Leucokinins, a new family of ion transport stimulators and inhibitors in insect Malpighian tubules

Hayes, Timothy K.; Pannabecker, Thomas L.; Hinckley, D.J.; Holman, G. Mark; Nachman, R.J.; Petzel, D. H.; Beyenbach, Klaus W.

doi:10.1016/0024-3205(89)90362-7

Cited by 177 publications

(97 citation statements)

References 15 publications

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“…Previous studies showed that kinin peptides have actions in the periphery, at the level of neuromuscular systems and effector organs (Coast, 1998;Hayes et al, 1989;Terhzaz et al, 1999;Torfs et al, 1999). In this paper, we show that pevkinin also modulates motor circuit activity within the crustacean nervous system.…”

Section: Discussionsupporting

confidence: 62%

“…Given the previously documented peripheral actions of kinin peptides in other arthropods (Coast, 1998;Hayes et al, 1989;Terhzaz et al, 1999;Torfs et al, 1999) and its neurohemal organ localization in C. borealis, it may well be that the PevKs further modify the pyloric motor system via peripheral actions. The C. borealis neuromuscular system is known to be extensively modulated, both pre-and post-synaptically (Jorge-Rivera and …”

Section: Pevkinin Actions On the Gastric Mill Circuitmentioning

confidence: 93%

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Actions of kinin peptides in the stomatogastric ganglion of the crabCancer borealis

Saideman

Christie

Torfs

et al. 2006

Journal of Experimental Biology

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Penaeus vannamei, but they have yet to be studied in the STG of any species. We identified kinin-like immunolabeling (KLI) in the pericardial organs (POs) in C. borealis, but there was no KLI within the STG. The POs are a major source of hormonal influence on the STG. Pevkinin peptides activated the pyloric circuit and they caused a modest increase in the speed of ongoing pyloric rhythms. This modest influence on cycle speed resulted in part from pevkinin excitation of the lateral pyloric neuron, whose strengthened inhibitory synapse onto the pyloric pacemaker neurons limited the pevkinin-mediated increase in cycle speed. The pevkinin excitation of the pyloric rhythm was not strong enough to interfere with the previously documented, gastric mill rhythm-mediated weakening of the pyloric rhythm. Pevkinin also had little influence on the gastric mill rhythm. These results indicate that the kinin peptides have distinct and selective modulatory actions on the pyloric rhythm.

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Section: Discussionsupporting

confidence: 62%

Section: Pevkinin Actions On the Gastric Mill Circuitmentioning

confidence: 93%

Actions of kinin peptides in the stomatogastric ganglion of the crabCancer borealis

Saideman

Christie

Torfs

et al. 2006

Journal of Experimental Biology

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“…Later, members of this family were found to have a role in gustatory and olfactory perception [74] and feeding behavior [75], and to act as ion transport regulators [76]. Drosophila leucokinin has been shown to be present in the brain and ventral ganglia [75].…”

Section: Fmrfamides -Leucokinin -Proctolinmentioning

confidence: 99%

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Caers

Boonen

Abbeele³

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Neuropeptides and peptide hormones are essential signaling molecules that regulate nearly all physiological processes. The recent release of the tsetse fly genome allowed the construction of a detailed in silico neuropeptide database (International Glossina Genome Consortium, Science 344, 380-386 (2014)), as well as an in-depth mass spectrometric analysis of the most important neuropeptidergic tissues of this medically and economically important insect species. Mass spectrometric confirmation of predicted peptides is a vital step in the functional characterization of neuropeptides, as in vivo peptides can be modified, cleaved, or even mispredicted. Using a nanoscale reversed phase liquid chromatography coupled to a Q Exactive Orbitrap mass spectrometer, we detected 51 putative bioactive neuropeptides encoded by 19 precursors: adipokinetic hormone (AKH) I and II, allatostatin A and B, capability/ pyrokinin (capa/PK), corazonin, calcitonin-like diuretic hormone (CT/DH), FMRFamide, hugin, leucokinin, myosuppressin, natalisin, neuropeptide-like precursor (NPLP) 1, orcokinin, pigment dispersing factor (PDF), RYamide, SIFamide, short neuropeptide F (sNPF) and tachykinin. In addition, propeptides, truncated and spacer peptides derived from seven additional precursors were found, and include the precursors of allatostatin C, crustacean cardioactive peptide, corticotropin releasing factor-like diuretic hormone (CRF/DH), ecdysis triggering hormone (ETH), ion transport peptide (ITP), neuropeptide F, and proctolin, respectively. The majority of the identified neuropeptides are present in the central nervous system, with only a limited number of peptides in the corpora cardiaca-corpora allata and midgut. Owing to the large number of identified peptides, this study can be used as a reference for comparative studies in other insects.

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“…They were found to influence transepithelial fluid secretion in isolated Malpighian tubules by increasing the Cl permeability of the tubule membrane. 47 Recently, a leucokinin-like peptide (NSVVLGKKQRFHSWGamide) was isolated and characterized from an extract of 400 g of whole adult fruit flies. This Drosophila leucokinin (DLK) stimulates secretion in D. melanogaster Malpighian tubules.…”

Section: The Leucokinin Precursormentioning

confidence: 99%

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

et al. 2005

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Peptides are the largest class of signalling molecules found in animals. Nevertheless, in most proteomic studies peptides are overlooked since they literally fall through the mazes of the net. In analogy with proteomics technology, where all proteins expressed in a cell or tissue are analyzed, the peptidomic approach aims at the simultaneous visualization and identification of the whole peptidome of a cell or tissue, i.e. all expressed peptides with their post-translational modifications. In this paper we describe the analysis of the larval fruit fly central nervous system using two-dimensional capillary liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/Q-TOF-MS/MS. Using the central nervous systems of only 50 larval Drosophila as starting material, we identified 38 peptides in a single analysis, 20 of which were not detected in a previous study that reported on the one-dimensional capillary LC/MS/MS analysis of the same tissue. Among the 38 sequenced peptides, some originate from precursors, such as the tachykinin and the IFamide precursor that were entirely missed in the first study. This clearly demonstrates that the two-dimensional capillary LC approach enhances the coverage of the peptidomic analysis.

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Leucokinins, a new family of ion transport stimulators and inhibitors in insect Malpighian tubules

Cited by 177 publications

References 15 publications

Actions of kinin peptides in the stomatogastric ganglion of the crabCancer borealis

Actions of kinin peptides in the stomatogastric ganglion of the crabCancer borealis

Peptidomics of Neuropeptidergic Tissues of the Tsetse FlyGlossina morsitans morsitans

Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two‐dimensional capillary liquid chromatography quadrupole time‐of‐flight mass spectrometry

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