Leukocytes Can Enhance Platelet-mediated Aggregation and Thromboxane Release <i>via</i>  Interaction of P-selectin Glycoprotein Ligand 1 with P-selectin

Faraday, Nauder; Scharpf, Robert B.; Dodd‐o, Jeffrey M.; Martinez, Elizabeth A.; Rosenfeld, Brian A.; Dorman, Todd

doi:10.1097/00000542-200101000-00025

Cited by 67 publications

(49 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8,9 Shear stress can also cause leukocyte activation in vitro, 8,9 which can in turn promote platelet activation. 41 However, it is unclear whether platelet or leukocyte activation is separately or sequentially up-regulated across coronary stenoses. Results from the in vitro arm of this study show that CD62P blockade completely suppressed shear-induced platelet-monocyte aggregation with no effect on shear-induced monocyte CD11b expression (Figure 10).…”

Section: Discussionmentioning

confidence: 99%

Intracoronary shear-related up-regulation of platelet P-selectin and platelet-monocyte aggregation despite the use of aspirin and clopidogrel

Yong

Pennings

Chang

et al. 2011

Blood

View full text Add to dashboard Cite

Recent in vitro studies have shown that shear stress can cause platelet activation by agonist-independent pathways. However, no studies have assessed the extent of shear-induced platelet activation within human coronary arteries. We sampled blood from the coronary arteries proximal and distal to coronary lesions and from the coronary sinus in humans with stable coronary disease who were taking both aspirin and clopidogrel. A novel, computationally based technique for estimating shear stress from 3-dimensional coronary angiographic images of these arteries was developed, and the effect of stenosis severity and calculated shear stress on in vivo platelet and related leukocyte activation pathways were determined. We provide evidence of intracoronary upregulation of platelet P-selectin, plateletmonocyte aggregation, and monocyte CD11b without platelet glycoprotein IIb-IIIa activation or soluble P-selectin up-regulation. This correlates with intracoronary stenosis severity and calculated shear stress and occurs despite the concurrent use of aspirin and clopidogrel. Our results show for the first time shear-related platelet and monocyte activation in human coronary arteries and suggest this as a potential therapeutic target that is resistant to conventional antiplatelet agents. (Blood. 2011;117(1): 11-20) IntroductionPlatelet activation and aggregation are critical to the pathogenesis of atherothrombosis. 1,2 Platelets are also known to play a main role in inflammation, partly by the interaction between platelets and leukocytes, 2-4 and it is known that shear stress can cause platelet activation, 5-12 platelet-leukocyte aggregation, 8,9 and leukocyte activation. 8,9 Procedures such as coronary angioplasty can alleviate focal coronary stenosis. However, many patients with diffuse, severe coronary disease are unsuitable for such treatment and may therefore be exposed to long-term platelet and leukocyte activation secondary to increased intracoronary shear stress.Although in vitro and animal studies have shown shear stressinduced platelet activation and aggregation, a direct relationship between shear stress and in situ platelet activation has not been shown within human coronary arteries. 13 Previous investigators have found evidence of transcardiac platelet activation with increased P-selectin expression as platelets travel from the aortic root to the coronary sinus (CS), the main vein draining blood from the heart in patients with coronary disease. 14 However, it is not known whether platelets are indeed activated as they cross coronary lesions, and no studies to date have investigated the direct effect of stenosis severity and shear stress on in situ platelet activation within human coronary arteries.Current antiplatelet agents such as aspirin, thienopyridines, and glycoprotein (GP) IIb-IIIa inhibitors attenuate agonist-induced platelet activation, [15][16][17] and GPIIb-IIIa inhibitors reduce shearinduced platelet aggregation. 10,18,19 However, shear stress overcomes aspirin inhibition of platelet aggregation. 20 Fu...

show abstract

Section: Discussionmentioning

confidence: 99%

Intracoronary shear-related up-regulation of platelet P-selectin and platelet-monocyte aggregation despite the use of aspirin and clopidogrel

Yong

Pennings

Chang

et al. 2011

Blood

View full text Add to dashboard Cite

show abstract

“…According to Faraday, et al this effect can be explained by the interaction of platelet P-selectin with leukocyte P-selectin glycoprotein ligand-1 leading to an enhanced platelet agonist-induced aggregation and thromboxane release [22].…”

Section: Discussionmentioning

confidence: 99%

Multiple electrode aggregometry in thrombocytopenic haemato-oncologic patients – Influence of haematologic variables

Opheim¹,

Apelseth²,

Stanworth³

et al. 2017

Hematol Med Oncol

View full text Add to dashboard Cite

show abstract

“…A variety of chemokines and leukocyte interactions via surface receptors have been reported to modulate platelet function and contribute to activation of platelets. [25][26][27] Theoretically, such a marked systemic inflammatory activity might be further increased by bile duct stenosis, malignancy, and radiotherapy. However, there is no convincing evidence that these mechanisms really have a clinical impact post transplant.…”

Section: See Article On Page 1372mentioning

confidence: 99%

The perspective of liver transplantation for cholangiocarcinoma

Jonas

Neuhaus

2007

Liver Transpl

View full text Add to dashboard Cite

Leukocytes Can Enhance Platelet-mediated Aggregation and Thromboxane Release via Interaction of P-selectin Glycoprotein Ligand 1 with P-selectin

Abstract: Leukocytes can enhance platelet agonist--induced aggregation and thromboxane release in whole blood and platelet-rich plasma under shear conditions in vitro. Interaction of platelet P-selectin with leukocyte PSGL1 contributes substantially to these effects.

Cited by 67 publications

References 0 publications

Intracoronary shear-related up-regulation of platelet P-selectin and platelet-monocyte aggregation despite the use of aspirin and clopidogrel

Intracoronary shear-related up-regulation of platelet P-selectin and platelet-monocyte aggregation despite the use of aspirin and clopidogrel

Multiple electrode aggregometry in thrombocytopenic haemato-oncologic patients – Influence of haematologic variables

The perspective of liver transplantation for cholangiocarcinoma

Contact Info

Product

Resources

About