Lichen secondary metabolite evernic acid as potential quorum sensing inhibitor against Pseudomonas aeruginosa

Gökalsın, Barış; Sesal, Nüzhet Cenk

doi:10.1007/s11274-016-2105-5

Cited by 29 publications

(17 citation statements)

References 38 publications

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“…E. prunastri and evernic acid have already been studied for their antiproliferative and antimicrobial activities [ 31 , 32 ]. In a recent publication, the effect of evernic acid on bacterial biofilm has been evaluated and this depside has demonstrated a capacity to inhibit Pseudomonas aeruginosa quorum sensing systems [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Antifungal and Anti-Biofilm Activities of Acetone Lichen Extracts against Candida albicans

et al. 2017

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Candida albicans is a commensal coloniser of the human gastrointestinal tract and an opportunistic pathogen, especially thanks to its capacity to form biofilms. This lifestyle is frequently involved in infections and increases the yeast resistance to antimicrobials and immune defenses. In this context, 38 lichen acetone extracts have been prepared and evaluated for their activity against C. albicans planktonic and sessile cells. Minimum inhibitory concentrations of extracts (MICs) were determined using the broth microdilution method. Anti-biofilm activity was evaluated using tetrazolium salt (XTT) assay as the ability to inhibit the maturation phase (anti-maturation) or to eradicate a preformed 24 h old biofilm (anti-biofilm). While none of the extracts were active against planktonic cells, biofilm maturation was limited by 11 of the tested extracts. Seven extracts displayed both anti-maturation and anti-biofilm activities (half maximal inhibitory concentrations IC50_mat and IC50_biof ≤ 100 µg/mL); Evernia prunastri and Ramalina fastigiata were the most promising lichens (IC50_mat < 4 µg/mL and IC50_biof < 10 µg/mL). Chemical profiles of the active extracts performed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) have been analyzed. Depsides, which were present in large amounts in the most active extracts, could be involved in anti-biofilm activities. This work confirmed that lichens represent a reservoir of compounds with anti-biofilm potential.

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Section: Resultsmentioning

confidence: 99%

Antifungal and Anti-Biofilm Activities of Acetone Lichen Extracts against Candida albicans

et al. 2017

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“…gordonii and P. gingivalis are oral bacteria initiating the formation of dental plaques contributing in turn to the onset of dental caries and periodontal diseases as well as their progression [10]. We have previously demonstrated that these bacteria are sensitive to new drugs which are of natural origin as they are derived from lichen secondary metabolites [7,25]. However, its antibacterial mechanisms are still unknown.…”

Section: Resultsmentioning

confidence: 99%

Lichen butyrolactone derivatives disrupt oral bacterial membrane

Sweidan

Smida²,

Chollet-Krugler³

et al. 2019

Fitoterapia

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We have previously demonstrated that out of the butyrolactones series synthesized based on the natural lichen metabolite lichesterinic acid, compound (B-13) was the most effective against oral bacteria. However, its antibacterial mechanism is still unknown. In this study, we have investigated its bacterial localization by synthesizing a fluorescently labeled B-13 with NBD while maintaining its antibacterial activity. We showed that this compound binds to Streptococcus gordonii cell surface, as demonstrated by HPLC analysis. By adhering to cell surface, B-13 induced cell wall disruption leading to the release of bacterial constituents and consequently, the death of S. gordonii, a Gram-positive bacterium. A Gram-negative counterpart, Porphyromanas gingivalis, showed also cracked and ruptured cells in the presence of B-13. Besides, we also demonstrated that the analog of B-13, B-12, has also induced disruption of P. gingivalis and S. gordonii. This study revealed that butyrolactones can be considered as potent antibacterial compounds against oral pathogens causing medical complications.

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“…Significance was accepted when the P value was < 0.05. There was no difference between the bactericidal activities on these two bacteria [18] 16sRNA CGGTCCAGACTCCTACGGGAGGCAGC A GCGTGGACTACCAGGGTATCTAATCC [19] mechanism of the bactericidal capabilities of AMPs mainly includes the destructive effects on bacterial cell walls and membranes. Besides their direct antimicrobial function on susceptible bacteria, AMPs have multiple roles as mediators in the inflammations and other diseases [21].…”

Section: Discussionmentioning

confidence: 99%

Effects of human β-defensin 3 fused with carbohydrate-binding domain on the function of type III secretion system in Pseudomonas aeruginosa PA14

Liu

Dong

et al. 2020

Braz J Microbiol

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Antimicrobial peptides are considered to be one of the candidate antimicrobial agents for antibiotic-resistant bacterial infection in the future. The effects of antimicrobial peptide hBD3-CBD on Pseudomonas aeruginosa PA14 and PA14 ΔexsA were analyzed by the bactericidal effects, hemolysis assays, pyocyanin pigment productions, and virulence factor expressions (exoU, exoS, hcnA, and lasB). Pyocyanin production and virulence factor expressions are important features of the type III secretion system in Pseudomonas aeruginosa. HBD3-CBD killed PA14 and PA14 ΔexsA with similar efficiency; it lowered the hemolysis levels of PA14 and PA14 ΔexsA and reduced the pyocyanin production, biofilm formation, and exoU, exoS, and lasB expressions in PA14. Compared with PA14, PA14 ΔexsA showed a lower hemolysis effect, pyocyanin production, exoU, and lasB expressions. The effects of hBD3-CBD on the PA14 toxin secretion were similar to the changes in the type III secretion system mutant isolate PA14 ΔexsA. Our results demonstrated that the type III secretion system was involved in the biological functions on PA 14 from hBD3-CBD. Keywords Antimicrobial peptide. hBD3. Pseudomonas aeruginosa. T3SS Antimicrobial peptides (AMPs) are polypeptides with broadspectrum antimicrobial activity against bacteria, fungi, and other microorganisms [1]. Their potent activity at low concentrations against many pathogenic organisms, including multiresistant strains, makes AMPs attractive candidates as antimicrobial agents to many inflammatory and infectious diseases [2]. Human beta-defensin-3 (hBD3) is a multifunctional peptide and widely expressed in oral and other tissues. The antiinfective effects of this polypeptide have been confirmed by several studies [3-5].

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Lichen secondary metabolite evernic acid as potential quorum sensing inhibitor against Pseudomonas aeruginosa

Cited by 29 publications

References 38 publications

Antifungal and Anti-Biofilm Activities of Acetone Lichen Extracts against Candida albicans

Antifungal and Anti-Biofilm Activities of Acetone Lichen Extracts against Candida albicans

Lichen butyrolactone derivatives disrupt oral bacterial membrane

Effects of human β-defensin 3 fused with carbohydrate-binding domain on the function of type III secretion system in Pseudomonas aeruginosa PA14

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