Leucojum aestivum L. in vitro bulbs induction and acclimatization

Ptak, Agata

doi:10.2478/s11535-014-0339-5

Cited by 8 publications

(6 citation statements)

References 42 publications

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“…In terms of bulbing ability in bulbous plants, Rice et al (1983) enhanced tulip bulb production in vitro by soaking adventitious shoots developed into a bulb in GA 3 . In contrast, treatments with GA 3 in explant shoots strongly inhibited the bulb formation of tulips in a study reported by Podwyszy nska and Ross (2003), and the application of inhibitors of GA biosynthesis, such as paclobutrazol, had a positive effect on bulb formation in initial explants of some bulbous plants including Leucojum aestivum (Ptak, 2014) and tulip (Podwyszy nska, 2006). ABA was found to stimulate the formation of bulb in Hyacinthus orientalis (Li et al, 2002).…”

mentioning

confidence: 75%

Dynamic Changes in Starch Metabolism and Endogenous Hormones during Stolon Formation in Tulipa edulis

Miao

Zhu

et al. 2016

J. Amer. Soc. Hort. Sci.

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The stolon is the main asexual reproductive organ of the medicinal plant Tulipa edulis and has special morphology. In the greenhouse experiment presented herein, the dynamic changes in carbohydrates and related enzymes, proteins, and endogenous hormones of stolons during T. edulis stolon formation were investigated. The results showed that the content of total soluble sugar, sucrose, reducing sugar, fructose, and starch were all significantly enhanced in the middle period when stolon emerged and maintained at relatively high levels until the later period of stolon formation, while protein content decreased during stolon formation. The activities of amylase (AMY), sucrose phosphate synthase (SPS), and sucrose synthase (SS) peaked in the initial period and were negatively correlated with soluble sugars. However, adenosine diphosphoglucose pyrophosphorylase (AGPase) activity increased as stolon formation progressed, and the changes in soluble starch synthase (SSS), granule-bound starch synthase (GBSS) activities presented a single peak, reaching their maximums in the middle period. AGPase, SSS, and GBSS activities were all positively related to starch content. Moreover, quantitative real-time polymerase chain reaction (qRT-PCR) verified the changes in SS and SSS activities via the expression levels of the SS, SSSI, and SSSII genes. The gibberellin (GA) and zeatin riboside (ZR) content attained their maximum in the initial period of stolon formation. Indole-3-acetic acid (IAA) and abscisic acid (ABA) remained at high levels during the initial and middle period and decreased significantly during the later period of stolon formation, inversely to the ratio of ABA:IAA. Analysis of the physiological changes in T. edulis stolon indicated that the accumulation of soluble sugars and starch via various enzymes, a high level of IAA and a low ABA to IAA ratio mainly contributed to stolon development of T. edulis. This paper explored carbohydrate levels and endogenous hormones profiles during stolon formation, which provided the theory basis for further regulating stolon growth of T. edulis.

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confidence: 75%

Dynamic Changes in Starch Metabolism and Endogenous Hormones during Stolon Formation in Tulipa edulis

Miao

Zhu

et al. 2016

J. Amer. Soc. Hort. Sci.

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“…After 3 months of cultivation, plantlets were transferred to the same fresh medium. The formations of bulblets took place at 5°C in the darkness during 1 month according to the method previously described .…”

Section: Methodsmentioning

confidence: 99%

Stimulating effect of both 4’‐O‐methylnorbelladine feeding and temporary immersion conditions on galanthamine and lycorine production by Leucojum aestivum L. bulblets

Saliba

Ptak

Boisbrun

et al. 2016

Engineering in Life Sciences

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Bulb cultures of Leucojum aestivum and L. aestivum 'Gravety Giant' were subcultured in medium containing the precursor 4'-O-methylnorbelladine (MN) at various concentrations [0 (control), 0.15 and 0.3 g/L]. The cultures were conducted in bioreactor RITA R and lasted for 15, 30, 40 and 50 days. The growth rate and the alkaloid accumulation in bulblets were studied. For this latter purpose, a purification method was developed. It comprised a highly selective solid phase extraction using on the one hand, UPTI-CLEAN SI and SCX cartridges for plant extracts and on the other hand, 2H cartridges for culture media. Pure alkaloidal fractions were, thus, analyzed by LC-ESI-MS allowing the quantitative evaluation of galanthamine and lycorine from culture extracts. Precursor feeding along with temporary immersion conditions was found to significantly improve the accumulation of both galanthamine and lycorine. The maximal concentrations of galanthamine (0.81 mg/g DW) and lycorine (0.54 mg/g DW) in L. aestivum bulblets were reached, respectively, after 40 days of culture with 0.15 g/L of precursor and after 30 days of culture with 0.3 g/L of precursor. In L. aestivum 'Gravety Giant' bulb cultures, 0.3 g/L of precursor was the best condition for both galanthamine (0.6 mg/g DW after 50 days) and lycorine (1.13 mg/g DW after 30 days).

