Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development

Gagné, Séverine; Lacampagne, Soizic; Claisse, Olivier; Gény, Laurence

doi:10.1016/j.plaphy.2008.12.004

Cited by 63 publications

(56 citation statements)

References 37 publications

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“…Here we could clearly see that PA levels were specifically increased in ft-1 siliques compared with wild type, with levels of alternative flavonoid end products at wild-type levels. Synthesis of PAs was previously believed to be exclusively in the inner integument of Arabidopsis seeds, but it has been detected previously in fruit tissues of grape (Vitis vinifera) (25). To determine how FT affects tannin synthesis during seed maturation, we profiled gene expression in seeds and siliques of wild type and ft-1 (Fig.…”

Section: Analysis Ofmentioning

confidence: 99%

Maternal temperature history activates Flowering Locus T in fruits to control progeny dormancy according to time of year

Chen

MacGregor

Dave

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

158

138

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Seasonal behavior is important for fitness in temperate environments but it is unclear how progeny gain their initial seasonal entrainment. Plants use temperature signals to measure time of year, and changes to life histories are therefore an important consequence of climate change. Here we show that in Arabidopsis the current and prior temperature experience of the mother plant is used to control germination of progeny seeds, via the activation of the florigen Flowering Locus T (FT) in fruit tissues. We demonstrate that maternal past and current temperature experience are transduced to the FT locus in silique phloem. In turn, FT controls seed dormancy through inhibition of proanthocyanidin synthesis in fruits, resulting in altered seed coat tannin content. Our data reveal that maternal temperature history is integrated through FT in the fruit to generate a metabolic signal that entrains the behavior of progeny seeds according to time of year.M any organisms use annual changes in temperature to control their phenology, resulting in predictable timing of key life history events, such as flowering, spawning, and migration (1-3). Understanding crop and ecosystem response to climate change requires knowledge of the temperature control of key developmental transitions, but how new generations achieve seasonal orientation is currently unclear. Seed germination is the first step in plant life history and therefore plays a central role in the control of plant phenology (4) and is extremely sensitive to environmental temperature (3-5). Seed dormancy is established during seed maturation and is imposed by control of hormone signaling and the action of the maternal seed coat. Nearly 30 y ago it was found that environmental signaling throughout the whole maternal life history can affect seed dormancy control in wild oats, and that temperature experience in the vegetative phase before flowering affected progeny seed dormancy (6). Here we show that this response is conserved on the model species Arabidopsis. Our data show that fruit tissues carry a memory of past temperature experience and that flowering pathways control a transgenerational metabolic signal of maternal past temperature experience, which modulates progeny dormancy according to time of year.To test whether past parental temperature experience affected progeny dormancy in the model species Arabidopsis thaliana, we grew plants until the first sign of flowering at either 22°C or 16°C and then placed plants side by side to set seed at 22°C in long days (LDs) (Fig. 1A). We found that in Landsberg erecta (Ler) lower temperature during the vegetative phase caused a large increase in the dormancy of seeds produced later on the plants (Fig. 1A). Lower temperatures during seed set also increase progeny dormancy (6), but we observed no effect of photoperiod on dormancy either before or after flowering, as has been reported previously (7, 8). Therefore, temperature signals before seed fertilization are remembered by the parent plant and used to control offspring behavior.P...

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Section: Analysis Ofmentioning

confidence: 99%

Maternal temperature history activates Flowering Locus T in fruits to control progeny dormancy according to time of year

Chen

MacGregor

Dave

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

158

138

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“…Despite these Wndings on basic PA regulatory mechanisms, few reports have shown the phenological response of these Myb-TFs to ambient conditions. A few reports on grapevine berry skin and seeds have demonstrated phenological expression patterns of VvMYBPA1, VvMYB5a, and 5b (Bogs et al 2007;Gagné et al 2009;Terrier et al 2009), and their response to light conditions (Matus et al 2009). Early progress in the study of Myb-TFs involved in anthocyanin biosynthesis and their utilization in the coloration of commercial plants, such as Rosaceae and Vitis spp., led to characterization of regulation of the anthocyanin biosynthesis pathway, which shares almost all enzymatic genes with the PA biosynthesis pathway (Fig.…”

Section: Introductionmentioning

confidence: 99%

Effects of seasonal temperature changes on DkMyb4 expression involved in proanthocyanidin regulation in two genotypes of persimmon (Diospyros kaki Thunb.) fruit

