Lidocaine Inhibits Myoblast Cell Migration and Myogenic Differentiation Through Activation of the Notch Pathway

Ling, Xiangtian; Ma, Xinqi; Kuang, Xiaying; Zou, Yuxiu; Zhang, Han; Tang, Hongfeng; Du, Han; Zhu, Binbin; Huang, Hao; Xia, Qing; Chen, Minghao; Mao, Danyi; Chen, Dongli; Shen, Huangxuan; Yan, Jianhua

doi:10.2147/dddt.s290002

Cited by 3 publications

(3 citation statements)

References 32 publications

(36 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Local anesthetics may induce myotoxicity; the higher the concentrations and the longer the duration of exposure to local anesthetics, the greater the damage to the muscles. [2][3][4] Clinically relevant local anesthetics concerning myotoxicity included lidocaine, ropivacaine, and bupivacaine, in increasing order of toxicity. The addition of epinephrine to local anesthetics may also increase myotoxicity.…”

Section: Myotoxicitymentioning

confidence: 99%

Cytotoxicity of Local Anesthetics on Bone, Joint, and Muscle Tissues: A Narrative Review of the Current Literature

Zhang¹,

Li²,

Yao³

et al. 2023

JPR

View full text Add to dashboard Cite

Background:Local anesthetics are commonly used in surgical procedures to control pain in patients. Whilst the cardiotoxicity and neurotoxicity of local anesthetics have received much attention, the cytotoxicity they exert against bone, joint, and muscle tissues has yet to be well recognized. Objective: This review aimed to raise awareness regarding how local anesthetics may cause tissue damage and provide a deeper understanding of the mechanisms of local anesthetic-induced cytotoxicity. We summarized the latest progress on the cytotoxicity of local anesthetics and the underlying mechanisms and discussed potential strategies to reduce it. Findings: We found that the toxic effects of local anesthetics on bone, joint, and muscle tissues were time-and concentrationdependent in vitro. Local anesthetics induced apoptosis, necrosis, and autophagy through specific cellular pathways. Altogether, this review indicates that toxicity of local anesthetics may be avoided by rationally selecting the appropriate anesthetic, limiting the total amount, and determining the lowest effective concentration and duration.

show abstract

Section: Myotoxicitymentioning

confidence: 99%

Cytotoxicity of Local Anesthetics on Bone, Joint, and Muscle Tissues: A Narrative Review of the Current Literature

Zhang¹,

Li²,

Yao³

et al. 2023

JPR

View full text Add to dashboard Cite

show abstract

“…A study indicated that corticosteroids prevent migration of tenocytes and are associated with decreased α‐SM expression 15 . Lidocaine has been reported to significantly mitigate platelet‐rich plasma's beneficial effects on tenocytes and inhibit the migration of myoblasts 16,17 …”

Section: Introductionmentioning

confidence: 99%

“…15 Lidocaine has been reported to significantly mitigate platelet-rich plasma's beneficial effects on tenocytes and inhibit the migration of myoblasts. 16,17 Tendon injury healing occurs in three stages: the inflammation stage, the regeneration stage, and the remodeling and maturation stage. 2 In the first of these stages, tenocytes infiltrate the repair site, actively and rapidly reproduce, and deposit extracellular matrix (ECM) components within affected tissue.…”

mentioning

confidence: 99%

Lidocaine inhibits migration of tenocytes by downregulating focal adhesion kinase and paxillin phosphorylation

Chang,

Chen,

et al. 2023

Journal Orthopaedic Research

View full text Add to dashboard Cite

Lidocaine is the most frequently applied local infiltration anesthetic agent for treating tendinopathies. However, studies have discovered lidocaine to negatively affect tendon healing. In the current study, the molecular mechanisms and effects of lidocaine on tenocyte migration were evaluated. We treated tenocytes intrinsic to the Achilles tendons of Sprague–Dawley rats with lidocaine. The migration ability of cells was analyzed using electric cell‐substrate impedance sensing (ECIS) and scratch wound assay. We then used a microscope to evaluate the cell spread. We assessed filamentous actin (F‐actin) cytoskeleton formation through immunofluorescence staining. In addition, we used Western blot analysis to analyze the expression of phospho‐focal adhesion kinase (FAK), FAK, phospho‐paxillin, paxillin, and F‐actin. We discovered that lidocaine had an inhibitory effect on the migration of tenocytes in the scratch wound assay and on the ECIS chip. Lidocaine treatment suppressed cell spreading and changed the cell morphology and F‐actin distribution. Lidocaine reduced F‐actin formation in the tenocyte during cell spreading; furthermore, it inhibited phospho‐FAK, F‐actin, and phospho‐paxillin expression in the tenocytes. Our study revealed that lidocaine inhibits the spread and migration of tenocytes. The molecular mechanism potentially underlying this effect is downregulation of F‐actin, phospho‐FAK, and phospho‐paxillin expression when cells are treated with lidocaine.

show abstract

Orthobiologic Interventions for Muscle Injuries

Stephens

Nussbaum²,

Onishi³

2023

Physical Medicine and Rehabilitation Clinics of North America

View full text Add to dashboard Cite

Lidocaine Inhibits Myoblast Cell Migration and Myogenic Differentiation Through Activation of the Notch Pathway

Cited by 3 publications

References 32 publications

Cytotoxicity of Local Anesthetics on Bone, Joint, and Muscle Tissues: A Narrative Review of the Current Literature

Cytotoxicity of Local Anesthetics on Bone, Joint, and Muscle Tissues: A Narrative Review of the Current Literature

Lidocaine inhibits migration of tenocytes by downregulating focal adhesion kinase and paxillin phosphorylation

Orthobiologic Interventions for Muscle Injuries

Contact Info

Product

Resources

About