Leukemia serum reactive with retrovirus‐related antigen in normal human placenta

Maeda, Shuichi; Yonezawa, Kazuya; Yoshizaki, Haruyasu; Mori, Masamitu; Kobayashi, Takeaki; Akahonai, Yoshikazu; Yachi, Akira; Mellors, Robert C.

doi:10.1002/ijc.2910380303

Cited by 13 publications

(5 citation statements)

References 19 publications

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“…Rabson et al (1985) detected transcripts of the env gene of clone 4-1 in normal human placenta. Maeda et al (1986) also reported that antiserum reactive with the human endogenous retrovirus p30 reacted with syncytiotrophoblasts. The reagent prepared for our study identified a molecule of 38 kDa in placental syncytiotrophoblasts.…”

Section: Discussionmentioning

confidence: 99%

Expression of an endogenous retroviral gene product in human placenta

Kitamura

Maruyama

Shirasawa

et al. 1994

Intl Journal of Cancer

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To investigate the presence and potential pathophysiological role of endogenous retroviruses in humans, we prepared a recombinant protein using clone 4-I, a proviral sequence. DNA fragments containing the env region of clone 4-I were subcloned into a prokaryotic expression vector (pET3), and 2 fusion proteins, SU413 and SU415, were then expressed in Escherichia coli after treatment with isopropyl-beta-thiogalactopyranoside (IPTG). By sonicating lysates of the transformed E. coli, the recombinant protein SU413 was successfully separated from the native bacterial components, and was used to raise an antiserum in rabbits. In immunoblot analysis, this antiserum specifically recognized the recombinant protein, but did not react with other components of E. coli. This antiserum was then used for an immunofluorescence study of human placenta, in which the env gene transcript has been reported. As a result, the anti-SU413 serum detected substances in syncytiotrophoblasts and vascular endothelia from a human placenta. No such reactivity was detected in human kidney or human liver. Immunoblot analysis revealed that this antiserum reacted to a single molecule of 38-kDa in placenta, and its reactivity was reduced by the antiserum absorbed with SU413 antigen. These findings suggest that human placental syncytiotrophoblasts and vascular endothelia preferentially express a molecule encoded by human endogenous retrovirus clone 4-I.

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Section: Discussionmentioning

confidence: 99%

Expression of an endogenous retroviral gene product in human placenta

Kitamura

Maruyama

Shirasawa

et al. 1994

Intl Journal of Cancer

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“…30,000 Da ''physiochemically similar to reference murine and primate type C retrovirus p30s and antigenically cross-reactive with p30 of the simian sarcoma-associated virus and gibbon ape leukemia virus primate retrovirus group.'' In a subsequent study, Maeda et al (114) reported that sera obtained from patients with acute leukemia and malignant lymphoma contained an immunoglobulin M antibody that reacted with placental syncytiotrophoblasts. It occurred less frequently in the sera of pregnant women and healthy controls.…”

Section: Expression Of Herv Antigens In Placental Tissuesmentioning

confidence: 97%

Section: Expression Of Herv Antigens In Placental Tissuesmentioning

confidence: 99%

Human endogenous retroviruses: nature, occurrence, and clinical implications in human disease

Urnovitz¹,

Murphy²

1996

Clin Microbiol Rev

175

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Retroviral diagnostics have become standard in human laboratory medicine. While current emphasis is placed on the human exogenous viruses (human immunodeficiency virus and human T-cell leukemia virus), evidence implicating human endogenous retroviruses (HERVs) in various human disease entities continues to mount. Literature on the occurrence of HERVs in human tissues and cells was analyzed. Substantial evidence documents that retrovirus particles were clearly demonstrable in various tissues and cells in both health and disease and were abundant in the placenta and that their occurrence could be implicated in some of the reproductive diseases. The characteristics of HERVs are summarized, mechanisms of replication and regulation are outlined, and the consistent hormonal responsiveness of HERVs is noted. Clear evidence implicating HERV gene products as participants in glomerulonephritis in some cases of systemic lupus erythematosus is adduced. Data implicating HERVs as etiologic factors in reproductive diseases, in some of the autoimmune diseases, in some forms of rheumatoid arthritis and connective tissue disease, in psoriasis, and in some of the inflammatory neurologic diseases are reviewed. The current major needs are to improve methods for HERV detection, to identify the most appropriate HERV prototypes, and to develop diagnostic reagents so that the putative biologic and pathologic roles of HERVs can be better evaluated.

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“…It occurred less frequently in the sera of pregnant women and healthy controls. The main reactive antigen was reported to be a nonglycosylated 32-Da protein that was antigenically related to simian sarcoma-associated virus p30 (114). The studies of Kurth et al (97) revealed a very interesting pattern of antibodies to type C viral antigens in patients with testicular teratocarcinomas.…”

Section: Expression Of Herv Antigens In Placental Tissuesmentioning

confidence: 99%