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“…The growth and size of bulb depend upon the distribution of carbohydrates in root and shoot of a plant. The supplementation of bulblets with sucrose and growth retardants like paclobutrazol exogenously in the culture medium affects the bulb size under in vitro conditions (Saos et al, 2002;Ptak et al, 2014). As a known inhibitor of gibberellin biosynthesis, paclobutrtazol retard shoot elongation and lead to an increase in the root-to-shoot ratio (bulb weight) by translocation of assimilates from the leaves to the storage organs in various bulbous and tuberous species (Li et al, 2014;Yan et al, 2011;Harvey et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. professor Blaauw and genetic fidelity studies using SCoT markers

Verma

Kumar

Priti

et al. 2022

Plant Cell Tiss Organ Cult

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Dutch Iris is a commercially important bulbous ornamental crop. Its high demand in global oriculture market necessitates the production of high-quality planting material. In the present investigation, the signi cance of meta-topolin (mT), a novel aromatic cytokinin, was studied on in vitro morphogenesis of Iris × hollandica Tub. cv. Professor Blaauw (Dutch Iris). Cytokinin mT (1.0 mg L − 1 ) as a better substitute to BAP (6-Benzylaminopurine) and Kn (Kinetin), has resulted in maximum shoot induction response (86.11 per cent) and increased emergence of micro shoots (4.95 shoots/explant with average shoot length of 5.65 cm) from twin scale explants. Photoperiod also affected the shoot induction, wherein initial 1-week (w) dark incubation signi cantly increased shoot induction response (94.44 per cent). Both meta-topolin and initial 1w dark incubation resulted in improved quality of regenerated shoots by reducing the incidence of necrosis and preventing vitri cation. The collective consequence of cytokininauxin (1.0 mg L − 1 mT + 0.25 mg L − 1 NAA) showed considerably higher number of shoots (17.53) with mean shoot length (6.90 cm) and maximum number of bulbets (2.74). Positive effect of increased sucrose concentration (90 g L 1 ) and paclobutrazol (5 mg L 1 ) on in vitro bulblet formation and bulblet size was also observed. The superiority of mT over BAP was also found during in vitro rhizogenesis. Shoots derived from mT medium were healthy and long enough, thus showed better rooting response (63.3 %) on ½ MS medium + 0.5 mg L − 1 IAA after 4 weeks. The mT raised plantlets were acclimatized in a greenhouse and showed 89.6 % survival under ex vitro conditions The clonal delity of the in vitro regenerated plants was evaluated using Start Codon Targeted (SCoT) markers. Out of 36 primers, 13 primers showed clearly scorable monomorphic bands, thus displaying genetic uniformity among in vitro regenerated plantlets. This meta-topolin mediated protocol can be routinely used for the rapid large scale production of this valuable oriculture crop.

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Leucojum aestivum L. in vitro bulbs induction and acclimatization

Abstract: Abbreviations:ANC -ancymidol; FW -fresh weight; GA 3 -gibberellic acid; LM -liquid medium; MS -Murashige and Skoog basal medium; PBZ -paclobutrazol; SCR -sucrose; SEM -scanning electron microscopy; SM -solid medium.

Cited by 8 publications

References 42 publications

Dynamic Changes in Starch Metabolism and Endogenous Hormones during Stolon Formation in Tulipa edulis

Dynamic Changes in Starch Metabolism and Endogenous Hormones during Stolon Formation in Tulipa edulis

Stimulating effect of both 4’‐O‐methylnorbelladine feeding and temporary immersion conditions on galanthamine and lycorine production by Leucojum aestivum L. bulblets

Meta-Topolin mediated in vitro propagation in an ornamentally important crop Iris × hollandica Tub. cv. professor Blaauw and genetic fidelity studies using SCoT markers

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