LHX6 is essential for the migration of human pluripotent stem cell-derived GABAergic interneurons

Fang, Yuan; Fang, Kai-Heng; Hong, Yuan; Xu, Shi-Bo; Xu, Min; Pan, Yufeng; Liu, Yan

doi:10.1007/s13238-019-00686-6

Cited by 14 publications

(11 citation statements)

References 14 publications

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“…S8a ). In particular, some highly conserved TFs such as the regulator of neural stem cells tcf4 51 and the known neural developmental regulator lhx6 52 were detected in four species (Fig. S8a ).…”

Section: Resultsmentioning

confidence: 99%

Cell landscape of larval and adult Xenopus laevis at single-cell resolution

Yuan

Guo

et al. 2022

Nat Commun

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The rapid development of high-throughput single-cell RNA sequencing technology offers a good opportunity to dissect cell heterogeneity of animals. A large number of organism-wide single-cell atlases have been constructed for vertebrates such as Homo sapiens, Macaca fascicularis, Mus musculus and Danio rerio. However, an intermediate taxon that links mammals to vertebrates of more ancient origin is still lacking. Here, we construct the first Xenopus cell landscape to date, including larval and adult organs. Common cell lineage-specific transcription factors have been identified in vertebrates, including fish, amphibians and mammals. The comparison of larval and adult erythrocytes identifies stage-specific hemoglobin subtypes, as well as a common type of cluster containing both larval and adult hemoglobin, mainly at NF59. In addition, cell lineages originating from all three layers exhibits both antigen processing and presentation during metamorphosis, indicating a common regulatory mechanism during metamorphosis. Overall, our study provides a large-scale resource for research on Xenopus metamorphosis and adult organs.

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Section: Resultsmentioning

confidence: 99%

Cell landscape of larval and adult Xenopus laevis at single-cell resolution

Yuan

Guo

et al. 2022

Nat Commun

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“…Brain assembloids include 1) fused region-specific brain organoids, 2) region-specific brain organoids co-cultured with other non-neuronal but physiologically relevant cells/tissues and finally 3) brain organoids co-cultured with organizers that releases a gradient of extrinsic signaling to mimic the dorsal-ventral and anterior-posterior axis determinants of the fetal brain. Many studies have fused relevant region-specific brain organoids to study interneuron migration ( 44 , 45 , 49 , 62 ), neuronal projections and oligodendrogenesis ( 53 ). Methods to create region-specific brain organoids with vasculature for their better survival have been reported ( 63 - 65 ).…”

Section: Region Specific Brain Organoidsmentioning

confidence: 99%

Region Specific Brain Organoids to Study Neurodevelopmental Disorders

Susaimanickam

Kiral

Park

2022

IJSC

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Region specific brain organoids are brain organoids derived by patterning protocols using extrinsic signals as opposed to cerebral organoids obtained by self-patterning. The main focus of this review is to discuss various region-specific brain organoids developed so far and their application in modeling neurodevelopmental disease. We first discuss the principles of neural axis formation by series of growth factors, such as SHH, WNT, BMP signalings, that are critical to generate various region-specific brain organoids. Then we discuss various neurodevelopmental disorders modeled so far with these region-specific brain organoids, and findings made on mechanism and treatment options for neurodevelopmental disorders (NDD).

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“…Furthermore, using a dorsoventral organoid fusion model, Yuan et al ( 2020 ) showed that LHX6 is essential for GABAergic interneuron migration. Another study implanted patient iPSC-derived ventralized organoids into the mouse brain to investigate the role of OLIG2 in Down syndrome etiology (Xu et al, 2019 ).…”

Section: Interneuron Migrationmentioning

confidence: 99%

Application of Fused Organoid Models to Study Human Brain Development and Neural Disorders

Chen

Guo

Fang

et al. 2020

Front. Cell. Neurosci.

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Human brain organoids cultured from human pluripotent stem cells provide a promising platform to recapitulate histological features of the human brain and model neural disorders. However, unlike animal models, brain organoids lack a reproducible topographic organization, which limits their application in modeling intricate biology, such as the interaction between different brain regions. To overcome these drawbacks, brain organoids have been pre-patterned into specific brain regions and fused to form an assembloid that represents reproducible models recapitulating more complex biological processes of human brain development and neurological diseases. This approach has been applied to model interneuron migration, neuronal projections, tumor invasion, oligodendrogenesis, forebrain axis establishment, and brain vascularization. In this review article, we will summarize the usage of this technology to understand the fundamental biology underpinning human brain development and disorders.

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LHX6 is essential for the migration of human pluripotent stem cell-derived GABAergic interneurons

Cited by 14 publications

References 14 publications

Cell landscape of larval and adult Xenopus laevis at single-cell resolution

Cell landscape of larval and adult Xenopus laevis at single-cell resolution

Region Specific Brain Organoids to Study Neurodevelopmental Disorders

Application of Fused Organoid Models to Study Human Brain Development and Neural Disorders

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