Levan Nanostructured Thin Films by MAPLE Assembling

Sima, Félix; Mutlu, Esra Cansever; Eroğlu, Mehmet S.; Sima, Livia Elena; Serban, N.; Ristoscu, Carmen; Petrescu, Ştefana M.; Öner, Ebru Toksoy

doi:10.1021/bm200340b

Cited by 76 publications

(34 citation statements)

References 26 publications

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“…The OL coatings induced an increased cell activity revealed by enhanced cell proliferation as compared with the simple L coatings. This is in accordance with the higher hydrophilicity of OL surfaces due to the acidic aldehyde-hydrogen bonds forming after oxidation [12].…”

Section: Nanostructured Levan Thin Filmssupporting

confidence: 81%

“…In order to produce functional aldehyde groups, the oxidation of Levan was carried out before laser transfer in dark oven at 50 °C for six days. MAPLE proved to be the only technique able to transfer nanostructured Levan thin coatings on solid substrates, which exhibited biocompatible properties in vitro [12]. The nanostructured aspect of the film that increases the specific surface area can consequently boost the material properties.…”

Section: Nanostructured Levan Thin Filmsmentioning

confidence: 99%

“…Some other application fields are in textiles, cosmetics, wastewater treatment, food, and pharmaceutical industries [46,50,52]. Sima et al [12] reported for the first time on pure and oxidized Levan nanostructured thin films assembling by MAPLE (Section 4.2). The authors evidenced a high potential for cell proliferation for both coatings (with certain predominance for oxidized Levan) by in vitro colorimetric assays.…”

Section: Introductionmentioning

confidence: 99%

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Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Axente¹,

Sima²,

Ristoscu³

et al. 2016

Recent Advances in Biopolymers

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This chapter provides an overview of recent advances in the field of laser-based synthesis of biopolymer thin films for biomedical applications. The introduction addresses the importance of biopolymer thin films with respect to several applications like tissue engineering, cell instructive environments, and drug delivery systems. The next section is devoted to applications of the fabrication of organic and hybrid organic-inorganic coatings. Matrix-assisted pulsed laser evaporation (MAPLE) and Combinatorial-MAPLE are introduced and compared with other conventional methods of thin films assembling on solid substrates. Advantages and limitations of the methods are pointed out by focusing on the delicate transfer of bio-macromolecules, preservation of properties and on the prospect of combinatorial libraries' synthesis in a single-step process. The following section provides a brief description of fundamental processes involved in the molecular transfer of delicate materials by MAPLE. Then, the chapter focuses on the laser synthesis of two polysaccharide thin films, namely Dextran doped with iron oxide nanoparticles and Levan, followed by an overview on the MAPLE synthesis of other biopolymers. The chapter ends with summary and perspectives of this fast-expanding research field, and a rich bibliographic database.

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Section: Nanostructured Levan Thin Filmssupporting

confidence: 81%

Section: Nanostructured Levan Thin Filmsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Axente¹,

Sima²,

Ristoscu³

et al. 2016

Recent Advances in Biopolymers

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“…Levan as a biopolymer can be use as pharmaceutical excipient, drugdelivery carrier and medical implant coating. Sima et al deposited the levan coating by MAPLE process [40]. It displayed good compatibility with bone cells.…”

Section: Recent Progress Of Maple Biomaterialsmentioning

confidence: 99%

Matrix-Assisted Pulsed Laser Evaporation (MAPLE) technique for deposition of hybrid nanostructures

Yang¹,

Zhang²

2017

Front Nanosci Nanotech

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This review paper provides the detailed information on a new nano-manufacturing process for producing nanocomposites, i.e., the matrix-assisted pulsed laser evaporation (MAPLE). Owing to its unique advantages in depositing polymers and biomaterials, MAPLE technique has been applied to fabricate coatings for medical implants, and electronic devices. In this paper, the current progress on the applications of MAPLE technique is reviewed and discussed. MAPLE techniques have demonstrated a precise control of the parameter such as film thickness and roughness, material structure after deposition. The potentials of MAPLE technique in nanobiotechnology are also discussed in this review paper.

