Level of overall hemostasis potential in donor and patient plasma in pathology

Abstract

Coagulation potential (CP), overall hemostasis potential (OHP) and fibrinolysis potential (FP) in plasma of donors and patients with myocardial infarction (MI), stroke (St) and hip joint diseases (HJD) have been investigated using M. Blomback’s global hemostasis assay. Plasma samples of the patients were analyzed with APTT reagent in the presence or absence of t-PA. It was found that the ratio of values of СP, OHP and FP in plasma of patients to those of donors plasma were 78, 60 and 123% at MI; 157, 155 and 162% at St; 128, 131 and 124% at HJD. CP to FP ratio that indicated balance between coagulation and fibrinolytic systems activities were 4.13, 2.5, 4.0 and 4.26 in plasma of donors and MI, St and HJD patients, respectively. These results are evidence of increased fibrinolytic activity in plasma of MI patients. Lag-periods of plasma clotting of MI, St and HJD patients were prolonged by 2.3, 7.2 and 1.5-fold, respectively. Pearson’s correlation analysis between parameters, obtained in vitro studies using global hemostasis assay, and concentrations of the molecular markers (soluble fibrin and D-dimer), which formed in vivo in plasma of MI, St and HJD patients, did not reveal any relationship between them.

Overall hemostasis potential of the blood plasma and its relation to some molecular markers of the hemostasis system in patients with chronic renal disease of stage VD

“…In the previous article, we found that between the parameters of the hemostasis potential determined by the method of M. Blomback [4], which show the ability of blood plasma hemostasis system in vitro to form and dissolve the fibrin clot, and molecular markers of the hemostasis system in plasma of blood in vivo, which characterize the state of the hemostasis system at the moment of the blood sample withdrawing in patients with myocardial infarction, stroke or hip joint disease, no strong correlation was observed [4].…”

“…The overall hemostasis potential (OHP) is characterized by the size of area under curve of the clot turbidity from the moment the plasma coagulation is initiated to the moment when turbidity completely disappeared in the presen ce of t-PA. Coagulation potential (CP) is the area under curve of the clot formation in the absence of t-PA from the moment of plasma coagulation initiation to the moment of complete dissolution of the clot in the presence of t-PA. Fibrinolysis potential (FP) is the difference between the magnitudes of CP and OHP. All values of potentials are expressed in units of optical density multiplied by time in seconds (o. u.•s) [4,6].…”

“…There were no correlations found in pairs OHP-sf and OHP-DD. Strong, very strong and sometimes intermediate relationship was observed for pairs within the group of parameters associated with the determination of OHP, namely OHP -H, OHP -L, OHP -T, OHP -CP, OHP -FP, CP -FP, FP -H and FP -L (Table 3), as well as with determination of OHP for patients with myocardial infarction, stroke and hip disease [4]. At the same time, coefficients in groups with different concentrations of soluble fibrin showed the same order of relationship strength as in the group "all patients".…”

Overall hemostasis potential of blood plasma and its connection to molecular markers of the hemostasis system in patients with stenosis of coronary artery

“…Overall hemostasis potential (OHP) was characterized by the area under the turbidity curve of the clot from the moment of initiation of plasma coagulation to the moment of complete destruction of the clot in the presence of t-PA. Coagulation potential (CP) -the area under the curve of clot formation from the moment of initiation of plasma coagulation to the moment of complete dissolution of the clot in the absence of t-PA. Fibrinolytic potential (FP)isthedifferencebetweenthevaluesofCPand OHP. All values were expressed in units of optical density multiplied by time in seconds (o.u.•s) [12,13].…”

“…The individual stages of the process of clot formation and hydrolysis were characterized by thefollowingparameters:τ(s)-lagperiod,which shows the rate of activation of the internal path of the coagu lationcascade,thrombinformation,fibrinogenactivationintofibrinandprotofibrilformation; α(V 1 ) o.u./s -tg of the angle of inclination of the maximum rate of growth of the clot turbidity, which showstherateoflateralassociationofprotofibrils andtheformationoffibrils;H(o.u. )-themagnitude ofthefinalturbidityoftheclot,whichisdetermined bythethicknessofthefibrilsoftheclot;L(s)isthe half-life of the clot, which depends on the concentration of plasminogen, t-PA and the structure of theclot;β(-V 2 )-о.u./s-tgoftheangleofinclination of the maximum rate of falling of turbidity of a clot which depends on concentration of plasmin and plasmininhibitors(α2-antiplasmin,PAI-1)inblood plasma [13].…”