Leukapheresis for autologous stem cell transplantation: Comparative study of two different thawing methods WSCFD® Stem Cell Fast Thawer KW versus 37 °C thermostatic bath

An alternative procedure to leukapheresis for peripheral hematopoietic progenitor cell collection in very‐low‐weight children: A single pediatric center experience

“…On transplant day, aliquots were thawed at 37 C and CD34 + enumeration and viability were assessed by flow cytometer according to ISHAGE single-platform protocol as previously described. 15,16 F I G U R E 1 Example of assembled "home-made" circuit for…”

“…Starting from the third day of GCSF stimulation, peripheral blood CD34 + cells were counted by FACS analysis as previously described. 15 The CD34 + cell count among total leukocytes was determined according to International Society of Hematotherapy and Graft Engineering (ISHAGE) single platform protocol. 16 Collections started when CD34 + cell count in patient's peripheral blood exceeded at least 200/μL.…”

Comparison of CryoMACS Freezing Bags with Maco Biotech Freezing-Ethinyl Vinyl Acetate Bags for Hematopoietic Progenitor Cells Cryopreservation Using a CD34+-Enriched Product

“…15 After freezing, bags were stored in the vapor phase of liquid nitrogen (LN 2 ) for 7 days. Finally, all bags were thawed using an automated method, 16 dried and checked for leakages/damages. To better detect leakages, bags were wrapped between paper tissue and an *2 kg weight was applied for 2 minutes.…”

“…Apheresis products were intended for disposal and research use with informed consent, according to international and Italian guidelines. 17,18 Apheresis plasma was quickly thawed using an automated method 16 and washed with saline (0.9% sodium chloride injection, USP, 100 mL VIAFLEX, Baxter) and 20% human serum albumin (Albital 200 g/L, Kedrion Biopharma) solution to remove DMSO and cellular debris. The starting number of CD34 + cells was estimated from the first cryopreservation step and later verified by flow cytometry as described hereunder.…”

“…After filling, bags were cryopreserved by controlled rate freezing (Kryo 550; Planer) 6 and stored in the vapor phase of LN 2 for at least 6 months. At the end of the 6 months of storage, bags were quickly thawed using a automated method as described before, 16 allowing standardized thawing times based on the filling volume.…”