Akagi

Tsujimoto

Ikegami

et al. 2011

Planta

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Persimmon fruits accumulate a large amount of proanthocyanidin (PA). Fruits of the mutant non-astringent (NA) type lose their ability to accumulate PA at an early stage of fruit development, whereas fruits of the normal astringent (A) type sustain PA accumulation until ripening. This allelotype is determined by the genotype of a single ASTRINGENCY (AST) locus. It is possible that the reduction in PA accumulation in NA-type fruits is due to phenological down-regulation of DkMyb4 (a PA regulator) and the resultant down-regulation of structural genes in the PA pathway. In this study, attempts were made to identify the regulatory mechanisms of phenological PA accumulation in A- and NA-type fruits, focusing particularly on the effects of ambient temperature. Continuous cool temperature conditions caused sustained expression of DkMyb4 in NA-type fruits, as well as in A-type fruits, resulting in increased expression of PA pathway genes and PA accumulation. However, the expression of some A/NA phenotypic marker genes was not significantly affected by the cool temperature conditions. In addition, PA composition in NA-type fruits exposed to cool temperatures differed from that in A-type fruits. These results indicate that a cool ambient temperature may have induced DkMyb4 expression and resultant PA accumulation, but did not directly affect the expression of the AST gene.

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“…In contrast to the progress in the study of anthocyanin biosynthesis regulation, few reports have demonstrated the mechanisms that regulate PA biosynthesis in response to environmental factors, despite the fact that PA and anthocyanin pathways share almost all enzymatic genes. The seasonal expression patterns of three Myb TFs (VvMYBPA1, VvMYB5a, and VvMYB5b) and their response to light conditions were demonstrated in grape skin and seeds (Bogs et al, 2007;Gagné et al, 2009;Matus et al, 2009;Terrier et al, 2009). In general, certain biotic and abiotic stresses affect MYB134 expression levels in leaves of certain plants (Mellway et al, 2009).…”

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confidence: 99%

Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit

et al. 2011

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Proanthocyanidins (PAs) are secondary metabolites that contribute to plant protection and crop quality. Persimmon (Diospyros kaki) has a unique characteristic of accumulating large amounts of PAs, particularly in its fruit. Normal astringent-type and mutant nonastringent-type fruits show different PA accumulation patterns depending on the seasonal expression patterns of DkMyb4, which is a Myb transcription factor (TF) regulating many PA pathway genes in persimmon. In this study, attempts were made to identify the factors involved in DkMyb4 expression and the resultant PA accumulation in persimmon fruit. Treatment with abscisic acid (ABA) and an ABA biosynthesis inhibitor resulted in differential changes in the expression patterns of DkMyb4 and PA biosynthesis in astringent-type and nonastringent-type fruits depending on the development stage. To obtain an ABA-signaling TF, we isolated a full-length basic leucine zipper (bZIP) TF, DkbZIP5, which is highly expressed in persimmon fruit. We also showed that ectopic DkbZIP5 overexpression in persimmon calluses induced the up-regulation of DkMyb4 and the resultant PA biosynthesis. In addition, a detailed molecular characterization using the electrophoretic mobility shift assay and transient reporter assay indicated that DkbZIP5 recognized ABA-responsive elements in the promoter region of DkMyb4 and acted as a direct regulator of DkMyb4 in an ABA-dependent manner. These results suggest that ABA signals may be involved in PA biosynthesis in persimmon fruit via DkMyb4 activation by DkbZIP5.

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Leucoanthocyanidin reductase and anthocyanidin reductase gene expression and activity in flowers, young berries and skins of Vitis vinifera L. cv. Cabernet-Sauvignon during development

Cited by 63 publications

References 37 publications

Maternal temperature history activates Flowering Locus T in fruits to control progeny dormancy according to time of year

Maternal temperature history activates Flowering Locus T in fruits to control progeny dormancy according to time of year

Effects of seasonal temperature changes on DkMyb4 expression involved in proanthocyanidin regulation in two genotypes of persimmon (Diospyros kaki Thunb.) fruit

Seasonal Abscisic Acid Signal and a Basic Leucine Zipper Transcription Factor, DkbZIP5, Regulate Proanthocyanidin Biosynthesis in Persimmon Fruit

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