Libyan <i>Thymus capitatus</i>
 essential oil: antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties

Džamić, Ana M.; Nikolić, Biljana; Giweli, Abdulhmid; Mitić-Ćulafić, Dragana; Sokóvić, Marina; Ristić, Mihailo; Knežević-Vukčević, Jelena; Marin, Petar D.

doi:10.1111/jam.12864

Cited by 45 publications

(24 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is another important finding since L. monocytogenes and many other food-borne pathogens need to cross the epithelial barrier of the intestine to cause a systemic disease. The first step in this process is attachment to host intestinal cells, thus the search for natural products with anti-adhesive properties is encouraged [23,40]. The ability of J. communis products to inhibit adhesion of Campylobacter jejuni to polystyrene has been recently reported [41].…”

Section: Discussionmentioning

confidence: 99%

“…The microdilution assay, performed as previously described [23], was used to evaluate the antifungal potential. Briefly, the fungal spore suspensions (1.0 × 10 5 CFU mL -1 ) were added to each well containing graded concentrations of test substances.…”

Section: Microdilution Assaymentioning

confidence: 99%

“…Cytotoxicity was determined by the MTT reduction assay, performed as previously described [23]. Briefly, the assay was performed on HT-29 and HCT116 cells inoculated in 96-well plates at a density of 5x10 4 cells/ well and incubated until they formed a monolayer; EO and PDW were serially two-fold diluted in tested concentration ranges (0.016-0.500 and 0.313-20 mg mL -1 , respectively).…”

Section: Cytotoxicity Assaymentioning

confidence: 99%

“…Both HT-29 and HCT116 cells were used as a model of colon epithelium. The adhesion-inhibition assay was performed using a slightly modified protocol previously described [23]. Colon cells were inoculated into 12-well plates at a density 8x10 4 cells/well and incubated at 37°C in 5% CO 2 to form a monolayer.…”

Section: In Vitro Adhesion-inhibition Assaymentioning

confidence: 99%

See 3 more Smart Citations

Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants

Nikolić

Vasilijević

Ćirić

et al. 2019

ARCH BIOL SCI BELGRA

View full text Add to dashboard Cite

Previously chemically characterized Juniperus communis essential oil (EO) and post-distillation waste (PDW) were tested for cytotoxicity and antimicrobial activity against food contaminants. Microdilution assay showed that PDW induced moderate antifungal (minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values, ranging between 0.118-0.900 mg mL-1), and an antibacterial effect against Listeria monocytogenes (MIC and minimum bactericidal concentration (MBC) were 0.39 and 0.74 mg mL-1 , respectively). Combinations of EO/PDW with selected antibiotics induced synergistic antilisterial activity in the checkerboard assay. The MTT assay determined that cytotoxicity against colon cancer cells was high for the EO but negligible for PDW (IC 50 values were 0.087-0.106 and 1.450-6.840 mg mL-1 , respectively). The selectivity indices indicated high selectivity of PDW against tested fungi and L. monocytogenes. In the adhesion-inhibition assay, PDW reduced in vitro adhesion of L. monocytogenes to colon cells (29-62% of inhibition). In conclusion, PDW exhibited an antimicrobial effect against important food spoilage and poisoning fungi and L. monocytogenes, and also reduced in vitro adhesion of L. monocytogenes to colon cells. The results indicate that J. communis PDW could be considered as natural preservative against food spoilage and poisonous fungi, and as an adjuvant to conventional therapy of listeriosis.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Microdilution Assaymentioning

confidence: 99%

Section: Cytotoxicity Assaymentioning

confidence: 99%

Section: In Vitro Adhesion-inhibition Assaymentioning

confidence: 99%

See 2 more Smart Citations

Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants

Nikolić

Vasilijević

Ćirić

et al. 2019

ARCH BIOL SCI BELGRA

View full text Add to dashboard Cite

show abstract

“…and their phenolic components (thymol and p-cymene) against the species of yeasts and filamentous fungi isolates. 14,15,30 A similar study reported that both thymol and the essential oil of T. vulgaris, whose main components are p-cymene (36.5%) and thymol (33.0%), showed strong fungicidal and/or fungistatic activities against Aspergillus spp., Penicillium spp., Cladosporium spp., Trichoderma spp., Mucor spp. and Rhizopus spp.…”

Section: Candida Kefyr Fusarium Sp Aspergillus Flavus and Aspergillmentioning

confidence: 97%

Evaluation of antifungal activity and cytotoxicity of Thymus vulgaris essential oil

Al-Shahrani¹,

Mahfoud²,

Riyasdeen³

et al. 2017

View full text Add to dashboard Cite

Objective: To evaluate the antimicrobial properties of Thymus vulgaris against seven pathogenic fungi strains and its cytotoxic effect on a human breast cancer cell line (MDA-MB-231) Methods: The susceptibility test was carried out in terms of by determining minimal inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) using micro-dilution methods and additional end point by using MTS chromogenic assay. Cytotoxicity activity was assessed using MTT assay, the viability and the IC50 were used to evaluate this test. Results: All tested fungal strains were sensitive to the (Thymus vulgaris essential oil) TEO and this inhibitory effect is dose-dependent. A remarkable (p<0.001) inhibitory effect at concentrations 2.5-10mg/ml for all tested organisms comparable with control was found. The MIC 50 values ranged between 0.5 to 5mg/ml. The MFC values varied from 2.5-10 mg/mL and were equal to MIC values for majority of the tested organisms. As far as the antifungal activity is concern, out of the seven strains tested, Candida kefyr exhibited the highest sensitivity with minimal inhibition growth 2.5mg/ml whereas, Aspergillus niger exhibited the lowest inhibition ≥10mg/ml. Thymus vulgaris oil inhibited proliferation of MDA-MD-231 cancer cells in a time-and dose dependent manner. IC 50 value of the complex was found to be time dependent. At 24h treatment, the IC 50 values ranging between 108-115μg/ml, while at 48h treatment the values were between 71-78μg/ml against cancer cell. Conclusion: Thymus vulgaris essential oil exhibited significant antifungal and cytotoxic effects on living cells. Therefore, it can be used as alternative antifungal natural substances and also play a significant role in discovery of the new drugs.

show abstract

Activity of Essential Oils Against Multidrug-Resistant Salmonella enteritidis

et al. 2022

View full text Add to dashboard Cite

Libyan Thymus capitatus essential oil: antioxidant, antimicrobial, cytotoxic and colon pathogen adhesion-inhibition properties

Abstract: This is the first report that EO of Th. capitatus could protect against colonization of pathogens to colon epithelium. Thymus capitatus from Libya should be recognized as possible new source of natural antioxidants, antimicrobials as well as possible source of new chemotherapeutics.

Cited by 45 publications

References 40 publications

Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants

Bioactivity of Juniperus communis essential oil and post-distillation waste: Assessment of selective toxicity against food contaminants

Evaluation of antifungal activity and cytotoxicity of Thymus vulgaris essential oil

Activity of Essential Oils Against Multidrug-Resistant Salmonella enteritidis

Contact Info

Product

Resources

About