Level of Adiponectin, Leptin and Selected Matrix Metalloproteinases in Female Overweight Patients with Primary Gonarthrosis

Abstract

The aim of this study was to assess levels of adiponectin (ACRP-30) and leptin in serum and synovial fluid (SF) of overweight females with primary gonarthrosis (GOA) and to look for their correlations with clinical status and the level of biochemical OA biomarkers: matrix metalloproteinase (MMP) 1, MMP-9 and tissue inhibitor of metalloproteinase (TIMP-1). The studied group consisted of 39 overweight females undergoing primary total knee arthroplasty as a result of GOA. The stage of GOA was evaluated according to Knee Society Score (KSS), Ahlbäck and Kellgren–Lawrence (K-L) scores. Peripheral blood and SF were obtained. Levels of selected biomarkers were assessed using ELISA kits. The mean level of ACRP-30 in serum valued were 8393.80 ng/mL and in SF, 774.33 ng/mL, the mean concentration of leptin in serum was 32,040.74 pg/mL and in SF, 27,332.12 pg/mL. Levels of leptin in serum and SF correlated with body mass index (BMI), (p = 0.0005, and p = 0.0002, respectively). Levels of ACRP-30 in serum was correlated with clinical scores (Ahlbäck: p = 0.0214; K-L: p = 0.0146). ACRP-30 in SF correlated with ACRP-30 in serum (p = 0.0003), tended to negatively correlate with MMP-1 in serum (p = 0.0598) and positively correlate with pro-MMP-1 in SF (p = 0.0600). To conclude, this study confirms the correlations between concentrations of both, leptin and ACRP-30, comparing serum and SF. In overweight females, leptin levels increase with BMI and ACRP-30 serum level increase in more advanced GOA stages. Finally, leptin levels were correlated with TIMP-1 serum concentration, one of the biochemical markers of GOA.

Gonartrozlu Hastalarda Trigliserit Glikoz İndeksi ile Nötrofil Lenfosit Oranı Arasındaki İlişki

“…5,6,8,9 e present study investigated the relationship between e ndings of this study are consistent with the previous studies. [7][8][9][10][11] In the third round of the National Health and Nutrition Examination Survey (NHANES) study , metabolic syndrome was more prevalent in people with gonarthrosis. 11 In the Michigan Bone Metabolism and…”

Elevated fatty acid β-oxidation by leptin contributes to the proinflammatory characteristics of fibroblast-like synoviocytes from RA patients via LKB1-AMPK pathway

“…It is indispensable to explore the key factor leading to enhanced fatty acid oxidation in RA milieu. Leptin has been considered a link between the neuroendocrine and immune systems [32,33], which exhibits proinflammatory and pro-catabolic actions on cartilage [34][35][36]. However, Wang et al showed that leptin-JAK/STAT3 promoted breast cancer stemness and chemoresistance via upregulating lipid metabolism through FAO [19], providing new light to better understanding of the role of lipid metabolic reprogramming.…”

Assessment of the Impact of Physical Activity on the Musculoskeletal System in Early Degenerative Knee Joint Lesions in an Animal Model

“…At the early OA stages, the Hoffa fat pad (IPFP—infrapatellar fat pad) increases its activity. The exact mechanism of the development of these changes in early OA is not clear but may consist in increased production of cytokines, adipokines, interleukins, and growth factors secreted by IPFP, which accelerate cartilage destruction through enhanced production of matrix metalloproteinases [ 1 , 61 , 62 ]. There are also no data showing changes in the IPFP volume in early OA.…”

“…Osteoarthritis (OA) is one of the most prevalent inflammatory diseases of the osteoarticular system and one of the leading causes of disability worldwide, affecting approximately 250 million people [ 1 , 2 , 3 ]. It causes joint pain, limits physical and social activity, and often leads to chronic disability [ 2 ].…”