Levels of Serum Interleukin (IL)‐6 and Gingival Crevicular Fluid of IL‐1β and Prostaglandin E<sub>2</sub> Among Non‐Smoking Subjects With Gingivitis and Type 2 Diabetes

Andriankaja, O. M.; Barros, Silvana P.; Moss, Kevin; Panagakos, Fotinos; DeVizio, William; Beck, James D.; Offenbacher, Steven

doi:10.1902/jop.2009.080385

Cited by 60 publications

(45 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One study [33] showed the possibility that high levels of IL-6 in GCF indicate disease activity. Another study suggested that IL-6 is a key mediator that orchestrates the transition from acute to chronic inflammation and is elevated in periodontitis [34]. In the present study, our data showed that the mean level of IL-8 was significantly higher in diseased sites as compared to healthy sites.…”

Section: Discussionsupporting

confidence: 61%

Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis

Fujita

Sekino

et al. 2011

Odontology

View full text Add to dashboard Cite

The gingival crevicular fluid (GCF) contains various biomarkers, such as interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor-α (TNF-α), and IL-10, among others. These cytokines have been reported to correlate with gingival inflammation and periodontal status. Therefore, the analysis of GCF may be useful for the diagnosis of periodontal status. Pentraxin 3 (PTX3) is the first identified long pentraxin, and is released by several cell types in response to proinflammatory signals. The aim of this study was to determine the levels of IL-1β, IL-6, IL-8, TNF-α, IL-10 and PTX3 in GCF from diseased and healthy sites in patients with chronic periodontitis. Cross-sectional clinical data were obtained from 50 patients with chronic periodontitis. GCF samples were collected with paper strips from one periodontal diseased site and one periodontally healthy site per subject. The levels of IL-1β, IL-6, IL-8, IL-10 and TNF-α were determined using a multiplexed bead immunoassay, and the PTX3 level was measured using an enzyme-linked immunosorbent assay. Mean clinical parameters were significantly higher at diseased sites (P < 0.01) as compared to healthy sites, and the mean levels of PTX3, IL-1β, IL-6, IL-8, IL-10 and TNF-α were higher in diseased sites (P < 0.01) than in healthy sites. There were strong correlations between PTX3 or IL-1β and periodontal status. These results suggest that GCF PTX3 levels might be useful as a diagnostic marker for periodontal disease.

show abstract

Section: Discussionsupporting

confidence: 61%

Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis

Fujita

Sekino

et al. 2011

Odontology

View full text Add to dashboard Cite

show abstract

“…Most reports focus on chronic periodontitis in diabetes, but a limited number include gingivitis (Andriankaja et al 2009) and aggressive periodontitis (Davies et al 2011). The majority are cross-sectional studies and are designed to assess whether diabetes quantitatively or qualitatively influences the cytokine profile of patients with periodontal disease.…”

Section: Cytokines and Adipokinesmentioning

confidence: 99%

A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes

Taylor

Preshaw

Lalla

2013

Journal of Periodontology

211

258

View full text Add to dashboard Cite

A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes Taylor JJ, Preshaw PM, Lalla E. A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes.Abstract Aims: To review the evidence for the molecular and cellular processes that may potentially link periodontal disease and diabetes. The pathogenic roles of cytokines and metabolic molecules (e.g. glucose, lipids) are explored and the role of periodontal bacteria is also addressed. Paradigms for bidirectional relationships between periodontitis and diabetes are discussed and opportunities for elaborating these models are considered. Methods: Database searches were performed using MeSH terms, keywords, and title words. Studies were evaluated and summarized in a narrative review. Results: Periodontal microbiota appears unaltered by diabetes and there is little evidence that it may influence glycaemic control. Small-scale clinical studies and experiments in animal models suggest that IL-1b, TNF-a, IL-6, OPG and RANKL may mediate periodontitis in diabetes. The AGE-RAGE axis is likely an important pathway of tissue destruction and impaired repair in diabetesassociated periodontitis. A role for locally activated pro-inflammatory factors in the periodontium, which subsequently impact on diabetes, remains speculative. Conclusion: There is substantial information on potential mechanistic pathways which support a close association between diabetes and periodontitis, but there is a real need for longitudinal clinical studies using larger patient groups, integrated with studies of animal models and cells/tissues in vitro.

