Life cycle and population growth rate of Caenorhabditis elegans studied by a new method

Muschiol, Daniel; Schroeder, Fabian; Traunspurger, Walter

doi:10.1186/1472-6785-9-14

Cited by 85 publications

(67 citation statements)

References 54 publications

(57 reference statements)

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“…2008; Muschiol et al. 2009; Lancaster et al. 2012; Darby and Herman 2014), but the resulting estimates of population growth rates have never been tested experimentally.…”

Section: Discussionmentioning

confidence: 99%

Association with pathogenic bacteria affects life‐history traits and population growth inCaenorhabditis elegans

Díaz

Mooring

Rens

et al. 2015

Ecology and Evolution

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Determining the relationship between individual life-history traits and population dynamics is an essential step to understand and predict natural selection. Model organisms that can be conveniently studied experimentally at both levels are invaluable to test the rich body of theoretical literature in this area. The nematode Caenorhabditis elegans, despite being a well-established workhorse in genetics, has only recently received attention from ecologists and evolutionary biologists, especially with respect to its association with pathogenic bacteria. In order to start filling the gap between the two areas, we conducted a series of experiments aiming at measuring life-history traits as well as population growth of C. elegans in response to three different bacterial strains: Escherichia coli OP50, Salmonella enterica Typhimurium, and Pseudomonas aeruginosa PAO1. Whereas previous studies had established that the latter two reduced the survival of nematodes feeding on them compared to E. coli OP50, we report for the first time an enhancement in reproductive success and population growth for worms feeding on S. enterica Typhimurium. Furthermore, we used an age-specific population dynamic model, parameterized using individual life-history assays, to successfully predict the growth of populations over three generations. This study paves the way for more detailed and quantitative experimental investigation of the ecology and evolution of C. elegans and the bacteria it interacts with, which could improve our understanding of the fate of opportunistic pathogens in the environment.

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“…2008; Muschiol et al. 2009; Lancaster et al. 2012; Darby and Herman 2014), but the resulting estimates of population growth rates have never been tested experimentally.…”

Section: Discussionmentioning

confidence: 99%

Association with pathogenic bacteria affects life‐history traits and population growth inCaenorhabditis elegans

Díaz

Mooring

Rens

et al. 2015

Ecology and Evolution

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“…39 In C. elegans , germ cell apoptosis commences in early adulthood and increases over time. 24 To exclude the changes of background value, we investigated the developmental effects by DPE plus UVA at different stages.…”

Section: Resultsmentioning

confidence: 99%

Synergistic Effects Induced by a Low Dose of Diesel Particulate Extract and Ultraviolet-A in Caenorhabditis elegans: DNA Damage-Triggered Germ Cell Apoptosis

Guo

Bian

Liang

et al. 2014

Chem. Res. Toxicol.

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Diesel exhaust has been classified as a potential carcinogen and is associated with various health effects. A previous study showed that the doses for manifesting the mutagenetic effects of diesel exhaust could be reduced when coexposed with ultraviolet-A (UVA) in a cellular system. However, the mechanisms underlying synergistic effects remain to be clarified, especially in an in vivo system. In the present study, using Caenorhabditis elegans (C. elegans) as an in vivo system we studied the synergistic effects of diesel particulate extract (DPE) plus UVA, and the underlying mechanisms were dissected genetically using related mutants. Our results demonstrated that though coexposure of wild type worms at young adult stage to low doses of DPE (20 μg/mL) plus UVA (0.2, 0.5, and 1.0 J/cm2) did not affect worm development (mitotic germ cells and brood size), it resulted in a significant induction of germ cell death. Using the strain of hus-1::gfp, distinct foci of HUS-1::GFP was observed in proliferating germ cells, indicating the DNA damage after worms were treated with DPE plus UVA. Moreover, the induction of germ cell death by DPE plus UVA was alleviated in single-gene loss-of-function mutations of core apoptotic, checkpoint HUS-1, CEP-1/p53, and MAPK dependent signaling pathways. Using a reactive oxygen species (ROS) probe, it was found that the production of ROS in worms coexposed to DPE plus UVA increased in a time-dependent manner. In addition, employing a singlet oxygen (1O2) trapping probe, 2,2,6,6-tetramethyl-4-piperidone, coupled with electron spin resonance analysis, we demonstrated the increased 1O2 production in worms coexposed to DPE plus UVA. These results indicated that UVA could enhance the apoptotic induction of DPE at low doses through a DNA damage-triggered pathway and that the production of ROS, especially 1O2, played a pivotal role in initiating the synergistic process.

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“…Strain N2, which was isolated from mushroom compost near Bristol (UK) by L.N. Staniland, is the canonical 'wild-type' C. elegans strain and is used in laboratories throughout the world (Muschiol et al 2009). Nematodes were maintained on 9-mm nematode growth medium (NGM) agar plates containing (per litre of pure water): NaCl (3.0 g), agar (17 g), peptone (2.5 g), CaCl 2 (111 mg), cholesterol (5 mg in 1 ml ethanol), MgSO 4 (247 mg), and K 3 PO 4 (3.35 g).…”

Section: Fungus and Nematodementioning

confidence: 99%

Effects of abscisic acid and nitric oxide on trap formation and trapping of nematodes by the fungus Drechslerella stenobrocha AS6.1

Lai

Wang

et al. 2011

Fungal Biology

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Life cycle and population growth rate of Caenorhabditis elegans studied by a new method

Cited by 85 publications

References 54 publications

Association with pathogenic bacteria affects life‐history traits and population growth inCaenorhabditis elegans

Association with pathogenic bacteria affects life‐history traits and population growth inCaenorhabditis elegans

Synergistic Effects Induced by a Low Dose of Diesel Particulate Extract and Ultraviolet-A in Caenorhabditis elegans: DNA Damage-Triggered Germ Cell Apoptosis

Effects of abscisic acid and nitric oxide on trap formation and trapping of nematodes by the fungus Drechslerella stenobrocha AS6.1

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