Leukotriene B4 pathway regulates the fate of the hematopoietic stem cells

Chung, Jin Woong; Kim, Geun Young; Mun, Yeung Chul; Ahn, Ji Young; Seong, Chu-Myong; Kim, Jae Hong

doi:10.1038/emm.2005.6

Cited by 37 publications

(20 citation statements)

References 13 publications

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“…Activated metabolites of FFAs play vital roles in many cellular processes such as cell proliferation. Biologically active metabolites of FFAs act as second messengers to promote progenitor cell proliferation and to induce proliferation of human cord blood hematopoietic stem cells [22]. On the other hand, FFAs could decrease the proliferation of neuroblastoma cell [23].…”

Section: Discussionmentioning

confidence: 99%

Linoleic Acid Induces Mouse Embryonic Stem Cell Proliferation Via Ca<sup>2+</sup>/PKC, PI3K/Akt, and MAPKs

Kim

et al. 2009

Cell Physiol Biochem

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Section: Discussionmentioning

confidence: 99%

Linoleic Acid Induces Mouse Embryonic Stem Cell Proliferation Via Ca<sup>2+</sup>/PKC, PI3K/Akt, and MAPKs

Kim

et al. 2009

Cell Physiol Biochem

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“…The resultant supernatants were assayed for protein and β-galactosidase activity. Luciferase activity was assayed in 10-μl samples of extract; the luciferase luminescence was counted in luminometer (Turner Design, TD-20/20) and normalized to the co-transfected β-galactosidase activity, as described elsewhere (Woo et al, 2000b;Chung et al, 2005;Shin et al, 2007). Transfection experiments were performed in triplicate with two independently isolated sets of cells, and the results were averaged.…”

Section: Transient Transfection and Nf-κb Luciferase Activity Assaymentioning

confidence: 99%

TNF-α-induced up-regulation of intercellular adhesion molecule-1 is regulated by a Rac-ROS-dependent cascade in human airway epithelial cells

et al. 2008

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Up-regulation of intercellular adhesion molecule-1 (ICAM-1) in the lung airway epithelium is associated with the epithelium-leukocyte interaction, critical for the pathogenesis of various lung airway inflammatory diseases such as asthma. However, little is known about how ICAM-1 is up-regulated in human airway epithelial cells. In this study, we show that tumor TNF-α induces monocyte adhesion to A549 human lung airway epithelium and also up-regulation of ICAM-1 expression. These effects were significantly diminished by pre-treatment with diphenyliodonium (DPI), an inhibitor of NADPH oxidase-like flavoenzyme. In addition, the level of reactive oxygen species (ROS) was increased in response to TNF-α in A549 cells, suggesting a potential role of ROS in the TNF-α-induced signaling to ICAM-1 expression and monocyte adhesion to airway epithelium. Further, we found out that expression of Rac

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“…Further studies showed by in situ hybridisation that expression of BLT2 is significantly upregulated in a variety of human cancers (Yoo et al, 2004). In addition, it has been suggested that BLT2 is responsible for LTB 4 -induced generation of reactive oxygen species (ROS) through Rac/ERK and that this receptor is involved in cytokineinduced differentiation and expansion of haematopoietic stem cells (Woo et al, 2002;Chung et al, 2005). Stem cell factor and TNF-a are suggested to regulate BLT2 expression (Lundeen et al, 2006;Qiu et al, 2006).…”

mentioning

confidence: 99%

BLT2 is expressed in PanINs, IPMNs, pancreatic cancer and stimulates tumour cell proliferation

et al. 2008

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Pancreatic cancer has an abysmal prognosis. Targets for early detection, prevention and therapy are desperately needed. Inflammatory pathways have an important impact on tumour growth and progression. Expression of BLT2 (the second leukotriene B 4 receptor) was investigated by real-time RT -PCR and immunohistochemistry. Cell proliferation was studied after stable transfection with BLT2, after treatment with siRNA and Compound A as an agonist. BLT2 is expressed in all pancreatic cancer cell lines. Results from real-time RT -PCR revealed significant overexpression of BLT2 in malignant intraductal papillary mucinous neoplasias (IPMNs) and pancreatic adenocarcinoma. Intense staining was evident in IPMNs, infiltrating tumour cells and advanced pancreatic intraepithelial neoplasias (PanINs) but not in normal ductal cells. Overexpression of BLT2 as well as stimulation of Colo357, Panc-1 and AsPC1 cells with Compound A caused a significant increase in tumour cell proliferation, an effect reversed after siRNA treatment. This study demonstrates for the first time the expression of BLT2 in the pancreas and overexpression in pancreatic cancers and malignant IPMNs in particular. Upregulation of BLT2 is already evident in precursor lesions (PanINs, IPMNs). Overexpression of this receptor leads to significant growth stimulation. Therefore, we suggest BLT2 as a new target for chemoprevention and therapy for pancreatic cancer.

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Leukotriene B4 pathway regulates the fate of the hematopoietic stem cells

Cited by 37 publications

References 13 publications

Linoleic Acid Induces Mouse Embryonic Stem Cell Proliferation Via Ca<sup>2+</sup>/PKC, PI3K/Akt, and MAPKs

Linoleic Acid Induces Mouse Embryonic Stem Cell Proliferation Via Ca<sup>2+</sup>/PKC, PI3K/Akt, and MAPKs

TNF-α-induced up-regulation of intercellular adhesion molecule-1 is regulated by a Rac-ROS-dependent cascade in human airway epithelial cells

BLT2 is expressed in PanINs, IPMNs, pancreatic cancer and stimulates tumour cell proliferation

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