Leukocyte endothelial cell interactions in pig to human organ xenograft rejection

Holgersson, Jan; Ehrnfelt, Cecilia; Hauzenberger, Elenor; Serrander, Lena

doi:10.1016/s0165-2427(02)00094-6

Cited by 16 publications

(14 citation statements)

References 47 publications

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“…In the flow-chamber assay, the binding of human neutrophils to PAECs treated with anti-␣-Gal Abs for 30 minutes and then incubated in medium for another 6 hours was significantly higher than to PAECs treated only with medium (PϽ0.01) (Fig. 2C) (22). Anti-␣-Gal Abs had no effect on neutrophil binding to HAECs.…”

Section: The Effect Of Anti-␣-gal Abs On the Adhesion Of Human Naive mentioning

confidence: 88%

Porcine endothelium activated by anti-α-GAL antibody binding mediates increased human neutrophil adhesion under flow

2003

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Our data suggest that anti-alpha-Gal Abs play an important role in the initiation of AVR by mediating adhesion and recruitment of neutrophils within an organ xenograft. In contrast with previous investigations, our data argues against a differential recognition of PAECs and HAECs by human neutrophils. Thus, to prevent AVR and accomplish long-term xenograft survival, it will be important to remove anti-alpha-Gal Abs before and after pig-to-human transplantation.

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Section: The Effect Of Anti-␣-gal Abs On the Adhesion Of Human Naive mentioning

confidence: 88%

Porcine endothelium activated by anti-α-GAL antibody binding mediates increased human neutrophil adhesion under flow

2003

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“…17 Xenotransplant (ie, transplant of tissue between different species) is considered a promising possible solution to overcome this problem. 18 One of the most challenging issues regarding xenotransplant as the treatment choice is immunologic rejection.…”

Section: Discussionmentioning

confidence: 99%

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Abdolghafoorian

Farnia²,

Rs³

et al. 2017

Exp Clin Transplant

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Objectives: Endothelial cells harbor many antigenic determinants that may be targets for the immune system. The aim of this study was to determine the immunologic effects of decellularization, using 3 different methods, on xenograft rejection. Materials and Methods: In a sterile plate containing phosphate-buffered saline, fresh sheep aortic heart valves were decellularized using 3 different enzymatic methods: with 900 µg/mL of collagenase at 40°C (method A), with 450 µg/mL of collagenase at 4°C (method B), and with 900 µg/mL of collagenase at 4°C (method C). Intact and decellularized valves were implanted subdermally into inbred male albino rabbits and extracted after 21 days (extra valve pieces were also extracted after 60 days, as control samples, for assessing chronic rejection). Valves were histologically analyzed for inflammatory cell infiltration. Subendothelial structure integrity was determined using surface electron microscope. Results: No inflammatory cell infiltration was seen around the decellularized valve with method A, and no subendothelial structure change was observed by surface electron microscope. Infiltration of immune cells involved in rejection was not seen around valves decellularized with method B, although the subendothelial structure was relatively preserved and valve stiffness was increased. With method C, we observed a foreign body-type reaction around the intact valve and the decellularized valve. Conclusions: Method A is considered the optimal method of decellularization in our study, as this method significantly reduced the immune response to xenograft tissue, while maintaining subendothelial tissue.

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“…For each of these, recruitment, adhesion, and trans-endothelial migration are mediated by different receptor-ligand interactions and finely regulated by chemotactic cytokines and chemokines released by host cells and the activated endothelium following xenotransplantation (Holgersson et al 2002). Compatibility between human receptors and their porcine counterparts is therefore indispensable for the optimal migration and function of each of these cells.…”

Section: The Role Of the Cells Involved In The Innate Immune Responsementioning

confidence: 99%

Immunological Challenges and Therapies in Xenotransplantation

Vadori

2014

Cold Spring Harbor Perspectives in Medicine

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Leukocyte endothelial cell interactions in pig to human organ xenograft rejection

Cited by 16 publications

References 47 publications

Porcine endothelium activated by anti-α-GAL antibody binding mediates increased human neutrophil adhesion under flow

Porcine endothelium activated by anti-α-GAL antibody binding mediates increased human neutrophil adhesion under flow

Untitled

Immunological Challenges and Therapies in Xenotransplantation

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