Levels of total antioxidant capacity and 8-hydroxy-2′-deoxyguanosine of serum and follicular fluid in women undergoing <i>in vitro</i> fertilization: focusing on endometriosis

Várnagy, Ákos; Kőszegi, Tamás; Györgyi, Erzséber; Szegedi, Sarolta; Sulyok, E.; Prémusz, Viktória; Bódis, József

doi:10.1080/14647273.2018.1535719

Cited by 26 publications

(29 citation statements)

References 34 publications

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“…However, when the models controlled for hormonal factors TSH and Prl, high CAT levels in follicular fluid were still associated with a reduced risk of both poor fertilization rate (≤50%) and unsuccessful live birth. In contrast to the findings of Oyawoye et al (2003) and Varnagy et al (2018), we also observed an association between high levels of follicular TAC and a low probability of poor fertilization rate.…”

Section: Impact Of Oxidative Stress and Dna Damage On Ivf Outcomescontrasting

confidence: 99%

“…Several studies have measured 8-OHdG levels in blood and urine to understand the role of oxidative DNA damage in disease pathogenesis (Evans et al, 2004). To date, very few studies have reported that elevated 8-OHdG levels in follicular fluid are associated with poor oocyte quality (Da Broi et al, 2016, Tamura et al, 2008, Várnagy et al, 2020. The mean value for follicular 8-OHdG levels in this study was 22.40, which were much higher than those reported by Tamura et al (2014) (Vatannejad et al, 2017, Micillo et al, 2016, Tang et al, 2016, Cambi et al, 2013, Nakamura et al, 2002, Sakamoto et al, 2008 and poor assisted reproductive technology (ART) outcome (Ahelik et al, 2015).…”

Section: Oxidative Stress Profile In Couplesmentioning

confidence: 99%

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Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

Al‐Saleh

Coşkun

Al-Rouqi

et al. 2021

Reproduction and Fertility

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This study examined the status of oxidative stress in 599 couples undertaking in vitro fertilization (IVF) treatment and its association with reproductive hormones, smoking, and outcomes. Oxidative stress biomarkers such as malondialdehyde, 8-hydroxy-2-deoxyguanosine, hydrogen peroxide (H2O2), catalase (CAT), and total antioxidant capacity (TAC) were determined in follicular fluid and seminal plasma. Tail moment (TM) was used to evaluate DNA damage in the sperm and granulosa cells. Reproductive hormones in serum and cotinine (COT) in urine, follicular fluid, and seminal plasma samples were determined. Separate multivariate linear regression was used to assess associations between levels of each oxidative stress biomarker and each hormone and smoking parameter (modeled as natural log-transformed). The findings indicate that some oxidative stress and DNA damage biomarkers played a role in disrupting certain reproductive hormones in women and their male partners either by overproducing reactive oxygen species or reducing antioxidant defense capacity. Although women were nonsmokers, COT levels > 50 and 10 µg/L in urine and follicular were observed in 5.7 and 1.7%, respectively. Levels of follicular fluid COT were positively associated with H2O2 and TM. We used log-binomial multivariate regression to estimate relative risks for the association between oxidative stress/DNA damage and IVF binary outcomes (fertilization rate > 50%, biochemical pregnancy, clinical pregnancy, and live birth). An increase in the CAT levels of follicular fluid was associated with a 48 and 41% decrease in the risk of poor fertilization rate (≤50%) and unsuccessful live birth, respectively. After the models were adjusted for hormonal factors, the associations remained the same, except that the elevated TAC in follicular fluid became significantly associated with a decrease of 42% in the risk of poor fertilization rate (≤50%). The higher antioxidant activity (CAT and TAC) in follicular fluid might positively impact specific IVF outcomes. Lay summary Oxidative stress occurs when antioxidant molecules are insufficient in the body to destroy free radicals that can damage the cells, proteins and DNA, causing different health conditions, including infertility. The role of oxidative stress in female infertility has not received as much attention as male infertility, and research is still limited. This study explored whether the overproduction of free radicals can impact the success of in vitro fertilization (IVF) treatment using several biological markers such as hydrogen peroxide, catalase, and total antioxidant capacity. Our findings revealed that the high antioxidant levels in the fluid surrounding the egg were linked with a high fertilization rate. Additionally, oxidative stress status in couples was associated negatively with several reproductive hormones and smoking status. Biomarkers of oxidative stress and DNA damage might have potential applications in evaluating IVF patients’ clinical characteristics such as causes of infertility, hormonal profile, fertilization rate, implantation and live birth.

