LHY and CCA1 Are Partially Redundant Genes Required to Maintain Circadian Rhythms in Arabidopsis

Mizoguchi, Tsuyoshi; Wheatley, Kay; Hanzawa, Yoshie; Wright, Louisa; Mizoguchi, Mutsuko; Song, Hae Ryong; Carré, Isabelle A.; Coupland, George

doi:10.1016/s1534-5807(02)00170-3

Cited by 560 publications

(705 citation statements)

References 44 publications

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“…1F and 1G). These results were consistent with previously reported data (Alonso et al, 2003;Mizoguchi et al, 2002;Somers et al, 1998), suggesting that our imaging system is in fact an accurate method for screening leaf movement mutants.…”

Section: Resultssupporting

confidence: 93%

A Genetic Screen for Leaf Movement Mutants Identifies a Potential Role for AGAMOUS-LIKE 6 (AGL6) in Circadian-Clock Control

Yoo

Hong²,

Lee

et al. 2011

Molecules and Cells

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The circadian clock in plants regulates many important physiological and biological processes, including leaf movement. We have used an imaging system to genetically screen Arabidopsis seedlings for altered leaf movement with the aim of identifying a circadian clock gene. A total of 285 genes were selected from publicly available microarrays that showed an expression pattern similar to those of the Arabidopsis core oscillator genes. We subsequently isolated 42 homozygous recessive mutants and analyzed their leaf movements. We also analyzed leaf movements of activation tagging mutants that showed altered flowering time. We found that agl6-1D plants, in which AGAMOUS-LIKE 6 (AGL6) was activated by the 35S enhancer, showed a shortened period of leaf movement as well as a high level of ZEITLUPE (ZTL) expression, reduced amplitude of LATE ELONGATED HYPOCOTYL (LHY) expression, and arrhythmic TIMING OF CAB EXPRES-SION1 (TOC1)/CIRCADIAN CLOCK ASSOCIATED1 (CCA1) expression. A shortened period of leaf movement was also seen in 35S-AGL6-myc plants, although 35S-amiRAGL6 plants, transgenic plants overexpressing an artificial miRNA (amiR) targeting AGL6, showed unaltered leaf movement. The amplitude of CHLOROPHYLL A/B BINDING PROTEIN 2 (CAB2) expression, a circadian output gene, was also reduced in agl6-1D plants. Taken together, these results suggest that AGL6 plays a potential role in the regulation of the circadian clock by regulating ZTL mRNA level in Arabidopsis.

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Section: Resultssupporting

confidence: 93%

A Genetic Screen for Leaf Movement Mutants Identifies a Potential Role for AGAMOUS-LIKE 6 (AGL6) in Circadian-Clock Control

Yoo

Hong²,

Lee

et al. 2011

Molecules and Cells

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“…CCA1 and LHY bind to an EE in the TOC1 promoter, repressing its expression as part of the mechanism by which the circadian oscillator functions (Fig. 6;Harmer et al, 2000;Mizoguchi et al, 2002). Our searches for promoter sequences revealed three EE in the ELF4 promoter ( Fig.…”

Section: Discussionmentioning

confidence: 82%

“…CCA1, LHY, and TOC1, which is a pseudoresponse regulator, function in an autoregulatory feedback loop and are integral components of the central oscillator in Arabidopsis (Harmer et al, 2001;Devlin, 2002). The evening element (EE; AAATATCT) is a known binding site for the CCA1 and LHY proteins in the TOC1 promoter, repressing its expression (Harmer et al, 2000;Mizoguchi et al, 2002). The transcriptional feedback loop is completed by TOC1 activating the expression of CCA1 and LHY late in the day (Alabadi et al, 2001).…”

mentioning

confidence: 99%

EARLY FLOWERING 4Functions in Phytochrome B-Regulated Seedling De-Etiolation

Khanna

Kikis²,

Quail³

2003

Plant Physiology

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To define the functions of genes previously identified by expression profiling as being rapidly light induced under phytochrome (phy) control, we are investigating the seedling de-etiolation phenotypes of mutants carrying T-DNA insertional disruptions at these loci. Mutants at one such locus displayed reduced responsiveness to continuous red, but not continuous far-red light, suggesting a role in phyB signaling but not phyA signaling. Consistent with such a role, expression of this gene is induced by continuous red light in wild-type seedlings, but the level of induction is strongly reduced in phyB-null mutants. The locus encodes a novel protein that we show localizes to the nucleus, thus suggesting a function in light-regulated gene expression. Recently, this locus was identified as EARLY FLOWERING 4, a gene implicated in floral induction and regulating the expression of the gene CIRCADIAN CLOCK-ASSOCIATED 1. Together with these previous data, our findings suggest that EARLY FLOWERING 4 functions as a signaling intermediate in phy-regulated gene expression involved in promotion of seedling de-etiolation, circadian clock function, and photoperiod perception.

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“…S11). For example, RA1 and KN1 co-occupy the promoter of a MYB TF orthologous to LATE ELONGATED HYPOCOTYL/CIRCADIAN CLOCK ASSOCIATED1 (LHY/CCA1), and the intron of bZIP TF liguleless2 (lg2), two genes that function in the transition from vegetative to reproductive development (Walsh et al 1998;Mizoguchi et al 2002). Although ra1 is expressed after the transition to inflorescence development, this binding could reveal feedback regulation of the transition.…”

Section: Common Targets Of Ra1 and Knotted1 Link Meristem Determinacymentioning

confidence: 99%

Regulatory modules controlling maize inflorescence architecture

et al. 2013

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Genetic control of branching is a primary determinant of yield, regulating seed number and harvesting ability, yet little is known about the molecular networks that shape grain-bearing inflorescences of cereal crops. Here, we used the maize (Zea mays) inflorescence to investigate gene networks that modulate determinacy, specifically the decision to allow branch growth. We characterized developmental transitions by associating spatiotemporal expression profiles with morphological changes resulting from genetic perturbations that disrupt steps in a pathway controlling branching. Developmental dynamics of genes targeted in vivo by the transcription factor RAMOSA1, a key regulator of determinacy, revealed potential mechanisms for repressing branches in distinct stem cell populations, including interactions with KNOTTED1, a master regulator of stem cell maintenance. Our results uncover discrete developmental modules that function in determining grass-specific morphology and provide a basis for targeted crop improvement and translation to other cereal crops with comparable inflorescence architectures.

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LHY and CCA1 Are Partially Redundant Genes Required to Maintain Circadian Rhythms in Arabidopsis

Cited by 560 publications

References 44 publications

A Genetic Screen for Leaf Movement Mutants Identifies a Potential Role for AGAMOUS-LIKE 6 (AGL6) in Circadian-Clock Control

A Genetic Screen for Leaf Movement Mutants Identifies a Potential Role for AGAMOUS-LIKE 6 (AGL6) in Circadian-Clock Control

EARLY FLOWERING 4Functions in Phytochrome B-Regulated Seedling De-Etiolation

Regulatory modules controlling maize inflorescence architecture

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