Levantamento de seio maxilar com enxerto ósseo em neoformação associado a osso bovino inorgânico: avaliação clínica, histológica e histomorfométrica

Abstract

Sinus lift with newly forming bone and inorganic bovine bone: a clinical, histologic and histomorphometric evaluation The aim of this study is to evaluate the efficacy of newly forming bone graft (NFB) in the gain of bone height in sinus lift procedures. It were recruited for this study individuals 25-60 years of age, both genders, presenting a missing tooth at an upper premolar or molar region with 2-9 mm of remaining bone between alveolar ridge crest and sinus floor and the existence of an edentulous ridge or at least one tooth condemned to extraction. Sinus were treated by NFB mixed to inorganic bovine bone-IBB (test; n= 7) or IBB (control; n= 6). The volume of bone tissue was evaluated by computerized tomography obtained at baseline examination and 6 months after surgery. After this period, biopsies of hard tissue were obtained during implant installation for histologic and histomorphometric analysis. The analysis of data from tomographic images by t test showed that both materials were equally effective in the gain of bone height (test: 11.22 ± 0.60 mm vs. control: 11.82 ± 0.69 mm; p= 0.0664). The histologic analysis of tissue reaction around and between graft particles showed absence of significant differences between groups, according to Mann Whitney. Histomorphometric analysis showed greater percentage of vital bone (31.42% ± 11.13% vs. 16.38% ± 10.14%; p= 0.0002) and lower percentage of remaining particles (1.32% ± 2.34% vs. 3.15% ± 3.31%; p= 0.0306) and connective tissue (27.65% ± 12.34% vs. 35.02 ± 13.16; p= 0.0257) at test than control group. The mean diameter of remaining particles was greater at control than test group, according to t test (p= 0.0294), although no differences were observed between groups related to the area of direct contact between new bone and particles surface. These findings suggest that NFB is effective in the gain of bone height in sinus lift procedures, resulting in the formation of greater amount of vital bone with the use of IBB alone.

Efeitos do PDGF-BB na taxa de proliferação e na adesão de células derivadas da granulação óssea a fragmentos radiculares

“…Conforme previamente evidenciado em estudos histológico (Passanezi et al 1989) e clínicos (Passanezi et al 1989, Ferraz 2009Sant'Ana et al 2012;Ferraz 2013), a técnica do enxerto ósseo em neoformação (granulação óssea) resulta em regeneração dos tecidos periodontais, com formação de novo osso, cemento e ligamento (conforme evidenciado em cães), fechamento Dentro da metodologia empregada neste estudo, pode-se concluir que:…”