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Section: Impact Of Oxidative Stress and Dna Damage On Ivf Outcomescontrasting

confidence: 99%

Section: Oxidative Stress Profile In Couplesmentioning

confidence: 99%

Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

Al‐Saleh

Coşkun

Al-Rouqi

et al. 2021

Reproduction and Fertility

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“…A more recent study of OS in endometriosis patients led to results similar to ours. 68 The concentration of 8-OHdG was similar in the control group to that of patients with endometriosis. But in both studies, all of the follicular fluid was used, not only from the leading follicle.…”

Section: Discussionmentioning

confidence: 65%

“…The evaluation of the pathophysiology of a couple's infertility has shown that oxidative stress (OS) may be one of the causative factors of female infertility, as recent studies shown. 11,28,[51][52][53] But so far there is still a big gap in our knowledge and understanding of individual mechanisms, and further research is needed to be able to use the acquired knowledge to improve the outcome of IVF procedures. Many degenerative changes to the oocytes during ageing are due to oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Antimüllerian Hormone and Oxidative Stress Biomarkers as Predictors of Successful Pregnancy in Polycystic Ovary Syndrome, Endometriosis and Tubal Infertility Factor

Fabjan¹,

VrtaÄŤnik-Bokal²,

Virant‐Klun³

et al. 2020

ACSi

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Oxidative stress in the follicular fluid (FF) is thought to be responsible for the abnormal development of oocytes. In our study patients with polycystic ovarian syndrome (PCOS), endometriosis, and tubal infertility factor (TIF), and healthy women with a male factor of infertility, were prospectively enrolled. From each patient, a sample of individual FF was collected from a dominant follicle. Concentration levels of TAS, 8-IP, 8-OHdG, and AMH were determined. In women with PCOS, we found significantly lower values of oxidative stress markers in the FF. 8-IP and TAS levels were lower in the FF of women with endometriosis. In women with TIF, we also found significantly lower values of all tested markers in the FF, except for 8-OHdG and AMH. We wanted to see whether the biomarker measured in the FF in an individual diagnosis could predict a successfully obtained embryo from this particular follicle. The FF 8-OHdG result in PCOS patients stood out and proved to be a good predictive marker of matured and fertilized oocytes in these patients. Further research is needed to be able to apply the acquired knowledge in improving the outcome of IVF procedures.

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“…Publications of the Human Reproduction Scientific Research Group by Bódis and Várnagy described the detailed protocol of fertility treatments [67][68][69]. Patient enrolment into IVF procedure was approved by two independent physicians.…”

Section: Fertilization Protocolmentioning

confidence: 99%

Multicausal analysis on psychosocial and lifestyle factors among patients undergoing assisted reproductive therapy – with special regard to self-reported and objective measures of pre-treatment habitual physical activity

et al. 2021

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Background National, regional and global trends in prevalence of infertility indicate its public health importance, however it effects various life dimensions of individuals and couples as well. Lifestyle habits may counteract with these factors. The aim of the study was the multicausal analysis of psychosocial and lifestyle factors undergoing assisted reproductive therapy (ART) with special regard to pre-treatment habitual physical activity (PA). Methods In a cross-sectional, observational cohort study on ART patients (N = 60, age 34.6 ± 5.2 years, BMI 24.2 ± 4.9 kg/m2) with follow up on outcome measures a detailed description was given on PA patterns (ActriGraph GT3X, GPAQ-H) and on general and infertility related distress (BDI-13, FPI). Results Respondents reported normal mood state (BDI-13) but moderately high infertility-related distress (FPI) in Social- and very high distress in Sexual Concern. It was revealed that time spent with recreational PA (RPA) could counteract with infertility-related distress (Social Concern R = -0.378, p = 0.013; Relationship Concern R = -0.365, p = 0.019). In the presence of clinical pregnancy GPAQ-H RPA MET was significantly higher (p = 0.048), in the non-pregnant group cumulative values and work-related PA were higher. Correlations could be found between RPA time and the number of oocytes (R = 0.315, p = 0.045), matured oocytes (R = 0.339, p = 0.030) and embryos (R = 0.294, p = 0.062) by women who reached at least 150 min RPA (GPAQ-H). Multivariate linear regression revealed that the number of oocytes was positively influenced by the GPAQ-H recreation MET (R2 = 0.367; F = 10.994, p = 0.004; B = 0.005, p = 0.004, B Constant = 4.604). Regarding the number of embryos (R2 = 0.757, F = 17.692, p < 0.001, B Constant = 1.342) positive relationship was found with GPAQ-H RPA MET (B = 0.004, p < 0.001) and negative with BMI (B = -0.167, p = 0.038). It was disclosed (R2 = 0.958, F = 408.479, p < 0.001) that higher Very Vigorous Activity (ActiGraph) was accompanied with higher hCG (B = 63.703, p ≤ 0.001). However, time spent with moderate PA (GPAQ-H) (B = 0.002, SE = 0.001, Wald = 3.944, p = 0.047, OR = 1.002) was significantly associated with live births. Conclusions Amount of PA alone did not have a positive effect on outcome of ART. Type and intensity seemed to be more significant. Existing differences in response to infertility due to recreational PA suggest the importance of the development of a specific intervention. The robust overestimation of PA in self-reports highlights the need to improve physical literacy of women undergoing ART.

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Levels of total antioxidant capacity and 8-hydroxy-2′-deoxyguanosine of serum and follicular fluid in women undergoing in vitro fertilization: focusing on endometriosis

Cited by 26 publications

References 34 publications

Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

Oxidative stress and DNA damage status in couples undergoing in vitro fertilization treatment

Antimüllerian Hormone and Oxidative Stress Biomarkers as Predictors of Successful Pregnancy in Polycystic Ovary Syndrome, Endometriosis and Tubal Infertility Factor

Multicausal analysis on psychosocial and lifestyle factors among patients undergoing assisted reproductive therapy – with special regard to self-reported and objective measures of pre-treatment habitual physical activity

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