Lichtmikroskopische und elektronenoptische Untersuchungen morphologischer Veränderungen von Muskelfasern bei freier autologer Muskeltransplantation

Abstract

The extensor digitorum longus muscle of the rat was denervated and transplanted onto the vastus lateralis muscle of the same limb 1 or 2 weeks after denervation. The morphological changes appearing after transplantation were examined by light and electron microscopy. Only three to four superficial layers of muscle fibres remained unchanged after transplantation. The other parts of the transplanted muscle degenerated nearly entirely during the first 10 days. The myofibrils were removed by macrophages, thus within the tubes of basal lamina no contractile material could be observed. In a second period, regeneration of myofilaments took place. 6 weeks after transplantation, newly formed muscle could be observed, capable of normal function. The diameter of the fibres, however, was smaller and the connective tissue between the fibres markedly increased as compared to the normal muscle of the other side. The importance of the findings for muscle transplantation, especially the relationship between satellite cells and myoblasts, is discussed.

Branched myofibers in long‐term whole muscle transplants: A quantitative study

“…Using this system, described by Studitsky and Bosova (1960) and Bosova (1962) and modified by Carlson and Gutmann (1974), entire muscles, cut free of their tendons, nerves, and vascular supply, are implanted into a suitably prepared muscle bed. Subsequent to transplantation the myofibers of the entire graft, except for a thin rim of surviving peripheral fibers, undergo total fiber necrosis (Carlson and Gutmann, 1975;Lischka et al, 1977). The peripheral fibers can be induced to undergo necrosis by soaking the graft in Marcaine (Breon Laboratories), a known myotoxic agent (Benoit and Belt, 1970;Libelius et al, 1970).…”

Secondary myogenesis of normal muscle produces abnormal myotubes

“…No microsurgical procedures are performed to anastomose the blood vessels or nerves of the grafted muscle to the surrounding vasculature or nerves. With the exception of a few fibers at the periphery of the implant, all of the myofibers become ischemic and undergo total fiber necrosis (Carlson and Gutmann, 1975;Lischka et al, 1977). Macrophages remove the necrotic fibers (Snow, 1977a1, leaving only their relatively intact basal lamina (Carlson et al, 1979;Hansen-Smith and Carlson, 1979).…”

Time Course of Alterations in Muscle Transfers with Microneurovascular Anastomoses

“…Power and excursion are the main interests, especially in the extremities. 3 ' 5910 In the muscle graft without microsurgical repair of the vessels, a totally different process takes place, as previously described extensively by Carlson, 24 " 26 Lischka, et al, 27 and Cedars, et al, 28 among others. The missing blood supply in the immediate postoperative period causes an extensive field of degeneration and necrosis in a large muscle transfer like the rectus femoris muscle (Fig.…”