on the Earth are commonly found in the sites like pyrite ores, solfatara fields and marine volcanic vents; the microorganisms that inhabited these areas are called acidophiles and have a pH optimum for growth pH <3 (Baker-Austin & Dopson, 2007). Acidophiles are most widely distributed in the bacterial and archaeal domains and contribute to numerous biogeochemical cycles including the iron and sulfur cycles.…”

Section: Acidophilic Archaeamentioning

confidence: 99%

“…Acidophiles optimally grow in low pH and metal-rich environments which might be quite resemble to volcanic aqueous conditions during Archaean and early Proterozoic periods. Therefore, it was suggested that acidophiles could represent primordial form of life from which more complex life have evolved (Baker-Austin & Dopson, 2007). Acidophiles are mostly found in isolated and inaccessible environments like geothermal vents.…”

Section: Acidophilic Archaeamentioning

confidence: 99%

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Archaeal Diversity and Their Biotechnological Potential

Özcan¹

2012

Genetic Diversity in Microorganisms

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“…For example, the lipid structure of the archaeal cell membrane is likely to be adapted for life under nutrient-poor conditions that are common within biologically hostile environments [23,24]. Modifications of the cytoplasmic membrane (along with other adaptations) are additionally known to help sustain pH homeostasis under extremes of pH [72,76]. While physiological and/or evolutionary factors are likely to have played a key role in determining the position of each catabolic group within the total parameter space for cell division (tables 2 and 3, figures 1-5), it is possible that some of the results reported in our study were influenced by purely physical or chemical processes.…”

Section: Discussionmentioning

confidence: 99%

Aerobically respiring prokaryotic strains exhibit a broader temperature–pH–salinity space for cell division than anaerobically respiring and fermentative strains

Harrison¹,

Dobinson²,

Freeman³

et al. 2015

J. R. Soc. Interface.

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Biological processes on the Earth operate within a parameter space that is constrained by physical and chemical extremes. Aerobic respiration can result in adenosine triphosphate yields up to over an order of magnitude higher than those attained anaerobically and, under certain conditions, may enable microbial multiplication over a broader range of extremes than other modes of catabolism. We employed growth data published for 241 prokaryotic strains to compare temperature, pH and salinity values for cell division between aerobically and anaerobically metabolizing taxa. Isolates employing oxygen as the terminal electron acceptor exhibited a considerably more extensive three-dimensional phase space for cell division (90% of the total volume) than taxa using other inorganic substrates or organic compounds as the electron acceptor (15% and 28% of the total volume, respectively), with all groups differing in their growth characteristics. Understanding the mechanistic basis of these differences will require integration of research into microbial ecology, physiology and energetics, with a focus on global-scale processes. Critical knowledge gaps include the combined impacts of diverse stress parameters on Gibbs energy yields and rates of microbial activity, interactions between cellular energetics and adaptations to extremes, and relating laboratory-based data to in situ limits for cell division.

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Life in acid: pH homeostasis in acidophiles

Cited by 505 publications

References 55 publications

Acid-resistant genes of oral plaque microbiome from the functional metagenomics

Acid-resistant genes of oral plaque microbiome from the functional metagenomics

Archaeal Diversity and Their Biotechnological Potential

Aerobically respiring prokaryotic strains exhibit a broader temperature–pH–salinity space for cell division than anaerobically respiring and fermentative strains

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