LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian

Morsy, Heba; Gaballah, Ahmed; Samir, Mohamed; Nakundi, Vandrome; Shamseya, Mohamed; Mahrous, Hanan; Ghazal, Abeer; Hashish, Mervat M.; Arafat, Waleed

doi:10.31557/apjcp.2021.22.9.2819

Cited by 1 publication

(1 citation statement)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Due to its key role in tumorigenesis, LGR5 expression has shown a significant correlation with the survival of patients according to many previously published studies which explored unfavorable prognostic results in CRC [ 45 , 52 , 53 , 54 ]. However, contrary results have been suggested by certain reports for the correlation between LGR5 staining scores and the prognosis of CRC [ 5 ], for example, a genetic study published in 2012 by Ziskin et al [ 55 ] and an immunohistochemical study published in 2019 by Shekarriz et al [ 56 ] reported that LGR5 expression was not seen significantly associated with the aggressiveness of colorectal tumors.…”

Section: Discussionmentioning

confidence: 99%

The Expression of Stem Cell Marker LGR5 and Its Coexpression with Β-Catenin in Sporadic Colorectal Carcinoma and Adenoma: A Comparative Immunohistochemical Study

Ahmed,

Farag,

Abdelwahed

et al. 2023

Medicina

View full text Add to dashboard Cite

Background: LGR5 is one of the most important stem cell markers for colorectal cancer (CRC), as it potentiates Wnt/Β-catenin signaling. The well-characterized deregulation of Wnt/Β-catenin signaling that occurs during adenoma/carcinoma sequence in CRC renders LGR5 a hopeful therapeutic target. We assessed the immunohistochemical expression of LGR5 and Β-catenin in normal colonic and tumorous lesions with a clinicopathological correlation. Methods: Tissue blocks and clinical data of 50 selected cases were included: 8 from normal mucosa, 12 cases of adenoma, and 30 cases of CRC, where sections were cut and re-examined and the immunohistochemical technique was conducted using anti-LGR5 and anti-Β-catenin to measure the staining density. Results: There was no expression of LGR5 in normal mucosa compared to samples of adenoma and CRC samples. The association analysis showed that CRC specimens were more likely to have strong LGR5 and Β-catenin expressions than the other two groups (p = 0.048 and p < 0.001, respectively). Specimens with high-grade dysplastic adenoma were more likely to express moderate-to-strong expression of LGR5 and Β-catenin (p = 0.013 and p = 0.036, respectively). In contrast, there were no statistically significant associations between LGR5 and Β-catenin expression with grade and stage. Conclusion: These results suggest and support the possible role of LGR5 as a potential marker of cancer stem cells in sporadic colorectal carcinogenesis in addition to a prognostic value for LGR5 and Β-catenin in adenomatous lesions according to immunohistochemical expression density. A potential therapeutic role of LGR5 in CRC is suggested for future studies based on its role in pathogenesis.

show abstract