Leukocyte deformability is a novel biomarker to reflect sepsis‐induced disseminated intravascular coagulation

Morikawa, Miki; Inoue, Yoshiaki; Sumi, Yasuo; Kuroda, Yoko; Tanaka, Hiroshi

doi:10.1002/ams2.54

Cited by 4 publications

(7 citation statements)

References 26 publications

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“…Thus, larger gap sizes (e.g., 9-15 µm) are suitable for capturing other cells such as neutrophils, monocytes, and eosinophils, which are greater than 12 µm in size. Considering that cell deformability reflects its state and characteristics [2], the images of the captured cells in the device (i.e., images of cell distribution) could be used as a deformability profile of the sample. The deformability profile is dependent on the sample flow speed and gap sizes of the microfiltration device.…”

Section: Resultsmentioning

confidence: 99%

“…1 School of Mechatronic Engineering, Universiti Malaysia Perlis, Arau, Malaysia. 2 Institute of Innovation for Future Society, Nagoya University, Nagoya, Japan.…”

Section: Authors' Contributions Amn and Tm Performed Experiments And mentioning

confidence: 99%

“…These cells have the ability to deform and change their shapes when passing through a very small area, although the cell sizes are more significant than the area sizes [1]. Previous studies have found that the deformability of the leukocytes changes due to disease-related conditions, and they become more stiff (i.e., they are less deformable in patients suffering from diseases such as sepsis-induced disseminated intravascular coagulopathy [2], leukemia (chronic lymphocytic leukemia and acute lymphocytic leukemia) [3], diabetes mellitus [4], Epstein-Barr virus, and acute lung injury [5]). A number of studies have reported that diseases such as cancer (particularly lung and breast cancers) affect the deformability of cells (metastatic cells, in particular) depending on the stage of the tumor [6,7].…”

Section: Introductionmentioning

confidence: 99%

“…By understanding these properties, essential information may be obtained for further cell rheology studies crucial to understanding disease and improving health. Additionally, the mechanical properties of the cells, such as elasticity, shear characteristics, compression, and deformability, reflect their states and functions and may be used as biophysical markers for the detection of pathological cell changes [2]. Therefore, the analysis of the cell mechanical properties related to disease and drug treatments has attracted much attention [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

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Microfluidic device for rapid investigation of the deformability of leukocytes in whole blood samples

2020

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The mechanical properties of cells, such as leukocytes, in a diseased state differ from those of healthy cells, typically due to their microstructure. The deformability of the cells through a constrictive area is analyzed by the applied stress to the cell. This study investigates the relationship between the sample flow speed and distribution of captured leukocytes based on the cell deformability using a microfluidic device. The device comprises of microfilters that serve as the filtration mechanism. The microfilter gap size gradually decreases from 15 to 3 µm to facilitate the deformabilitybased separation. Leukocytes have various sizes; hence, they can be separated by microfilters directly from whole blood samples without any cell clogging, and they do not require sample pre-processing such as centrifugation or red blood cell lysis. The distribution of leukocytes captured by the microfilters with respect to the sample flow speed can be analyzed; at higher sample flow speeds of 6 µL/min, small leukocytes with a size of 7 µm could not be captured and they passed through the smallest microfilter gap size of 3 µm. For smaller leukocytes, such as lymphocytes, the distributions are mainly at gap sizes of 4 µm to 8 µm, with most of the lymphocytes captured at the 6 µm microfilter gap size. We conclude that the distribution of the cells captured during the filtration varies depending on the microfilter gap sizes, applied sample flow speed, cell sizes, and the ability of the cells to deform. The deformability imaging profiles of the sample could be developed from the images of the cell distribution, which might be useful for preliminary screening in the clinical applications. This work presents the development of a simple device for the study of cell deformability as the results provide a biophysical marker in high throughput and bulk sample analyses.

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Section: Resultsmentioning

confidence: 99%

“…1 School of Mechatronic Engineering, Universiti Malaysia Perlis, Arau, Malaysia. 2 Institute of Innovation for Future Society, Nagoya University, Nagoya, Japan.…”

Section: Authors' Contributions Amn and Tm Performed Experiments And mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microfluidic device for rapid investigation of the deformability of leukocytes in whole blood samples

2020

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“…Indeed, there can be differences in elastic moduli between cells of the same type, with a change in cell E having previously been shown to indicate disease progression (30). Malaria causes an increase in the shear elastic modulus of the membrane of RBCs (31,32), and leukocyte elasticity increases in patients diagnosed with sepsis (33,34). Conversely, decreased cancer cell elastic modulus is associated with increasing metastatic capabilities (35)(36)(37)(38).…”

Section: Introductionmentioning

confidence: 99%