Leveraging Pseudomonas Stress Response Mechanisms for Industrial Applications

Abstract

Members of the genus Pseudomonas are metabolically versatile and capable of adapting to a wide variety of environments. Stress physiology of Pseudomonas strains has been extensively studied because of their biotechnological potential in agriculture as well as their medical importance with regards to pathogenicity and antibiotic resistance. This versatility and scientific relevance led to a substantial amount of information regarding the stress response of a diverse set of species such as Pseudomonas chlororaphis, P. fluorescens, P. putida, P. aeruginosa, and P. syringae. In this review, environmental and industrial stressors including desiccation, heat, and cold stress, are cataloged along with their corresponding mechanisms of survival in Pseudomonas. Mechanisms of survival are grouped by the type of inducing stress with a focus on adaptations such as synthesis of protective substances, biofilm formation, entering a non-culturable state, enlisting chaperones, transcription and translation regulation, and altering membrane composition. The strategies Pseudomonas strains utilize for survival can be leveraged during the development of beneficial strains to increase viability and product efficacy.

Monochloramine Induces Release of DNA and RNA from Bacterial Cells: Quantification, Sequencing Analyses, and Implications

“…These genes are generally induced in response to the improper folding of proteins and their aggregation. 67 Transcripts for the spy chaperone were also detected in high quantities. Spy is known to express only under cell envelope stress conditions and aids in the proper folding of the proteins in the periplasmic space.…”

“…Transcripts for several chaperones ( groEL / groES , dnaK , gprE , clpB , and htpG ) and proteolytic systems ( clpA , clpS , clpX , and degP ) were also detected in the emRNA. These genes are generally induced in response to the improper folding of proteins and their aggregation . Transcripts for the spy chaperone were also detected in high quantities.…”

Pseudomonas: versatile biocatalysts for PFAS

“…Of the 16 fluorinated compounds in the former database, 11 are involved in reactions known to be carried out by Pseudomonas species. Publications describing the general metabolic versatility, survivability and biodegradative capacities of Pseudomonas species are far too many to cite here; fortunately, numerous excellent reviews are available (Ramos et al ., 1994; Shintani et al ., 2010; Akkaya et al ., 2018; Molina et al ., 2020; Craig et al ., 2021; Nikel and de Lorenzo, 2021).…”

Self-Assembled Nanocoatings Protect Microbial Fertilizers for Climate-Resilient Agriculture

“…[29][30][31] It can also protect plants against phytopathogenic fungi and pests. 32,33 However, P. chlororaphis is highly unstable at elevated temperatures, and prior efforts to protect this species have failed. 34 We demonstrate the protection of P. chlororaphis with MPNs.…”