Life cycle exposure to propiconazole reduces fecundity by disrupting the steroidogenic pathway and altering DNA methylation in zebrafish (Danio rerio)

Teng, Miaomiao; Chen, Xiangguang; Wang, Chen; Song, Min; Zhang, Jie; Bi, Sheng; Wang, Chengju

doi:10.1016/j.envint.2019.105384

Cited by 44 publications

(28 citation statements)

References 49 publications

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“…Changes in sex hormones and VTG also further indicated that the development of the gonads is inhibited by DCZ. Compared with previous studies(Li et al, 2019a;Teng et al, 2020), it is found that different triazole compounds (such as propiconazole and tebuconazole) have different effects on sex hormones in female/male zebra sh. In Teng et al's study, exposure to propiconazole (0.1, 5, and 250 µg/L) for 120 d signi cantly increased the content of E2 and decreased the content of T in both female and male sh(Teng et al, 2020).…”

contrasting

confidence: 76%

“…Compared with previous studies(Li et al, 2019a;Teng et al, 2020), it is found that different triazole compounds (such as propiconazole and tebuconazole) have different effects on sex hormones in female/male zebra sh. In Teng et al's study, exposure to propiconazole (0.1, 5, and 250 µg/L) for 120 d signi cantly increased the content of E2 and decreased the content of T in both female and male sh(Teng et al, 2020). On the contrary, in Li et al's study, exposure to tebuconazole (0.05, 0.20 and 0.50 mg/L) signi cantly decreased the content of E2 and increased the content of T in both female and male sh(Li et al, 2019a…”

contrasting

confidence: 76%

“…1, 1.0 or 10 µg/L DCZ signi cantly reduced CAN of eggs per female, TAN of spawning eggs, and fertilization rate. Similarly, decreases in the fecundity and fertilization capacity were also observed following treatment with other triazole fungicide, propiconazole (0.1, 5, and 250 µg/L), for 120 d (Teng et al, 2020). In vertebrates, the formation of gametes plays a very important role in fertility (Mitchell et al, 2020).…”

Section: Discussionmentioning

confidence: 84%

“…Similarly, the expression of other genes (gnrh, fshb, lhb, fshr, and lhr) in the HPGL axis also differs in gender. In Teng et al's study, the gender difference in the HPGL axis was also observed following treatment with DCZ for 21 d, and propiconazole for 120 d (Teng et al, 2020;Teng et al, 2018).…”

Section: Discussionmentioning

confidence: 90%

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WITHDRAWN: Exposure to Difenoconazole Induces Reproductive Toxicity of Zebrafish by Interfering Reproductive Behavior

Liu¹,

Zhou²,

Liu³

et al. 2022

Preprint

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Difenoconazole (DCZ) is a widely used triazole fungicide and negatively affects aquatic organisms and human health. However, data on the reproductive toxicity of DCZ are lacking. In this study, we used zebrafish (from 2–4 h postfertilization (hpf) to adulthood) as a model to evaluate whether DCZ at environmentally relevant concentrations (0.1, 1.0, and 10.0 µg/L) induce reproductive toxicity. Following exposure to DCZ, egg production and fertilization rate were reduced at 1.0 and 10.0 µg/L. A significant decrease in the proportion of mature gametes (late vitellogenic oocytes and spermatozoa) was also observed at 10.0 µg/L. The concentrations of 17β-Estradiol (E2), testosterone (T), and vitellogenin (VTG) were disrupted in females and males at 1.0 and 10.0 µg/L. Exposure to 10.0 µg/L DCZ significantly inhibited the contact time between female fish and male fish, and it was mainly achieved by affecting male fish. The transcription of genes involved in the hypothalamus-pituitary-gonad-liver (HPGL) axis was significantly changed after treatment with DCZ. Overall, these data showed that the endocrine disrupting effect of DCZ on the zebrafish HPGL axis inhibited the formation of gametes and disrupted reproductive behavior, thereby reducing fertility.

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contrasting

confidence: 76%

contrasting

confidence: 76%

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 90%

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WITHDRAWN: Exposure to Difenoconazole Induces Reproductive Toxicity of Zebrafish by Interfering Reproductive Behavior

Liu¹,

Zhou²,

Liu³

et al. 2022

Preprint

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“…However, the endocrine-disruptive effect of azole compounds on the reproductive function of most species is not limited to inhibition of aromatase production. Many studies have shown that triazoles, which include both cyproconazole and epoxiconazole, are also antagonists of estrogen and androgen receptors, in addition, they also exhibit weak agonistic affinity for the aryl-carbohydrate receptor [13,[21][22][23][24][25].…”

mentioning

confidence: 99%

Reproductive toxicity of triazole fungicides cyproconazole and epoxiconazole when exposed to male and female wistar rats during gametogenesis

