Levels of SERPIN family proteins in peri‐implant crevicular fluid in patients with peri‐implantitis

Abstract

Purpose Serine protease inhibitors (SERPINs) family has been discovered in many disorders with proteolysis mechanisms. Our study determined the SERPINBs protein expression via public‐based GEO databases and further validated by peri‐implant crevicular fluid (PICF) of peri‐implantitis patients and healthy recruiters. Methods This study is a retrospective analysis. A total of 123 participants of Fujian Medical University Fujian Stomatological Hospital, consisting of 58 cases of peri‐implantitis and 65 samples of healthy control were retrospectively analyzed by ELISA assays and explored the gene enrichment pathways and clinical significance of SERPINBs expression accompanied by two different cytokines (IL‐6 and TNF‐α). Moreover, the clinical significance of SERPINBs was evaluated in peri‐implantitis patients with PICF samples by the receiver operating curve (ROC) using the area under the curve (AUC). Results KEGG database showed that Starch and sucrose metabolism, Retrograde endocannabinoid signaling, Prion diseases, Pentose phosphate pathways, and Olfactory pathways are up‐regulated; GO database showed that synapse organization, synapse assembly, sequestering of triglyceride, sensory perception of smell, and regulation of synapse organization pathways are up‐regulated. SERPINBs were overexpressed in peri‐implant tissues and peri‐implantitis patients with PICF. SERPINBs was positively correlated to IL‐6 and TNF‐α in peri‐implantitis patients with PICF. The ROC‐AUCs of SERPINBs achieved a significantly higher range from 0.895 to 0.939 in peri‐implantitis patients with PICF. Therefore, certain SERPINBs expressions were not only perceived through PICF and peri‐implant tissues but also showed potential significance in peri‐implantitis. Conclusion SERPINBs play an influential role in the pathogenesis of peri‐implantitis via binding with other inflammatory cytokines.

Effect of Processing Methods of Human Saliva on the Proteomic Profile and Protein-Mediated Biological Processes

“…A superfamily of protease inhibitors (SERPINB) is responsible for many inflammatory processes, and it is also found overexpressed in oral squamous cell carcinoma . A recent report has validated the up-regulated expression of SERPINBs in peri-implant crevicular fluid samples from patients diagnosed with peri-implantitis (inflammatory disease around dental implants in the oral cavity) . The association between overexpressed SERPINBs and increased IL-6 and TNF-α as pro-inflammatory cytokine production could suggest an indirect effect of isolated salivary proteins on deleterious cell function.…”

“…63 A recent report has validated the up-regulated expression of SERPINBs in peri-implant crevicular fluid samples from patients diagnosed with peri-implantitis (inflammatory disease around dental implants in the oral cavity). 64 The association between overexpressed SERPINBs and increased IL-6 and TNF-α as pro-inflammatory cytokine production could suggest an indirect effect of isolated salivary proteins on deleterious cell function. Although the causes and consequences involved in the dysregulation of salivary proteins in soft tissue cells remain unknown, our confocal image has distinctly provided insights regarding the impact of exclusive proteins of pasteurized saliva on cell morphology and viability, which must be further explored in the future.…”

Current Molecular, Cellular and Genetic Aspects of Peri-Implantitis Disease: A Narrative Review

“…In peri-implantitis, however, the concentrations of IL-6 in peri-implant cervical fluid (PICF) are more substantial. Subsequent research confirms this trend, with some studies extending the comparisons to peri-implant mucositis [25,[35][36][37][38][39][40][41][42][43]. Among all the research found, only one study did not find distinctively elevated levels of IL-6.…”

“…Tumor Necrosis Factor α (TNFα) TNFα is the third main inflammatory cytokine one can detect in inflamed tissues. Peri-implant tissues are characterized by overall elevated levels of TNFα, both in soft and hard tissues, compared to healthy implants [30,36,41,43,46,50]. Upon comparing the concentrations of TNFα between periodontitis and peri-implantitis, greater concentrations were noticed in the peri-implantitis group [40].…”

“…The four mentioned SERPINB expressions are also positively correlated with IL-6 and TNFα levels in inflamed tissues, with the highest correlation noted for SERPINB1. In this context, SERPINBs with cytokines may modulate the progression of peri-implant disease [41].…”