LGL-1: A Nonpolymorphic Antigen Expressed on a Major Population of Mouse Natural Killer Cells

Mason, Llewellyn H.; Giardina, Steven L.; Hecht, Toby T.; Ortaldo, John R.; Mathieson, Bonnie J.

doi:10.1159/000416288

Cited by 45 publications

(43 citation statements)

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“…62 Briefly, single cell suspensions were prepared by passing the spleens through a wire mesh screen. RBC were lysed with ACK reagent (Biowhittiker, Walkerville, MD, USA) and cells were washed and resuspended in 5% fetal bovine serum (FBS) RPMI 1640.…”

Section: Nk Cell Isolationmentioning

confidence: 99%

Stat4-dependent, T-bet-independent regulation of IL-10 in NK cells

Yao

Hedrich

et al. 2008

Genes Immun

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Section: Nk Cell Isolationmentioning

confidence: 99%

Stat4-dependent, T-bet-independent regulation of IL-10 in NK cells

Yao

Hedrich

et al. 2008

Genes Immun

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“…Primary murine NK cells were prepared and cultured for 4 -8 days in recombinant human IL-2 (Chiron, Emoryville, CA) as described (35). In some cases, mice were pretreated with IL-2 to increase NK cell infiltration into the liver.…”

Section: Abs Nk Cells and Cell Linesmentioning

confidence: 99%

Aberrant DAP12 Signaling in the 129 Strain of Mice: Implications for the Analysis of Gene-Targeted Mice

McVicar

Winkler‐Pickett

Taylor

et al. 2002

The Journal of Immunology

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NK cells are implicated in antiviral responses, bone marrow transplantation and tumor immunosurveillance. Their function is controlled, in part, through the Ly49 family of class I binding receptors. Inhibitory Ly49s suppress signaling, while activating Ly49s (i.e., Ly49D) activate NK cells via the DAP12 signaling chain. Activating Ly49 signaling has been studied primarily in C57BL/6 mice, however, 129 substrains are commonly used in gene-targeting experiments. In this study, we show that in contrast to C57BL/6 NK cells, cross-linking of DAP12-coupled receptors in 129/J mice induces phosphorylation of DAP12 but not calcium mobilization or cytokine production. Consistent with poor-activating Ly49 function, 129/J mice reject bone marrow less efficiently than C57BL/6 mice. Sequence analysis of receptors and DAP12 suggests no structural basis for inactivity, and both the 129/J and C57BL/6 receptors demonstrate normal function in a reconstituted receptor system. Most importantly, reconstitution of Ly49D in 129/J NK cells demonstrated that the signaling deficit is within the NK cells themselves. These unexpected findings bring into question any NK analysis of 129/J, 129Sv, or gene-targeted mice derived from these strains before complete backcrossing, and provide a possible explanation for the differences observed in the immune response of 129 mice in a variety of models.

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“…The purity of human and mouse CD3 ϩ cell preparations as confirmed by flow cytometry was routinely Ͼ95%. Enriched mouse NK cells were obtained from spleen cell preparations as described previously (21). The resulting preparation routinely contained Ͼ75% DX5 ϩ , CD3 Ϫ NK cells as assessed by flow cytometry.…”

Section: Purification Of Cd3 ϩ T Cells or Dx5 ϩ Nk Cellsmentioning

confidence: 99%

Primary Hepatocytes from Mice Treated with IL-2/IL-12 Produce T Cell Chemoattractant Activity that Is Dependent on Monokine Induced by IFN-γ (Mig) and Chemokine Responsive to γ-2 (Crg-2)

Park

Gruys

McCormick³

et al. 2001

The Journal of Immunology

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The IFN-γ-inducible proteins monokine induced by IFN-γ (Mig) and chemokine responsive to γ-2 (Crg-2) can contribute to IL-12-induced antiangiogenic and leukocyte-recruiting activities, but the extent to which leukocytes vs parenchymal cells in different organs contribute to the production of these molecules remains unclear. The results presented herein show that IFN-γ-dependent induction of Mig and Crg-2 gene expression can occur in many nonlymphoid organs, and these genes are rapidly induced in purified hepatocytes isolated from mice treated with IL-2 plus IL-12, or from Hepa 1-6 hepatoma cells treated in vitro with IFN-γ. In addition to depending on IFN-γ, the ability of IL-12 or IL-2/IL-12 to induce Mig and Crg-2 gene expression in purified hepatocytes also is accompanied by the coordinate up-regulation of the IFN-γ R α and β-chains, in the absence of IL-12R components. Supernatants of primary hepatocytes obtained from mice treated in vivo with IL-2/IL-12 or from hepatocytes treated in vitro with IFN-γ contain increased chemotactic activity for enriched human and mouse CD3+ T cells, as well as mouse DX5+ NK cells. The hepatocyte-derived chemotactic activity for mouse T cells but not NK cells was ablated by Abs specific for Mig and Crg-2. These results suggest that parenchymal cells in some organs may contribute substantially to initiation and/or amplification of inflammatory or antitumor responses.

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LGL-1: A Nonpolymorphic Antigen Expressed on a Major Population of Mouse Natural Killer Cells

Cited by 45 publications

References 0 publications

Stat4-dependent, T-bet-independent regulation of IL-10 in NK cells

Stat4-dependent, T-bet-independent regulation of IL-10 in NK cells

Aberrant DAP12 Signaling in the 129 Strain of Mice: Implications for the Analysis of Gene-Targeted Mice

Primary Hepatocytes from Mice Treated with IL-2/IL-12 Produce T Cell Chemoattractant Activity that Is Dependent on Monokine Induced by IFN-γ (Mig) and Chemokine Responsive to γ-2 (Crg-2)

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