Shepelskaya

Kolyanchuk

2021

OHNPU

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Aim. Studying the effect of generic pesticides cyproconazole (98 %) and two samples of epoxiconazole (epoxiconazole 1 — 95,75 % and epoxiconazole 2 — 98,7 %) on the reproductive system of male and female Wistar Han rats at the level of the organism when exposed during gametogenesis, identification and characterization of their hazard, as well as assessment of the risk of reproductive toxicity of these compounds. Materials and Methods. The test samples were administered daily (5 days a week) by oral gavage at doses of 0.2 and 2.0 mg/kg for cyproconazole and 0.5 and 2.0 mg/kg for epoxiconazoles during 11 weeks for males, and 10 weeks for females. Also, there were kept intact males and females, intended for crossover mating with experimental animals. After the end of the exposure, functional indicators of the state of the gonads and the ability of animals to reproduce offspring were studied. The duration and the frequency of each stage of the estrous cycle in female rats and the number of motile sperm, the total amount of sperm and the number of abnormal forms of germ cells of the male rats were studied. The reproductive function state in females was evaluated on day 20th of pregnancy. Thereby the number of corpora lutea in the ovaries, number of alive, dead and resorbed foetuses and embryos, the foetus weight, total weight of litters were registered. The studies were carried out in accordance with the recommendations of the Bioethics Commission and the Centre’s standard operating procedures, developed in accordance with the recommendations and requirements of Good Laboratory Practice (GLP). Conclusions. Test substances at a maximum dose of 2.0 mg/kg of body weight have reproductive toxicity and endocrine-disruptive effect, exerting a significant antiandrogenic effect on males and antiestrogenic effect on female rats. No-observed-adverse-effect-level (NOАEL) for gonadal and reproductive toxicity for male and female Wistar Han rats were established. They are 0.2 mg/kg body weight for cyproconazole and 0.5 mg/kg body weight for epoxiconazole. Key Words: azole fungicides, cyproconazole, epoxiconazole, reproductive toxicity, antiandrogenic and antiestrogenic effects, Wistar Han rats.

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Estrogenic regulation of claudin 5 and tight junction protein 1 gene expression in zebrafish: A role on blood–brain barrier?

Pellegrini,

Fernezelian,

Malleret

et al. 2023

J of Comparative Neurology

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The blood–brain barrier (BBB) is a physical interface between the blood and the brain parenchyma, playing key roles in brain homeostasis. In mammals, the BBB is established thanks to tight junctions between cerebral endothelial cells, involving claudin, occludin, and zonula occludens proteins. Estrogens have been documented to modulate BBB permeability. Interestingly, in the brain of zebrafish, the estrogen‐synthesizing activity is strong due to the high expression of Aromatase B protein, encoded by the cyp19a1b gene, in radial glial cells (neural stem cells). Given the roles of estrogens in BBB function, we investigated their impact on the expression of genes involved in BBB tight junctions. We treated zebrafish embryos and adult males with 17β‐estradiol and observed an increased cerebral expression of tight junction and claudin 5 genes in adult males only. In females, treatment with the nuclear estrogen receptor antagonist (ICI182,780) had no impact. Interestingly, telencephalic injuries performed in males decreased tight junction gene expression that was partially reversed with 17β‐estradiol. This was further confirmed by extravasation experiments of Evans blue showing that estrogenic treatment limits BBB leakage. We also highlighted the intimate links between endothelial cells and neural stem cells, suggesting that cholesterol and peripheral steroids could be taken up by endothelial cells and used as precursors for estrogen synthesis by neural stem cells. Together, our results show that zebrafish provides an alternative model to further investigate the role of steroids on the expression of genes involved in BBB integrity, both in constitutive and regenerative physiological conditions. The link we described between capillaries endothelial cells and steroidogenic neural cells encourages the use of this model in understanding the mechanisms by which peripheral steroids get into neural tissue and modulate neurogenic activity.

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Life cycle exposure to propiconazole reduces fecundity by disrupting the steroidogenic pathway and altering DNA methylation in zebrafish (Danio rerio)

Cited by 44 publications

References 49 publications

WITHDRAWN: Exposure to Difenoconazole Induces Reproductive Toxicity of Zebrafish by Interfering Reproductive Behavior

WITHDRAWN: Exposure to Difenoconazole Induces Reproductive Toxicity of Zebrafish by Interfering Reproductive Behavior

Reproductive toxicity of triazole fungicides cyproconazole and epoxiconazole when exposed to male and female wistar rats during gametogenesis

Estrogenic regulation of claudin 5 and tight junction protein 1 gene expression in zebrafish: A role on blood–brain barrier?

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