Levothyroxin Inhibits Heterotopic Ossification: An Experimental Study in Rabbits

Namazi, Hamid; Mozaffarian, Kamran

doi:10.1097/ta.0b013e3181581f81

Cited by 3 publications

(10 citation statements)

References 20 publications

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“…Most of these models have variable repeatability, but the implantation of bone marrow to induce heterotopic bone formation can be demonstrated simply without the demand of time needed to prepare the material to be deployed. The induction procedure used in the present study was adopted from studies (5,10,11) that have documented that 35 days are sufficient for heterotopic bone formation in the quadriceps of rabbits following implantation of 2 mL BM collected from the iliac crest of the animal. Namazi and Mozaffarian (5) observed that after 35 days of BM implant in rabbits, heterotopic bone formation occurred in 83.3% of control animals.…”

Section: Discussionmentioning

confidence: 99%

“…The induction procedure used in the present study was adopted from studies (5,10,11) that have documented that 35 days are sufficient for heterotopic bone formation in the quadriceps of rabbits following implantation of 2 mL BM collected from the iliac crest of the animal. Namazi and Mozaffarian (5) observed that after 35 days of BM implant in rabbits, heterotopic bone formation occurred in 83.3% of control animals. Dudkiewicz et al (11), using the same methodology for HO induction, observed 97% heterotopic bone formation.…”

Section: Discussionmentioning

confidence: 99%

“…The minimum implant volume was adopted from published studies (5,11). The maximum volume of BM was determined as the maximum volume supported by the animal as long as there was no leakage of liquid during the implant.…”

Section: Methodsmentioning

confidence: 99%

“…The animals were euthanized 35 days after BM implantation, a period considered to be sufficient for the development of heterotopic bone formation (5,11). …”

Section: Methodsmentioning

confidence: 99%

“…It is associated with spinal cord and brain injuries, burns, fractures, muscle contusion, joint arthroplasty, lower motor neuron disorders, and hereditary disorders (1,5-7). HO induction was first reported in the 1930s (8,9), and since then animal models have been used in different forms of HO induction in order to define techniques that may aid in the prevention and treatment of the condition.…”

Section: Introductionmentioning

confidence: 99%

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Experimental model of heterotopic ossification in Wistar rats

Zotz

Paula

Moser

2012

Braz J Med Biol Res

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Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues adjacent to large joints, resulting in joint mobility deficit. In order to determine which treatment techniques are more appropriate for such condition, experimental models of induced heterotopic bone formation have been proposed using heterologous demineralized bone matrix implants and bone morphogenetic protein and other tissues. The objective of the present experimental study was to identify a reliable protocol to induce HO in Wistar rats, based on autologous bone marrow (BM) implantation, comparing 3 different BM volumes and based on literature evidence of this HO induction model in larger laboratory animals. Twelve male Wistar albino rats weighing 350/390 g were used. The animals were anesthetized for blood sampling before HO induction in order to quantify serum alkaline phosphatase (ALP). HO was induced by BM implantation in both quadriceps muscles of these animals, experimental group (EG). Thirty-five days after the induction, another blood sample was collected for ALP determination. The results showed a weight gain in the EG and no significant difference in ALP levels when comparing the periods before and after induction. Qualitative histological analysis confirmed the occurrence of heterotopic ossification in all 12 EG rats. In conclusion, the HO induction model was effective when 0.35 mL autologous BM was applied to the quadriceps of Wistar rats.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The animals were euthanized 35 days after BM implantation, a period considered to be sufficient for the development of heterotopic bone formation (5,11). …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Experimental model of heterotopic ossification in Wistar rats

Zotz

Paula

Moser

2012

Braz J Med Biol Res

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Heterotopic ossification following cardiac arrest and hypoxic brain damage

Mackiewicz−Milewska

Jung

Lach-Inszczak

et al. 2010

International Journal of Therapy and Rehabilitation

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Aims Heterotopic bone can cause restriction of movement when deposited in the soft tissues around joints. Neurogenic heterotopic ossification may follow brain injury. This article describes research into the incidence of heterotopic ossification after brain injury, and its impact on rehabilitation. Methods Thirty-nine patients who were admitted to the rehabilitation unit of University Hospital Bydgoszcz, Poland, after hypoxic brain injury due to cardiac arrest, were examined. Findings Six patients were identified as having developed heterotopic bone around one or more joints causing limitation of movement and delay in the rehabilitation process. Conclusions Heterotopic ossification occurs in up to 15% of patients following hypoxic brain injury. The development of the condition may often be missed clinically, and can hinder rehabilitation.

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Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats

Zotz

Paula

2015

Braz J Med Biol Res

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Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues, resulting in joint mobility deficit and pain. Different treatment modalities have been tried to prevent HO development, but there is no consensus on a therapeutic approach. Since electrical stimulation is a widely used resource in physiotherapy practice to stimulate joint mobility, with analgesic and anti-inflammatory effects, its usefulness for HO treatment was investigated. We aimed to identify the influence of electrical stimulation on induced HO in Wistar rats. Thirty-six male rats (350-390 g) were used, and all animals were anesthetized for blood sampling before HO induction, to quantify the serum alkaline phosphatase. HO induction was performed by bone marrow implantation in both quadriceps of the animals, which were then divided into 3 groups: control (CG), transcutaneous electrical nerve stimulation (TENS) group (TG), and functional electrical stimulation (FES) group (FG) with 12 rats each. All animals were anesthetized and electrically stimulated twice per week, for 35 days from induction day. After this period, another blood sample was collected and quadriceps muscles were bilaterally removed for histological and calcium analysis and the rats were killed. Calcium levels in muscles showed significantly lower results when comparing TG and FG (P<0.001) and between TG and CG (P<0.001). Qualitative histological analyses confirmed 100% HO in FG and CG, while in TG the HO was detected in 54.5% of the animals. The effects of the muscle contractions caused by FES increased HO, while anti-inflammatory effects of TENS reduced HO.

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Levothyroxin Inhibits Heterotopic Ossification: An Experimental Study in Rabbits

Abstract: Levothyroxin demonstrated efficacy in preventing heterotopic ossification.

Cited by 3 publications

References 20 publications

Experimental model of heterotopic ossification in Wistar rats

Experimental model of heterotopic ossification in Wistar rats

Heterotopic ossification following cardiac arrest and hypoxic brain damage

Influence of transcutaneous electrical stimulation on heterotopic ossification: an experimental study in Wistar rats

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