Leukotrienes, allergy and inflammation

Nijkamp, Frans P.; Sitsen, J. M. A.

doi:10.1007/bf01959134

Cited by 7 publications

(3 citation statements)

References 39 publications

(3 reference statements)

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“…Thus, leukotriene concentration increases at inflammation sites (Pirault & Bäck, 2018). LTC 4 , LTD 4 , and LTE 4 are high-reactivity substances present during anaphylaxis (Nijkamp & Sitsen, 1982). They play an essential role in the occurrence of allergy-related inflammations.…”

Section: Lox Inhibition Testmentioning

confidence: 99%

Guidelines for the in vitro determination of anti‐inflammatory activity

Huang,

Li,

Sabier

et al. 2024

eFood

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Chronic inflammation contributes to various chronic diseases in humans. Currently used anti‐inflammatory drugs have several limitations and are not suitable for long‐term use. Anti‐inflammatory foods or extracts can prevent inflammation‐related diseases, offering a promising approach for the management of these pathologies. In vitro anti‐inflammatory experiments have the advantages of short experimental period and low cost for screening anti‐inflammatory foods or drugs. They measure pro‐inflammatory enzyme activity such as lipoxygenase (LOX) and cyclooxygenase (COX), and the production of pro‐inflammatory cytokines such as tumor necrosis factor (TNF) and interleukins (IL). This article reviews in vitro assays used to evaluate the anti‐inflammatory effect, examining the effects of different inflammatory factors and the employed methods, and analyzing their principles, advantages, and disadvantages. Thus, it aims to provide guidelines for screening natural foods with anti‐inflammatory potential.

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Section: Lox Inhibition Testmentioning

confidence: 99%

Guidelines for the in vitro determination of anti‐inflammatory activity

Huang,

Li,

Sabier

et al. 2024

eFood

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“…Аллергические болезни и респираторные инфекции имеют ряд общих и (или) взаимопотенцирующих звеньев патогенеза. Гистамин, лейкотриены, проcтагландины, многие цитокины и хемокины являются медиаторами как аллергического, так и инфекционного воспаления [1][2][3][4][5].…”

Section: Introductionunclassified

Interferons alpha and gamma, pidotimod, and tilorone in the treatment of acute respiratory infections in patients with allergic rhinitis: a prospective, cohort clinical and immunological study

et al. 2022

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Aim. To compare the clinical efficacy and influence on interferon (IFN) production / sensing of drugs with immunemediated antiviral effects, which potentiate type 1 (T1) immune responses, in the treatment of acute respiratory infections (ARI) in patients with allergic rhinitis.Materials and methods. 146 ARI patients with remission of seasonal allergic rhinitis were divided into 4 cohorts. In addition to symptomatic therapy, patients received either 2,000 IU of IFNγ in each nasal passage 5 times a day; or rectal suppositories containing 106 IU of IFN-α2b and antioxidants (AO) twice a day, and a gel with IFN-α2b and AO intranasally 3 times a day; or 400 mg of pidotimod per os twice a day; or 125 mg of tilorone per os on days 1, 2, 4, and 6. The severity of ARI was determined daily as the sum of 10-point scores for 15 symptoms. Serum concentrations of IFNα and IFNγ and the ability of blood cells to produce these cytokines ex vivo spontaneously and upon stimulation with Newcastle disease virus or phytohemagglutinin were studied using enzyme-linked immunosorbent assay (ELISA). The proportions of circulating lymphocytes expressing type I IFN receptor subunit 2 (CD118) or IFNγ receptor α-chain (CD119) were determined by flow cytometry.Results. ARI symptoms in all cohorts generally regressed in a similar way. However, from day 5 of the treatment, pidotimod relieved symptoms more effectively than other drugs. In patients treated with tilorone, the regression of ARI manifestations was delayed in the first two to three days, followed by rapid symptom reduction. An initial decrease in the induced production of IFNγ was found in patients treated with pidotimod, and a tendency to a decrease in this parameter was noted in other cohorts. The induced production of IFNγ after the treatment in all groups did not differ from that in healthy donors. No significant changes and differences in the proportions of CD118+ and CD119+ lymphocytes were found between the cohorts, except for a decrease in the number of CD118+ cells after the treatment with tilorone. In patients treated with IFN-α2b + AO, the proportions of CD119+ and CD118+ lymphocytes tended to increase slightly.Conclusion. Drugs that promote the development of T1 over T2 immune responses are a useful option for treating ARI in patients with allergic rhinitis.

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“…4 arachidonic acid metabolites with 20 carbon chain backbones. 6,7 Platelet-activating factor (PAF), an important lipid mediator not derived from arachidonic acid, plays a similar role in allergic/ inammatory response. 8 Moreover, PAF is both released from and an activator of immune system cells and has the ability to aggregate platelets.…”

Section: Introductionmentioning

confidence: 99%

Isotope-dilution UPLC-MS/MS determination of cell-secreted bioactive lipids

Meyer

Thompson

Wang

et al. 2013

Analyst

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Secreted bioactive lipids play critical roles in cell-to-cell communication and have been implicated in inflammatory immune responses such as anaphylaxis, vasodilation, and bronchoconstriction. Analysis of secreted bioactive lipids can be challenging due to their relatively short lifetimes and structural diversity. Herein, a method has been developed using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to quantify five cell-secreted, structurally and functionally diverse bioactive lipids (PGD2, LTC4, LTD4, LTE4, PAF) that play roles in inflammation. Sample analysis time is 5 min, and isotopically labeled internal standards are used for quantification. This method was applied to an immortal secretory cell line (RBL-2H3), a heterogeneous primary cell culture containing peritoneal mast cells, and murine platelets. In RBL cell supernatant samples, intrasample precisions ranged from 7.32-21.6%, averaging 17.0%, and spike recoveries in cell supernatant matrices ranged from 88.0-107%, averaging 97.0%. Calibration curves were linear from 10 ng mL(-1) to 250 ng mL(-1), and limits of detection ranged from 0.0348 ng mL(-1) to 0.803 ng mL(-1). This method was applied to the determination of lipid secretion from mast cells and platelets, demonstrating broad applicability for lipid measurement in primary culture biological systems.

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Leukotrienes, allergy and inflammation

Cited by 7 publications

References 39 publications

Guidelines for the in vitro determination of anti‐inflammatory activity

Guidelines for the in vitro determination of anti‐inflammatory activity

Interferons alpha and gamma, pidotimod, and tilorone in the treatment of acute respiratory infections in patients with allergic rhinitis: a prospective, cohort clinical and immunological study

Isotope-dilution UPLC-MS/MS determination of cell-secreted bioactive lipids

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