Levels of inflammatory cytokines, adrenal steroids, and mRNA for GRα, GRβ and 11βHSD1 in TB pleurisy

D’Attilio, Luciano; Díaz, Ariana; Santucci, Natalia; Bongiovanni, Bettina; Gardeñez, Walter; Marchesini, Marcela M.; Bogué, Cristina; Didoli, Griselda; Bay, María Luisa

doi:10.1016/j.tube.2013.07.008

Cited by 8 publications

(8 citation statements)

References 42 publications

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“…Our results indicates that B. abortus was able to increase GRα/β and 11β-HSD1/2 ratio in osteoblasts suggesting an augmented intracellular concentration of cortisol. This increase in GRα/β and 11β-HSD1/2 expression was also observed in mononuclear cells from patients infected with M. tuberculosis ( 41 ). DHEA treatment reduced GRα/β and 11β-HSD1/2 ratio in infected osteoblast.…”

Section: Discussionsupporting

confidence: 54%

Inhibition of Osteoblast Function by Brucella abortus is Reversed by Dehydroepiandrosterone and Involves ERK1/2 and Estrogen Receptor

et al. 2018

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Brucella abortus induces an inflammatory response that stimulates the endocrine system resulting in the secretion of cortisol and dehydroepiandrosterone (DHEA). Osteoarticular brucellosis is the most common presentation of the active disease in humans, and we have previously demonstrated that B. abortus infection inhibits osteoblast function. We aimed to evaluate the role of cortisol and DHEA on osteoblast during B. abortus infection. B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus infection on osteoblast by increasing their proliferation, inhibiting osteoblast apoptosis, and reversing the inhibitory effect of B. abortus on osteoblast differentiation and function. By contrast, cortisol increased the effect of B. abortus infection. Cortisol regulates target genes by binding to the glucocorticoid receptor (GR). B. abortus infection inhibited GRα expression. Cell responses to cortisol not only depend on GR expression but also on its intracellular bioavailability, that is, dependent on the activity of the isoenzymes 11β-hydroxysteroid dehydrogenase (HSD) type-1, 11β-HSD2 (which convert cortisone to cortisol and vice versa, respectively). Alterations in the expression of these isoenzymes in bone cells are associated with bone loss. B. abortus infection increased 11β-HSD1 expression but had no effect on 11β-HSD2. DHEA reversed the inhibitory effect induced by B. abortus infection on osteoblast matrix deposition in an estrogen receptor- and ERK1/2-dependent manner. We conclude that DHEA intervention improves osteoblast function during B. abortus infection making it a potential candidate to ameliorate the osteoarticular symptoms of brucellosis.

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Section: Discussionsupporting

confidence: 54%

Inhibition of Osteoblast Function by Brucella abortus is Reversed by Dehydroepiandrosterone and Involves ERK1/2 and Estrogen Receptor

et al. 2018

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“…Studies have demonstrated that dexamethasone increases the in vitro activity of 11β-HSD1 and that the application of dexamethasone in early KD could promote the activation of 11β-HSD1 to inhibit acute inflammatory responses 19)20). Therefore, the application of low-dose exogenous GC in early KD might have anti-inflammatory effects 21). The results of this study also show that GRα expression was significantly higher in the CAL group than in the CAN group.…”

Section: Discussionsupporting

confidence: 54%

Changes in 11β-Hydroxysteroid Dehydrogenase and Glucocorticoid Receptor Expression in Kawasaki Disease

Wang

Zhou

et al. 2017

Korean Circ J

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Background and ObjectivesThis study aims to investigate the significance of changes in the expression 11β-hydroxysteroid dehydrogenase (11β-HSD) and glucocorticoid receptor (GR) for the development of Kawasaki disease (KD).Subjects and MethodsReal-time polymerase chain reaction was performed to determine the mRNA expression levels of GR and 11β-HSD in peripheral blood monocytes, both in the acute phase of the disease and after treatment. Western blotting was performed to determine the protein expression levels of GR and 11β-HSD.ResultsThe expression levels of GRβ, GRβ, and 11β-HSD1 mRNA in the acute phase were significantly higher than levels at baseline (p<0.01) and after treatment (p<0.05). The 11β-HSD2 mRNA levels were lower in the acute phase than in the normal group (p<0.01), and they were significantly higher after treatment than before (p<0.01). Western blot results were consistent with the real-time PCR results. The coronary artery lesion group exhibited significantly different 11β-HSD2 expression levels from that of the group with normal coronary arteries (p<0.01).ConclusionGR and 11β-HSD expression changes in the acute phase of KD are important factors for regulating inflammatory responses in KD.

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“…In osteocytes, B. abortus increased GRα/β and 11β-HSD1/2 ratio suggesting an increase in intracellular concentration of cortisol. The increase observed in GRα/β and 11β-HSD1/2 ratio was not exclusive of osteocytes infected with B. abortus, since it was also observed in mononuclear cells from patients with M. tuberculosis infection and also in osteoblast infected with B. abortus[25,31]. Our results demonstrated the importance of the increase of 11β-HSD1/2 ratio induced by B. abortus infection in osteocytes, since in experiments performed with cortisone instead of cortisol; we demonstrated that cortisone could mimic the effect of cortisol on osteocytes during B. abortus infection.…”

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confidence: 85%

Endocrine modulation of Brucella abortus-infected osteocytes function and osteoclastogenesis via modulation of RANKL/OPG

Viglietti

Giambartolomei

Delpino

2019

Microbes and Infection

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Levels of inflammatory cytokines, adrenal steroids, and mRNA for GRα, GRβ and 11βHSD1 in TB pleurisy

Cited by 8 publications

References 42 publications

Inhibition of Osteoblast Function by Brucella abortus is Reversed by Dehydroepiandrosterone and Involves ERK1/2 and Estrogen Receptor

Inhibition of Osteoblast Function by Brucella abortus is Reversed by Dehydroepiandrosterone and Involves ERK1/2 and Estrogen Receptor

Changes in 11β-Hydroxysteroid Dehydrogenase and Glucocorticoid Receptor Expression in Kawasaki Disease

Endocrine modulation of Brucella abortus-infected osteocytes function and osteoclastogenesis via modulation of RANKL/OPG

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