show abstract

“…Our recent findings showed that sIL-6R levels in gingival crevicular fluid (GCF) of inflamed sites are significantly higher than in the healthy sites in periodontitis patients [7]. Importantly, it has been reported that both IL-1β and IL-6 levels in GCF were significantly elevated in diabetic patients [8,9]. Although synergistic effects of these cytokines may induce the severe periodontitis in diabetic patients, the relationship of diabetic conditions such as high glucose (HG) to the cytokine cascades are not fully understood.…”

Section: Introductionmentioning

confidence: 97%

High Glucose-Mediated Cytokine Regulation in Gingival Fibroblasts and THP-1 Macrophage: a Possible Mechanism of Severe Periodontitis with Diabetes

Lew

Naruishi

Kajiura

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Diabetic patients are susceptible to severe periodontitis, but the precise mechanism is not fully understood. Aim of this study was to explore the biological pathogenesis of severe periodontitis in diabetic patients focusing on the crosstalk of human gingival fibroblasts (HGFs) and macrophages. Methods: A total of 70 periodontitis patients with or without diabetes mellitus (DM) were enrolled, and the statistical relationships of diabetic conditions to the periodontal inflammatory parameters were examined by cross-sectional study. In in vitro study, HGFs cell line CRL-2014® (ATCC) and differentiated THP-1 macrophages were cultured with normal glucose (NG: 5.5 mM) or high glucose (HG: 25 mM) condition, and treated with indicated inflammatory factors such as calprotectin (CPT), interleukin (IL)-1β and IL-6. To examine the effects of HG on soluble IL-6 receptor (sIL-6R) production in THP-1 macrophages, the supernatants were collected and the sIL-6R levels were measured by ELISA. To examine the effects of HG on IL-1β or IL-6-induced matrix metalloproteinase (MMPs) production in HGFs, the supernatants were collected. Levels of MMP-1 and tissue inhibitor of MMP-1 (TIMP-1) were measured by ELISA. Finally, after conditioned medium (CM) from THP-1 macrophages cultured with NG or HG conditions was collected, HGFs were treated with the CM. The supernatants were collected 24 hours later and the levels of MMP-1 and TIMP-1 were measured. To examine the specific effects of IL-1β contained in CM on MMP-1 and TIMP-1 production in HGFs, IL-1 receptor antagonist (IL-1ra) was used. Results: There were statistical correlation between IL-1β and sIL-6R levels in gingival crevicular fluid (GCF) and HbA1c in periodontitis patients with DM (IL-1β: P=0.035, sIL-6R: P=0.040). HG and CPT significantly induced sIL-6R production in THP-1 macrophages. HG significantly enhanced IL-1β or IL-6/sIL-6R-induced MMP-1 production in HGFs. The increase of MMP-1 by both IL-1β and IL-6/sIL-6R was significantly inhibited by specific ERK or IκB inhibitors. Corresponding to the regulation of MMP-1 production, HG condition increased the phosphorylation of p44/42 MAPK and IκBα in HGFs treated with IL-1β or IL-6/sIL-6R. Finally, MMP-1 production in HGFs cultured with HG increased significantly by CM from THP-1 macrophages cultured with HG. The induction of MMP-1 by the CM from THP-1 macrophages cultured with HG was significantly inhibited by dose dependent of IL-1ra in HGFs cultured with HG. Conclusion: Diabetic conditions such as HG induce IL-1β and sIL-6R production from macrophages in inflammatory periodontal tissues and may exacerbate the periodontitis synergistically via MMP-1 production from HGFs.

show abstract

Levels of Serum Interleukin (IL)‐6 and Gingival Crevicular Fluid of IL‐1β and Prostaglandin E₂ Among Non‐Smoking Subjects With Gingivitis and Type 2 Diabetes

Cited by 60 publications

References 31 publications

Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis

Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis

A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes

High Glucose-Mediated Cytokine Regulation in Gingival Fibroblasts and THP-1 Macrophage: a Possible Mechanism of Severe Periodontitis with Diabetes

Contact Info

Product

Resources

About

Levels of Serum Interleukin (IL)‐6 and Gingival Crevicular Fluid of IL‐1β and Prostaglandin E2 Among Non‐Smoking Subjects With Gingivitis and Type 2 Diabetes

Cited by 60 publications

References 31 publications

Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis

Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis

A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes

High Glucose-Mediated Cytokine Regulation in Gingival Fibroblasts and THP-1 Macrophage: a Possible Mechanism of Severe Periodontitis with Diabetes

Contact Info

Product

Resources

About

Levels of Serum Interleukin (IL)‐6 and Gingival Crevicular Fluid of IL‐1β and Prostaglandin E₂ Among Non‐Smoking Subjects With Gingivitis and Type 2 Diabetes