Leupaxin Expression Is Dispensable for B Cell Immune Responses

Bonaud, Amélie; Clare, Simon; Bisio, Valeria; Sowerby, John M.; Yao, Shugang; Ostergaard, Hanne L.; Balabanian, Karl; Smith, Kenneth G. C.; Espéli, Marion

doi:10.3389/fimmu.2020.00466

Cited by 6 publications

(4 citation statements)

References 36 publications

(37 reference statements)

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“…ELISA assays were performed as previously described 43 for the determination of the different isotype of Ig or NP-specific antibody titres in sera or culture supernatants. Briefly, plates were pre-coated with goat anti-mouse IgM (Southern Biotech) or with NP(15)-BSA (Biosearch Technologies).…”

Section: Elisamentioning

confidence: 99%

Plasma cell maintenance and antibody secretion are under the control of Sec22b-mediated regulation of organelle dynamics

Bonaud

Gargowitsch

Gilbert

et al. 2022

Preprint

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Despite the essential role of plasma cells in health and disease, the cellular mechanisms controlling their survival and secretory capacity are still poorly understood. Here, we identified the SNARE Sec22b as a unique and critical regulator of plasma cell maintenance and function. In absence of Sec22b, plasma cells were barely detectable and serum antibody titres were dramatically reduced. Accordingly, Sec22b deficient mice fail to mount a protective immune response. At the mechanistic level, we demonstrated that Sec22b is indispensable for efficient antibody secretion but also for plasma cell fitness through the regulation of the morphology of the endoplasmic reticulum and mitochondria. Altogether, our results unveil a critical role for Sec22b-mediated regulation of plasma cell biology through the control of organelle dynamics.

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Section: Elisamentioning

confidence: 99%

Plasma cell maintenance and antibody secretion are under the control of Sec22b-mediated regulation of organelle dynamics

Bonaud

Gargowitsch

Gilbert

et al. 2022

Preprint

Self Cite

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show abstract

“…The CD6 gene encodes the T-cell differentiation antigen and regulates the adaptive immune system by promoting activation and proliferation of T cells [ 38 , 39 ]. The LPXN gene negatively regulates the B-cell antigen receptor [ 40 ] and plays an important role in the B-cell immune response [ 41 ]. Furthermore, PTGDR2 encodes the receptor for prostaglandin (PG) D 2 and exerts pro- and anti-inflammatory properties [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

A genome-wide screen for resilient responses in growing pigs

et al. 2022

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Background There is a growing interest to decipher the genetic background of resilience and its possible improvement through selective breeding. The objective of the present study was to provide new insights into the genetic make-up of resilience in growing pigs by identifying genomic regions and candidate genes associated with resilience indicators. Commercial Duroc pigs were challenged with an attenuated Aujeszky vaccine at 12 weeks of age. Two resilience indicators were used: deviation from the expected body weight at 16 weeks of age given the growth curve of non-vaccinated pigs (∆BW) and the increase in acute-phase protein haptoglobin at four days post-vaccination (∆HP). Genome-wide association analyses were carried out on 445 pigs, using genotypes at 41,165 single nucleotide polymorphisms (SNPs) and single-marker and Bayesian multiple-marker regression approaches. Results Genomic regions on pig chromosomes 2, 8, 9, 11 (∆BW) and 8, 9, 13 (∆HP) were found to be associated with the resilience indicators and explained high proportions of their genetic variance. The genomic regions that were associated explained 27 and 5% of the genetic variance of ∆BW and ∆HP, respectively. These genomic regions harbour promising candidate genes that are involved in pathways related to immune response, response to stress, or signal transduction (CD6, PTGDR2, IKZF1, RNASEL and MYD88), and growth (GRB10 and LCORL). Conclusions Our study identified novel genomic regions that are associated with two resilience indicators (∆BW and ∆HP) in pigs. These associated genomic regions harbour potential candidate genes involved in immune response and growth pathways, which emphasise the strong relationship between resilience and immune response.

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“…ELISA assays were performed as previously described ( 47 ) for the determination of the different isotype of Ig or NP-specific antibody titers in sera or culture supernatants. Briefly, plates were precoated with goat anti-mouse IgM (Southern Biotech) or with NP(15)-BSA (Biosearch Technologies).…”

Section: Methodsmentioning

confidence: 99%

Sec22b is a critical and nonredundant regulator of plasma cell maintenance

Bonaud

Gargowitsch

Gilbert

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

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Despite the essential role of plasma cells in health and disease, the cellular mechanisms controlling their survival and secretory capacity are still poorly understood. Here, we identified the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) Sec22b as a unique and critical regulator of plasma cell maintenance and function. In the absence of Sec22b, plasma cells were hardly detectable and serum antibody titers were dramatically reduced. Accordingly, Sec22b -deficient mice fail to mount a protective immune response. At the mechanistic level, we demonstrated that Sec22b contributes to efficient antibody secretion and is a central regulator of plasma cell maintenance through the regulation of their transcriptional identity and of the morphology of the endoplasmic reticulum and mitochondria. Altogether, our results unveil an essential and nonredundant role for Sec22b as a regulator of plasma cell fitness and of the humoral immune response.

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Leupaxin Expression Is Dispensable for B Cell Immune Responses

Cited by 6 publications

References 36 publications

Plasma cell maintenance and antibody secretion are under the control of Sec22b-mediated regulation of organelle dynamics

Plasma cell maintenance and antibody secretion are under the control of Sec22b-mediated regulation of organelle dynamics

A genome-wide screen for resilient responses in growing pigs

Sec22b is a critical and nonredundant regulator of plasma cell maintenance

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