Level ofOpisthorchis infestation and carcinogen dose-dependence of cholangiocarcinoma induction in Syrian golden hamsters

Thamavit, Witaya; Kongkanuntn, Rungtiva; Tiwawech, Danai; Moore, Malcolm A

doi:10.1007/bf02899196

Cited by 70 publications

(61 citation statements)

References 25 publications

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“…In several of the hamster studies, although fluke infection per se did not induce liver cancer, the combination of liver fluke infection and exposure to various carcinogenic nitrosamines induced cholangiofibrosis, cholangiocarcinomas, and hepatocellular nodules (9,10,(46)(47)(48). The high prevalence of Helicobacter spp.…”

Section: Discussionmentioning

confidence: 99%

Chronic Hepatitis, Hepatic Dysplasia, Fibrosis, and Biliary Hyperplasia in Hamsters Naturally Infected with a Novel Helicobacter Classified in the H. bilis Cluster

et al. 2009

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We recently described helicobacter-associated progressive, proliferative, and dysplastic typhlocolitis in aging (18-to 24-month-old) Syrian hamsters. Other pathogens associated with typhlocolitis in hamsters, Clostridium difficile, Lawsonia intracellularis, and Giardia spp., were not indentified. The presence of Helicobacter genusspecific DNA was noted by PCR in cecal and paraffin-embedded liver samples from aged hamsters by the use of Helicobacter-specific PCR primers. By 16S rRNA analysis, the Helicobacter sp. isolated from the liver tissue was identical to the cecal isolates from hamsters. The six hamster 16S rRNA sequences form a genotypic cluster most closely related to Helicobacter sp. Flexispira taxon 8, part of the Helicobacter bilis/H. cinaedi group. Livers from aged helicobacter-infected hamsters showed various stages of predominantly portocentric and, to a lesser extent, perivenular fibrosis. Within nodules, there was cellular atypia consistent with nodular dysplasia. The livers also exhibited a range of chronic active portal/interface and lobular inflammation, with significant portal hepatitis being present. The inflammation was composed of a mixture of lymphocytes, neutrophils, and macrophages, indicative of its chronic-active nature in these aged hamsters infected with Helicobacter spp. The isolation of novel Helicobacter spp., their identification by PCR from the diseased livers of aged hamsters, and their taxonomic classification as belonging to the Helicobacter bilis cluster strengthen the argument that H. bilis and closely related Helicobacter spp. play an etiological role in hepatobiliary disease in both animals and humans.We recently described progressive, proliferative, and dysplastic typhlocolitis in aging (18-to 24-month-old) Syrian hamsters (31). The lesions in these hamsters were more severe in the older animals aged 7 to 24 months than in the younger, 1-to 6-month-old hamsters. The presence of Helicobacter spp. in the large bowel of all 24 hamsters included in the study was confirmed by culture and Helicobacter-specific PCR (31). Other pathogens associated with typhlocolitis in hamsters, Clostridium difficile, Lawsonia intracellularis, and Giardia spp., were not indentified in this study (31). Enterohepatic Helicobacter spp. are associated with the development of inflammatory bowel disease in mice and, as determined more recently, humans (2, 18, 27, 51). These helicobacters can also induce hepatitis and hepatocellular carcinoma in susceptible strains of mice (1,14,19). We undertook a study to examine in detail the histological profile of the livers of hamsters aged 18 to 24 months and to determine whether Helicobacter sp. DNA was present in their livers (31). In addition, we purchased a small number of 6-month-old hamsters from the same vendor which originally supplied the aged hamsters and examined them for the presence of Helicobacter spp. in their intestines and liver and whether inflammation was associated with the presence of Helicobacter spp. in these target tissues. We document t...

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Section: Discussionmentioning

confidence: 99%

Chronic Hepatitis, Hepatic Dysplasia, Fibrosis, and Biliary Hyperplasia in Hamsters Naturally Infected with a Novel Helicobacter Classified in the H. bilis Cluster

et al. 2009

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“…9,10 Therefore, a few behavioural modifications other than the cessation of eating Adjusted for anti-OV Ab, smoking and alcohol drinking when calculating the OR of other variables. When each of the 3 variables including anti-OV Ab, smoking and alcohol was examined in terms of interaction with polymorphism of GSTT1, the other 2 variables were adjusted for.-2 CI 5 confidence interval.…”

Section: Discussionmentioning

confidence: 99%

“…10 Diet and cigarette smoking are major sources of nitrosamine exposure, 11 and several foods and foodstuffs consumed in the study area reportedly contain nitrosamines. 12 Ethanol may inhibit first-pass clearance in the liver and allow carcinogens to pass to the peripheral blood.…”

mentioning

confidence: 95%

Genetic and environmental determinants of risk for cholangiocarcinoma via Opisthorchis viverrini in a densely infested area in Nakhon Phanom, northeast Thailand

