Life history, morphological variability and growth rates of the life phases of &lt;i&gt;Gracilaria tenuistipitata&lt;/i&gt; (Rhodophyta: Gracilariales) &lt;i&gt;in vitro&lt;/i&gt;

Barufi, José Bonomi; Oliveirã, Eurico C.; Plastino, Estela M.; Oliveira, Mariana Cabral de

doi:10.3989/scimar.2010.74n2297

Cited by 23 publications

(3 citation statements)

References 24 publications

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“…The life history of G. tenuistipitata var. liui was completed in vitro by Barufi et al [45], and the mitochondria genome was successfully sequenced in 2010 [46]. Recently, expressed sequence tags of G. tenuistipitata var.…”

Section: Resultsmentioning

confidence: 99%

Development of chloroplast simple sequence repeats (cpSSRs) for the intraspecific study of Gracilaria tenuistipitata (Gracilariales, Rhodophyta) from different populations

et al. 2014

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BackgroundGracilaria tenuistipitata is an agarophyte with substantial economic potential because of its high growth rate and tolerance to a wide range of environment factors. This red seaweed is intensively cultured in China for the production of agar and fodder for abalone. Microsatellite markers were developed from the chloroplast genome of G. tenuistipitata var. liui to differentiate G. tenuistipitata obtained from six different localities: four from Peninsular Malaysia, one from Thailand and one from Vietnam. Eighty G. tenuistipitata specimens were analyzed using eight simple sequence repeat (SSR) primer-pairs that we developed for polymerase chain reaction (PCR) amplification.FindingsFive mononucleotide primer-pairs and one trinucleotide primer-pair exhibited monomorphic alleles, whereas the other two primer-pairs separated the G. tenuistipitata specimens into two main clades. G. tenuistipitata from Thailand and Vietnam were grouped into one clade, and the populations from Batu Laut, Middle Banks and Kuah (Malaysia) were grouped into another clade. The combined dataset of these two primer-pairs separated G. tenuistipitata obtained from Kelantan, Malaysia from that obtained from other localities.ConclusionsBased on the variations in repeated nucleotides of microsatellite markers, our results suggested that the populations of G. tenuistipitata were distributed into two main geographical regions: (i) populations in the west coast of Peninsular Malaysia and (ii) populations facing the South China Sea. The correct identification of G. tenuistipitata strains with traits of high economic potential will be advantageous for the mass cultivation of seaweeds.

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Section: Resultsmentioning

confidence: 99%

Development of chloroplast simple sequence repeats (cpSSRs) for the intraspecific study of Gracilaria tenuistipitata (Gracilariales, Rhodophyta) from different populations

et al. 2014

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“…In general terms, the life cycle of Gracilaria cultures lasts 5-12 months [65]. Examples of this are the times reported for G. verrucosa (G. tenuistipitata) of 4.5 months [70] and five months for G. dominguensis [71].…”

Section: Growing From Spores Of Gracilaria Gracilismentioning

confidence: 96%

Culture of Gracilaria gracilis and Chondracanthus teedei from Vegetative Fragments in the Field and Carpospores in Laboratory

López-Campos

Pérez‐Lloréns

Barrena

et al. 2022

JMSE

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Gracilarioids and Gigartinales are of great economic importance due to the phycocolloids they contain in their cell wall and are used in different industries worldwide. Field and laboratory cultures of two species of red seaweeds (Gracilaria gracilis and Chondracanthus teedei), confirmed after DNA analysis, were carried out to foster the increasing use of this species in Spain as a food source. Vegetative cultures carried out in an open-lock gate within a traditional salina in the ay of Cadiz (Southern Spain) rendered maximum growth rates in April (3.64% day-1) for G. gracilis and in November (4.68% day−1) for C. teedei, the latter showing significant differences between the months of the year. For laboratory cultures, samples of the two species used for sporulation were obtained from tidal creeks in several nearby locations of the Bay. In order to grow fertile carposporophytes from spores, Provasoli enriched seawater medium (ES medium), Miquel A + B and f/2 were used as culture medium at a temperature of 18 °C and irradiance of 30 µmol m−2 s−1 in 12:12 h photoperiod. Both species developed a basal disc after 12–15 days in ES medium and Miquel A + B, and new microscopic seedlings were observed at 20–25 days in ES medium. With f/2 medium, no growth was observed after sporulation. The life cycle of G. gracilis was completed in ES medium over a period of 11 months with a mean growth rate of 3.28% day−1. The present study is an important step towards the development of seaweed cultivation in the Bay of Cadiz, especially in integrated multi-trophic cultivation in salinas as part of the more sustainable use of the marine resources in coastal communities.

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“…G. tenuistipitata has also been increasingly used as a model species in studies on red algal physiology (Carnicas et al 1999;Collén et al 2003;Falcão et al 2008Falcão et al , 2010García-Sánchez et al 1994;Pedersén 1992, 1993;Haglund et al 1996;Hu et al 1996;Lopes et al 1997Lopes et al , 2002Mercado et al 2001;Nyvall et al 1999;Nyvall Collén et al 2004;Skriptsova and Nabivailo 2009;Xu et al 2009;Yokoya et al 2004;Yu and Pedersén 1993). Another example of the growing interest in this alga is the sequencing of its chloroplast (Hagopian et al 2004) and mitochondrial (Takahashi 2010) genomes as well as completion of its life history in the laboratory (Barufi et al 2010).…”

Section: Introductionmentioning

confidence: 96%

Analysis of expressed sequence tags from the agarophyte Gracilaria tenuistipitata (Rhodophyta)

Collén¹,

Collén²,

Reis³

et al. 2011

J Appl Phycol

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A total of 3,631 expressed sequence tags (ESTs) were established from two size-selected cDNA libraries made from the tetrasporophytic phase of the agarophytic red alga Gracilaria tenuistipitata. The average sizes of the inserts in the two libraries were 1,600 bp and 600 bp, with an average length of the edited sequences of 850 bp. Clustering gave 2,387 assembled sequences with a redundancy of 53%. Of the ESTs, 65% had significant matches to sequences deposited in public databases, 11% to proteins without known function, and 35% were novel. The most represented ESTs were a Na/K-transporting ATPase, a hedgehog-like protein, a glycine dehydrogenase and an actin. Most of the identified genes were involved in primary metabolism and housekeeping. The largest functional group was thus genes involved in metabolism with 14% of the ESTs; other large functional categories included energy, transcription, and protein synthesis and destination. The codon usage was examined using a subset of the data, and the codon bias was found to be limited with all codon combinations used.

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Life history, morphological variability and growth rates of the life phases of <i>Gracilaria tenuistipitata</i> (Rhodophyta: Gracilariales) <i>in vitro</i>

Cited by 23 publications

References 24 publications

Development of chloroplast simple sequence repeats (cpSSRs) for the intraspecific study of Gracilaria tenuistipitata (Gracilariales, Rhodophyta) from different populations

Development of chloroplast simple sequence repeats (cpSSRs) for the intraspecific study of Gracilaria tenuistipitata (Gracilariales, Rhodophyta) from different populations

Culture of Gracilaria gracilis and Chondracanthus teedei from Vegetative Fragments in the Field and Carpospores in Laboratory

Analysis of expressed sequence tags from the agarophyte Gracilaria tenuistipitata (Rhodophyta)

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