LH surge in response to the treatment with GnRH analog or estradiol in ovariectomized buffaloes with or without progesterone pre-exposition

Different times to perform timed artificial insemination when using a P4/E2/eCG-based protocol in buffalo

“…Other evidence has confirmed EB treatment efficiency for the induction of ovulation in buffalo. Studies have shown that EB treatment induces considerable release of LH (JACOMINI et al, 2014), has a low cost (BARROS et al, 2000;MANES et al, 2012) and maintains high circulating oestradiol concentrations, which likely creates a better uterine environment for embryonic development (BRIDGES et al, 2012).…”

>b<Factors that affect the embryo production and fertility of buffalo donors and recipientes>/b<

“…The gonadotrophin releasing hormone (GnRH) is a decapeptide synthesized by the hypothalamus, which acts on the pituitary and stimulates to the release of the hormones LH (luteinizing hormone) and FSH (follicle stimulating hormone). The administration of GnRH and GnRH agonists induces an acute release of gonadotrophins in cattle (MARTINEZ et al, 2003) and buffaloes (BARUSELLI et al, 2003;RASTEGARNIA et al, 2004;JACOMINI et al, 2014) and produces ovulation in follicles that are at the appropriate maturation stage and have the relevant LH receptors (XU et al, 1995). In buffaloes, treatment with GnRH has proved to be effective in inducing ovulation, accompanied by the appearance of luteal tissue in the ovary within 14 h of ovulation, an increase in circulating concentrations of progesterone within 96 hours (RASTEGARNIA et al, 2004) and the formation of a CL accessory (CAMPANILE et al, 2007a(CAMPANILE et al, , 2008CARVALHO et al, 2007).…”