Honjo

Srivatanakul

Sriplung

et al. 2005

Intl Journal of Cancer

161

133

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Infection with Opisthorchis viverrini (OV) is associated with cholangiocarcinoma. OV is common in northeast Thailand, but less than 10% of the inhabitants develop cholangiocarcinoma. Animal experiments suggest that OV infection alone does not cause cholangiocarcinoma, and thus other environmental and genetic factors may play a role in causation. We conducted a population-based case-control study in which sex, age and place of residence were matched individually. Polymorphisms of GSTM1 and GSTT1 alone were not associated with risk for cholangiocarcinoma, while an elevated level of antibodies against OV (ELISA) 0.200 was the strongest risk indicator (odds ratio as compared to that <0. Key words: population-based case-control study; inflammation; polymorphism; alcohol drinking; nitrosamine Cholangiocarcinoma is uncommon, and the aetiology has been elucidated only for a limited portion of cases.1 Northeast Thailand has an exceptionally high incidence of liver cancer of up to 87.5 (male) and 37.2 (female) per 100,000 (world population-adjusted incidence rate). Most of these cases have been attributed to cholangiocarcinoma.2 Infestation of the liver fluke, Opisthorchis viverrini (OV), has been causally related to cholangiocarcinoma in this region. 3 The prevalence of OV infection in Nakhon Phanom province was between 30 and 70% with substantial variation between villages; 4 the technique for estimation of prevalence was not explicitly mentioned but supposed OV egg counting in the faeces. However, it is unknown why only a limited proportion of the inhabitants (<10%) develop cholangiocarcinoma.In our study, we hypothesised that genetic background may be a contributing factor in the occurrence of the cholangiocarcinoma. Hence, we examined the association between polymorphisms of genes for glutathione-S-transferase enzymes, GSTM1 and GSTT1, and the risk for cholangiocarcinoma. Because both enzymes function in carcinogen detoxification systems, 5 it seems reasonable to speculate that null variants of these genes may be associated with increased risk of cancer. 6The association between OV infection and cancer risk has been a common assumption, but adequate analytic epidemiological studies are sparse. Parkin and colleagues 7 reported in their casecontrol study that past or present infection with OV, as indicated by serum antibodies against OV, is associated with a 5-fold increase in cancer risk. Although the anti-OV antigen titre was elevated and reached a plateau at an approximately 2 months after OV inoculation in hamsters, 8 the transition of the antibody level over the lifespan remains uncertain in both humans and animals. We thus examined the risk of foods possibly containing OV metacercariae as well as that of OV infection as indicated by serum antibody response.In animal experiments, OV infection causes cholangiocarcinoma with coadministration of nitrosamine 9 in a dose-dependent manner.10 Diet and cigarette smoking are major sources of nitrosamine exposure, 11 and several foods and foodstuffs consumed in the study ar...

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“…Some investigations (28,61,110,113) have reported synergism between liver fluke infection and exogenous chemical carcinogens with respect to cholangiocarcinoma development in hamsters. Lee et al (61) demonstrated a high incidence (75%) of cholangiocarcinoma in hamsters that were treated with N-nitrosodimethylamine and then infected with C. sinensis.…”

Section: Experimental Studiesmentioning

confidence: 99%

Clonorchiasis and Cholangiocarcinoma: Etiologic Relationship and Imaging Diagnosis

et al. 2004

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Despite a gradual decrease in prevalence, clonorchiasis is still prevalent in East Asia. A large and compelling body of evidence links clonorchiasis and cholangiocarcinoma, although the mechanisms involved are not completely understood. Clonorchiasis induces biliary epithelial hyperplasia and metaplasia, and this could facilitate at least one stage of the carcinogenesis, which is promoting effect. In areas of endemic infection, more clonorchiasis cases are now diagnosed incidentally during radiological examinations such as cholangiography, ultrasonography, and computed tomography. Radiological findings are regarded as pathognomonic for clonorchiasis since they reflect the unique pathological changes of this disorder. These radiological examinations currently play important roles in the diagnosis, staging, and decision-making process involved in the treatment of cholangiocarcinoma. The morphological features and radiological findings of clonorchiasis-associated cholangiocarcinoma are essentially combinations of the findings for the two diseases. The morphological features of clonorchiasis- associated cholangiocarcinoma, observed in radiological examinations, do not differ from those of the usual cholangiocarcinoma. In patients diagnosed with or suspected to have clonorchiasis, radiological findings should be carefully scrutinized for occult cholangiocarcinoma

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Level ofOpisthorchis infestation and carcinogen dose-dependence of cholangiocarcinoma induction in Syrian golden hamsters

Cited by 70 publications

References 25 publications

Chronic Hepatitis, Hepatic Dysplasia, Fibrosis, and Biliary Hyperplasia in Hamsters Naturally Infected with a Novel Helicobacter Classified in the H. bilis Cluster

Chronic Hepatitis, Hepatic Dysplasia, Fibrosis, and Biliary Hyperplasia in Hamsters Naturally Infected with a Novel Helicobacter Classified in the H. bilis Cluster

Genetic and environmental determinants of risk for cholangiocarcinoma via Opisthorchis viverrini in a densely infested area in Nakhon Phanom, northeast Thailand

Clonorchiasis and Cholangiocarcinoma: Etiologic Relationship and Imaging Diagnosis

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