Leukocyte migration inhibition in progressive systemic sclerosis

Hughes, Pamela J.; Holt, Shirley; Rowell, N.R.

doi:10.1111/j.1365-2133.1974.tb06709.x

Cited by 21 publications

(11 citation statements)

References 13 publications

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“…Although the inflammatory dermal infiltrate of scleroderma has been partially characterized (2,3, IS), the stimulus for this infiltrate is unknown. Several groups of investigators (5)(6)(7)19) have observed cellular immunity to collagen, skin extracts, and certain tissue antigens.…”

Section: Discussionmentioning

confidence: 99%

Cellular immunity to collagen and laminin in scleroderma

Huffstutter

DeLustro

LeRoy

1985

Arthritis & Rheumatism

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Peripheral blood mononuclear cells (PBMC) obtained from scleroderma patients and normal controls were cultured in the presence of types I and IV collagen, intima collagen, laminin, and phytohemagglutinin. PBMC from 9 of 19 patients (47%) demonstrated lymphocyte transformation in response to laminin, and those from 2 of 17 patients (12%) demonstrated reactivity to type IV collagen. None of the patient PBMC were responsive to type I collagen or to intima collagen. Healthy controls failed to demonstrate lymphocyte transformation in response to laminin or collagens. Analysis of clinical data, including age, disease duration, and degree of skin thickening and internal organ invollvement, failed to predict the response to laminin or type IV collagen. The significance and disease specificity of this cellular immunity to laminin and, with lower frequency, to type IV collagen, that occurs in some scleroderma patients are unknown.Scleroderma is a fibrosing disease of unknown etiology characterized by several immunologic abnor-

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Section: Discussionmentioning

confidence: 99%

Cellular immunity to collagen and laminin in scleroderma

Huffstutter

DeLustro

LeRoy

1985

Arthritis & Rheumatism

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“…Controls consisted of nine patients with rheumatoid arthritis, three with systemic lupus erythematosis (each fulfilling diagnostic criteria of the American Rheumatism Association), one patient with mixed connective tissue disease (19,20) and 16 Migration inhibition test. 25-30 ml of sterile mineral oil was injected into the peritoneal cavity of normal guinea pigs.…”

Section: Methodsmentioning

confidence: 99%

“…Migration inhibition was considered to be positive when it was less than 2 SD ofthe average migration inhibition ofthe 16 - The cells were incubated at 37°C for 24-72 h. The supernatants were then removed from each well and the wells washed once with 1 ml of medium 199. To each well was added 1 ml of 2% Triton X-100 (Rohm & Haas Co., Philadelphia, Pa.) and after 24 h the Triton was removed and the wells were washed once with 1 ml of water.…”

Section: Methodsmentioning

confidence: 99%

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Cutaneous antigen-stimulating lymphokine production by lymphocytes of patients with progressive systemic sclerosis (scleroderma).

Kondo¹,

Rabin²,

Rodnan³

1976

J. Clin. Invest.

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“…While T uffanelli [7] showed an increase in reactivity to leukocytes on skin testing in scleroderma, Fardal and W in k elm a n n [8] found cutaneous DNA sensitivity normal. Changes in migratory inhibition factor and lymphocyte toxicity have been noted in scleroderma [9], but lymphocyte transformation has been found to be normal [10].…”

Section: Commentmentioning

confidence: 99%

Peripheral Blood Lymphocyte Distribution in Scleroderma

Carapeto¹,

Winkelmann²

1975

Dermatology

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Determination in peripheral blood T and B lymphocytes performed in progressive systemic scleroderma (8 patients); dermatomyositis (5 patients); and inflammatory or mesenchymal scleroderma (‘mixed’ connective tissue disease) (5 patients) demonstrated a marked increase in peripheral B cells with T cell depression in patients with mesenchymal scleroderma. Patients with progressive systemic scleroderma and dermatomyositis demonstrated also a peripheral T depression, but no consistent changes in B cell population were found. The clear relationship between a B lymphocyte increase and elevated immunoglobulins, antinuclear and antiribonucleoprotein antibody and positive direct immunofluorescence of skin (basal membrane and blood vessels) defines inflammatory or mesenchymal scleroderma in which a disturbance in humoral immunity is involved. B lymphocyte determination can help to measure these changes in immunoreactivity.

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Leukocyte migration inhibition in progressive systemic sclerosis

Cited by 21 publications

References 13 publications

Cellular immunity to collagen and laminin in scleroderma

Cellular immunity to collagen and laminin in scleroderma

Cutaneous antigen-stimulating lymphokine production by lymphocytes of patients with progressive systemic sclerosis (scleroderma).

Peripheral Blood Lymphocyte Distribution in Scleroderma

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