Li + activated nanohydroxyapatite doped with Eu 3+ ions enhances proliferative activity and viability of human stem progenitor cells of adipose tissue and olfactory ensheathing cells. Further perspective of nHAP:Li + , Eu 3+ application in theranostics

Marycz, Krzysztof; Sobierajska, Paulina; Śmieszek, Agnieszka; Marędziak, Monika; Wiglusz, Katarzyna; Wiglusz, Rafał J.

doi:10.1016/j.msec.2017.04.041

Cited by 41 publications

(34 citation statements)

References 59 publications

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“…z o.o., Zalesie Gorne, Poland) at 0.5 µm steps up to a final depth of 25 µm. Images were processed using Fiji is just ImageJ (ImageJ 1.52n,Wayne Rasband, National Institute of Health, USA) [27,32]. The microscopic images were obtained using maximum intensity projection (Z-projection).…”

Section: Immunocytochemical Detection Of Osteopontin and Tartrate-resmentioning

confidence: 99%

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

Śmieszek

Marcinkowska

Pielok

et al. 2020

Cells

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MiR-21 is being gradually more and more recognized as a molecule regulating bone tissue homeostasis. However, its function is not fully understood due to the dual role of miR-21 on bone-forming and bone-resorbing cells. In this study, we investigated the impact of miR-21 inhibition on pre-osteoblastic cells differentiation and paracrine signaling towards pre-osteoclasts using indirect co-culture model of mouse pre-osteoblast (MC3T3) and pre-osteoclast (4B12) cell lines. The inhibition of miR-21 in MC3T3 cells (MC3T3inh21) modulated expression of genes encoding osteogenic markers including collagen type I (Coll-1), osteocalcin (Ocl), osteopontin (Opn), and runt-related transcription factor 2 (Runx-2). Inhibition of miR-21 in osteogenic cultures of MC3T3 also inflected the synthesis of OPN protein which is essential for proper mineralization of extracellular matrix (ECM) and anchoring osteoclasts to the bones. Furthermore, it was shown that in osteoblasts miR-21 regulates expression of factors that are vital for survival of pre-osteoclast, such as receptor activator of nuclear factor κB ligand (RANKL). The pre-osteoclast cultured with MC3T3inh21 cells was characterized by lowered expression of several markers associated with osteoclasts’ differentiation, foremost tartrate-resistant acid phosphatase (Trap) but also receptor activator of nuclear factor-κB ligand (Rank), cathepsin K (Ctsk), carbonic anhydrase II (CaII), and matrix metalloproteinase (Mmp-9). Collectively, our data indicate that the inhibition of miR-21 in MC3T3 cells impairs the differentiation and ECM mineralization as well as influences paracrine signaling leading to decreased viability of pre-osteoclasts.

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Section: Immunocytochemical Detection Of Osteopontin and Tartrate-resmentioning

confidence: 99%

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

Śmieszek

Marcinkowska

Pielok

et al. 2020

Cells

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“…38 The emission and excitation of UCNPs is located in the optical window of biological tissue what minimize the overheating effect from the excitation irradiation at 980 nm the biological markers.…”

Section: 37mentioning

confidence: 99%

Preparation of up-converting nano-biphasic calcium phosphate

et al. 2017

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The nano-biphasic calcium phosphate co-doped with 1 mol% Er 3+ and 5 mol% Yb 3+ ions was prepared using Pechini's technique. The preparation of the biphasic calcium phosphate showed the removal of fluorine ions from the matrix. The structural properties and morphology of the particles were studied by means of XRD (X-ray powder diffraction), TEM (Transmission Electron Microscopy), and SEM (Scanning nano-biphasic calcium phosphate were investigated in detail using emission spectra, power dependence and emission kinetics. This material demonstrated intense green up-conversion depending on laser power. Additionally, the luminescence lifetimes for Er 3+ ions were calculated from the measured decays.Power dependence of the green and red emissions was found to result from a temperature increase of nano-biphasic calcium phosphate that opens potential applications in theranostics.

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“…Applications include maxillofacial reconstruction, treatment of periodontal disease as well as bone repair after tumor surgery due to its ability to promote bone ingrowth [ 18 ]. Our previous work showed that synthetic nanocrystalline hydroxyapatite has proper biocompatibility, even after structural modification by ionic substitutions [ 19 ]. The biological activity of nanoapatite (as well as other nanoparticles) results from its surface chemistry associated with high surface area to volume ratio.…”

Section: Introductionmentioning

confidence: 99%

Nanohydroxyapatite-Mediated Imatinib Delivery for Specific Anticancer Applications

et al. 2020

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In the present study, a nanoapatite-mediated delivery system for imatinib has been proposed. Nanohydroxyapatite (nHAp) was obtained by co-precipitation method, and its physicochemical properties in combination with imatinib (IM) were studied by means of XRPD (X-ray Powder Diffraction), SEM-EDS (Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy), FT-IR (Fourier-Transform Infrared Spectroscopy), absorption spectroscopy as well as DLS (Dynamic Light Scattering) techniques. The obtained hydroxyapatite was defined as nanosized rod-shaped particles with high crystallinity. The amorphous imatinib was obtained by conversion of its crystalline form. The beneficial effects of amorphous pharmaceutical agents have been manifested in the higher dissolution rate in body fluids improving their bioavailability. Imatinib-to-hydroxyapatite interactions on the surface were confirmed by SEM images as well as absorption and FT-IR spectroscopy. The cytotoxicity of the system was tested on NI-1, L929, and D17 cell lines. The effectiveness of imatinib was not affected by nHAp modification. The calculated IC50 values for drug-modified nHAp were similar to those for the drug itself. However, higher cytotoxicity was observed at higher concentrations of imatinib, in comparison with the drug alone.

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Li + activated nanohydroxyapatite doped with Eu 3+ ions enhances proliferative activity and viability of human stem progenitor cells of adipose tissue and olfactory ensheathing cells. Further perspective of nHAP:Li + , Eu 3+ application in theranostics

Cited by 41 publications

References 59 publications

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

The Role of miR-21 in Osteoblasts–Osteoclasts Coupling In Vitro

Preparation of up-converting nano-biphasic calcium phosphate

Nanohydroxyapatite-Mediated Imatinib Delivery for Specific Anticancer Applications

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