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“…Further research on the potential uses of levan produced by Halomonas sp. as a bioflocculating agent (7), its nanostructured thin films (9), its suitability for peptide-based drug nanocarrier systems (8), and a scheme for economical production from waste (4) have been reported. Moreover, the first genome scale metabolic model of Chromohalobacter salexigens DSM 3043 was reported, which in turn formed the basis for the reconstruction of a generic in silico metabolic model of levan-producing Halomonas sp.…”

Section: H Alomonas Smyrnensis Aad6mentioning

confidence: 99%

Draft Genome Sequence of Halomonas smyrnensis AAD6 ^T

et al. 2012

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Turkey b Halomonas smyrnensis AAD6T is a Gram-negative, aerobic, exopolysaccharide-producing, and moderately halophilic bacterium that produces levan, a fructose homopolymer with many potential uses in various industries. We report the draft genome sequence of H. smyrnensis AAD6 T , which will accelerate research on the rational design and optimization of microbial levan production. H alomonas smyrnensis AAD6T was isolated from soil samples taken from the Çamaltı Saltern area (Turkey) and was found to excrete high levels of levan exopolysaccharide (5). Further research on the potential uses of levan produced by Halomonas sp. as a bioflocculating agent (7), its nanostructured thin films (9), its suitability for peptide-based drug nanocarrier systems (8), and a scheme for economical production from waste (4) have been reported. Moreover, the first genome scale metabolic model of Chromohalobacter salexigens DSM 3043 was reported, which in turn formed the basis for the reconstruction of a generic in silico metabolic model of levan-producing Halomonas sp. (1). Recently, H. smyrnensis strain AAD6T was identified as a Gram-negative, nonmotile, dark-yellow-pigmented, aerobic, moderately halophilic bacterium (6).In this study, whole-genome sequencing of H. smyrnensis AAD6 T was performed by using a combination of 454 GS FLXϩ (454 Life Sciences, Branford, CT) and Ion Proton (Ion Torrent Systems, Inc., Guilford, CT) sequencers. The shotgun sequence data generated consisted of 1,082,564 reads (mean length of 498.36 bp) and 359,877 reads (mean length of 122.42 bp), respectively, providing 140-fold coverage. The duplicates in the sequence data were eliminated with CLC Genomics Workbench (v5.0.1). De novo assembly of the whole sequencing reads was carried out with Newbler Assembler (v2.3). One hundred ninetytwo contigs, with a minimum length of 500 bp, were obtained. Alignment of these contigs and elimination of the overlaps using Sequencher 5.0 (3) resulted in 81 high-quality contigs. These contigs were mapped to the raw data with CLC Genomics Workbench (v5.0.1) to estimate the terminals, and using overlapping terminal regions, the contigs were assembled into 34 supercontigs via Geneious Pro (v5.5.6) software. The draft genome of H. smyrnensis AAD6T was structured with 34 supercontigs (total assembly size of 3,561,919 bp). Gene prediction and annotation were carried out using the RAST server (2).The draft genome that resulted had a mean GC content of 67.9%, 3,237 coding sequences, 1,756 subsystems, and 64 RNA genes. Putative functions could be assigned to 2,436 protein-coding genes, whereas 801 hypothetical proteins had no match to any known proteins. Genome analysis revealed a number of genes related to exopolysaccharide biosynthesis, including the genes for levansucrase and ExoD.Phylogenetic analysis of the draft genome revealed a rather close relationship to other species of the genus Halomonas, where Halomonas salina (99.5%) and Halomonas halophila (99.5%) were found to be the closest species on the basis of 16S rRNA sequ...

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Levan Nanostructured Thin Films by MAPLE Assembling

Cited by 76 publications

References 26 publications

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Matrix-Assisted Pulsed Laser Evaporation (MAPLE) technique for deposition of hybrid nanostructures

Draft Genome Sequence of Halomonas smyrnensis AAD6 ^T

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Levan Nanostructured Thin Films by MAPLE Assembling

Cited by 76 publications

References 26 publications

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Biopolymer Thin Films Synthesized by Advanced Pulsed Laser Techniques

Matrix-Assisted Pulsed Laser Evaporation (MAPLE) technique for deposition of hybrid nanostructures

Draft Genome Sequence of Halomonas smyrnensis AAD6 T

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Draft Genome Sequence of Halomonas smyrnensis AAD6 ^T