Levels of eosinophil cationic protein and myeloperoxidase from chronic middle ear effusion in patients with allergy and/or acute infection

Abstract

Middle ear eosinophil cationic protein may be used as a marker of related allergy.

Eosinophils and neutrophils in biopsies from the middle ear of atopic children with otitis media with effusion

“…In allergic asthma and in allergic rhinitis eosinophils dominate. The present study shows in OME a covariation between the occurrence of eosinophils and neutrophils (Table 1) and supports the findings in effusions described by Hurst and Venge [12]. A possible explanation for this may be a different role for bacteria or viruses in OME as Fig.…”

“…The use of specific antibodies to granule proteins in eosinophils and neutrophils makes it possible to demonstrate the presence of these cells and their products with greater certainty than is possible with purely morphological techniques, in particular when the cells are degranulating. Hurst and Venge [22] demonstrated, using immunological techniques, the presence of ECP in effusion from OME patients, and in a later study [12] confirmed this finding and also showed the presence of MPO in such effusions. In a recent pilot study, Hurst and Fredens [13] used polyclonal antibodies to demonstrate the presence of ECP positive cells in the lining of the middle ear of OME patients.…”

“…Elevated levels of ECP were found in middle ear effusion of patients with OME [12], but the mechanisms underlying these are not clear. It has been questioned whether the ECP in the effusion arises from the middle ear lining and reflects the presence and activation of eosinophils in the middle ear [13].…”

Pleural-fluid myeloperoxidase in complicated and noncomplicated parapneumonic pleural effusions

“…An increased MPO concentration is an indirect, but clear indication of MPO release by an inflammatory process. MPO, as a marker of inflammation, has been measured to distinguish otitis media, chronic sinusitis, chronic bronchitis and peritonitis of bacterial origin [10][11][12], in which excellent correlations have been observed with levels of IL-8, a cytokine involved not only in chemotaxis, but also in neutrophil activation.…”

“…MPO and reduced nicotinamide-adenine dinucleotide phosphate (NADP-H) oxidase are the main proteins that participate in oxidative-type microbicidal activity [9]. Increased concentrations of MPO have been reported in various inflammatory processes [10][11][12]. The authors previously detected higher levels of pleuralfluid MPO in complicated bacterial effusions than in noninfective effusions [13].…”