Leukocytic Polypeptide Wound Hormones: Phenotype-Selective Chemorecruitment of Bone Marrow Leukocytes into Blood Circulation (Leukocytosis-Leukopenia-Leftward Shift Reactions)

Wissler, Josef H.; Renner, H.W.; Gerlach, U; Logemann, Enno

doi:10.1016/b978-0-08-031739-7.50284-6

Cited by 2 publications

(48 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The applied amounts were calculated on an average, not monoisotopic molecular mass of 35'075 Da of protonized form of CuRNP; it consists of copper-complexed 75-mer ARNA and ARP, as derived in results section and Fig. It was obtained by purification and removal of other bioactive cell-derived factors from supernatants of cultures of isolated porcine peripheral leukocytes, isolated mononuclear phenotypes or monocytes/macrophages maintained in serum-free, fully synthetic medium and activated with concanavalin A as lectin mitogen, as described in detail in [4,5,10,12,95]. For referencing moles to masses and multiples derived from that, 1 femtomol are 35 picogram pCuRNP N75-pARP .…”

Section: Methodsmentioning

confidence: 99%

“…7A. Biotechnical details on used conventional bioreactors and those specifically designed for control of defined hydrodynamics were also described [4,5,10,12,95]. It was obtained by purification and removal of other bioactive cell-derived factors from supernatants of cultures of isolated porcine peripheral leukocytes, isolated mononuclear phenotypes or monocytes/macrophages maintained in serum-free, fully synthetic medium and activated with concanavalin A as lectin mitogen, as described in detail in [4,5,10,12,95].…”

Section: Methodsmentioning

confidence: 99%

“…Chemical and biochemical analysis of proteins, nucleic acids, bioinorganic and bioorganic components are described in [10,12]. Enzymatic and chemohydrolysis of protein and RNA were performed as compiled in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Enzymatic and chemohydrolysis of protein and RNA were performed as compiled in Fig. 10 and in [12]. 10 and in [12].…”

Section: Methodsmentioning

confidence: 99%

“…Tissue remodeling subject to angiogenesis may lead to healing or chaotic processes; it includes sprouting and capillarization phases and development of closed vessel loops, as shown triggered by leukocytic angiotropin morphogens in this issue. Leukocytic angiotropins may be considered involved by their effects on arterial endothelial cells [4,5,9,10,12,37,38,83]. Thus, juxtracrine/paracrine signaling mechanisms may be considered predominant mechanisms effective in adhesion, diapedesis, locomotion and migration, i. e. chemokinesis and chemotaxis or haptotaxis of leukocytes/mononuclear cells into tissue [4, 9, 10, 125, 127, 128, 177 ± 179].…”

Section: Paradigm: Morphogenesis and Growth In Vascular Remodelingmentioning

confidence: 99%

See 4 more Smart Citations

Engineering of Blood Vessel Patterns by Angio-Morphogens [Angiotropins]:. Non-Mitogenic Copper-Ribonucleoprotein Cytokines [CuRNP Ribokines] with their Metalloregulated Constituents of RAGE-binding S100-EF-Hand Proteins and extracellular RNA Bioaptamers in Vascular Remodeling of Tissue and Angiogenesis in vitro

Wissler¹

2001

Mat.-wiss. u. Werkstofftech.

160

View full text Add to dashboard Cite

Tissue vascularization is requisite to successful cell‐based therapies, biomaterial design and implant integration. Thus, known problems in ossointegration of avascular implants in connection with the generation of bone tissue reflect arrays of general problems of socio‐economic relevance existing in reparative medicine still waiting for to be solved. For this purpose, morphogenesis and remodeling of endothelial angio‐architectures in tissue and in vitro by isolated non‐mitogenic angio‐morphogens [angiotropins] are considered in terms of their structure, function and action mechanisms. Extracellular angiotropins are secreted by activated leukocytes/monocytes/macrophages. They are a family of cytokines with morphogen bioactivity selectively directed to endothelial cells. Their structure was deciphered as metalloregulated copper‐ribonucleoproteins [CuRNP ribokines]. They are built up of angiotropin‐related S100‐EF‐hand protein [ARP] and highly modified and edited 5'end‐phosphorylated RNA [ARNA], complexed together by copper ions. Oxidant‐sensitive ARNA and their precursors represent novel types in a RNA world: They are the first isolated and sequenced forms of extracellular RNA [eRNA], may act as cytokine and bioaptamer, contain isoguanosine [crotonoside] as modified nucleoside and show up copper as RNA‐structuring transition metal ion. By metalloregulated bioaptamer functions, ARNA impart novel biofunctions to RAGE‐binding S100‐EF‐hand proteins. Angiotropin morphogens were shown suitable for neointiation and remodeling of blood vessel patterns in different, adult, embryonal and artificial tissues. These neovascular patterns manifest regulated hemodynamics for preventing tissue necrosis, supporting tissue functions and promoting wound healing. As evaluated in skin and muscle vascularization, the neovascular patterns are integrated into homeostatic control mechanisms of tissue. Thus, the morphogens show up beneficial perspectives and are suggested useful tools for further investigations in angiotherapy, engineering of blood vessel patterns in tissues and biocompatible artificial organs as well as in the preparation of novel implants with morphogen‐coated surfaces.

show abstract

Section: Methodsmentioning

confidence: 99%