Leucocyte Separation and Transfusion

Goldman, JM

doi:10.1111/j.1365-2141.1974.tb00808.x

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…The interphase was fractionated by the MACS technique (9). Human leukemia or lymphoma cells were identified by morphological, cytochemical, and immunological criteria and were derived from diagnostic blood samples or therapeutic leukopheresis (10). Since the cell counts in the patients investigated were extremely high ( (9).…”

Section: Methodsmentioning

confidence: 99%

DNA methylation profiles in the human genes for tumor necrosis factors alpha and beta in subpopulations of leukocytes and in leukemias.

Kochanek

Radbruch

Tesch

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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The genomic sequencing technique has been applied to assess the state of methylation in the DNA from human leukocyte subpopulations from healthy individuals'and in the DNA from several individuals with myeloid or lymphatic leukemias or non-Hodgkin lymphomas. Leukocyte populations were purified by the high-gradient magnetic cell sorting' technique. In the human tumor necrosis factor a (TNF-a) gene segment between nucleotides 300 and 1150, the specific methylation profile in the DNA from human granulocytes and monocytes is maintained in three cases of myeloid leukemia. In one such case, all 5-methyl-2'-deoxycytidine residues' have been replaced by cytidine. In a chronic lymphatic T-cell leukemia, all 5-methyl-2'-deoxycytidine residues have been substituted by cytidine. In normal B lymphocytes, in two cases of chronic lymphatic B-cell leukemias and two cases of non-Hodgkin lymphomas, all 5'-CG-3' sequences in this gene segment are devoid of methylation. In the TNF-,B gene, DNA methylation is decreased in several examples of acute or chronic myeloid leukemias in comparison to normal human granulocytes or monocytes, whose DNA is almost completely methylated between nucleotides 700 and 900. In human T and B lymphocytes, the main producers of TNF-,B, in three instances of chronic lymphatic leukemias and two cases of non-Hodgkin Iymphomas, all 5'-CG-3' sequences are unmethylated in this region. The DNA from the human HeLa cell line is highly methylated at all 5'-CG-3' sequences in the TNF-a and -,f genes. The TNF-a gene is transcribed in the cells of one case of acute myeloid leukemia in which the analyzed region of the TNF-a gene is completely unmethylated. The TNF-8 gene is not transcribed in any of the malignant cells tested.Patterns of methylation in human DNA are specific for different cell types and can provide clues to states of genetic activities. By using the genomic sequencing technique (1, 2), we have shown that specific patterns of DNA methylation, in the human genes for tumor necrosis factors a and 8 (TNF-a and TNF-,B) (3) and in randomly selected sections of the human genome (4), are characterized by a high degree of interindividual concordance among people of different ethnic origins (3). Thus far, however, only a very small proportion of the human DNA has been analyzed. In other segments of the human genome, interindividual differences in methylation patterns have been reported (5, 6) and changes in human malignancies occur (7,8).We Fig. 1.Extraction of DNA and Genomic Sequencing. Nuclear DNA was extracted by the SDS/proteinase K/phenol method (11).The genomic sequencing method of Church and Gilbert (1) was applied with modifications (2, 12, 13). The TNF-a gene-containing fragment was excised from human DNA with Stu I and Bgl I and isolated by zone velocity sedimentation (3, 12). The DNA in the appropriate size fraction was recleaved with BstXI or Sty I (see Fig. 2a). Similarly, the TNF-,B gene was enriched, the initial cleavage being with Pvu II or BamHI followed by Hinfl (Fig. 3a). The presele...

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Section: Methodsmentioning

confidence: 99%

DNA methylation profiles in the human genes for tumor necrosis factors alpha and beta in subpopulations of leukocytes and in leukemias.

Kochanek

Radbruch

Tesch

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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Discontinuous Flow Centrifugation Leukapheresis and Neutrophil Function

Glasser

1977

Transfusion

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Some Aspects of Recent Advances in the Use of Blood Cell Components

Schiffer¹

1978

Br J Haematol

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Intensive research and major technical advances in the last decade have resulted in a remarkable improvement in transfusion supportive care which has significantly contributed to the increased survival now achievable in patients with leukaemia and aplastic anaemia. Platelet transfusions are now available in almost all hospitals, while granulocyte transfusions, previously used only in cancer centres, are now being dispensed by many regional blood transfusion centres in the United States. A number of reviews dealing with donor selection and safety, collection methodology and clinical guidelines for cell component use have been published in the last few years

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Leucocyte Separation and Transfusion

Cited by 3 publications

References 23 publications

DNA methylation profiles in the human genes for tumor necrosis factors alpha and beta in subpopulations of leukocytes and in leukemias.

DNA methylation profiles in the human genes for tumor necrosis factors alpha and beta in subpopulations of leukocytes and in leukemias.

Discontinuous Flow Centrifugation Leukapheresis and Neutrophil Function

Some Aspects of Recent Advances in the Use of Blood Cell Components

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