Leucine-Rich Diet and Light Aerobic Training Modulate GLUT4 Expression And Increase the Glycogen Content in the Gastrocnemius Muscle of Walker-256 Tumour-Bearing Rats

Salomão, Emilianne Miguel; Toneto, Aline T.; Silva, Gisele O.; Gomes-Marcondes, M.C.C.

doi:10.4172/2324-8793.1000112

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“…Therefore the higher Pygm found in W group likely was related to increased muscle glycogenolysis providing glucose for immediate use in glycolysis reactions. In contrast, the WL group could preserve the muscle glycogen (as previously showed [ 42 , 44 ] which could be in accordance with the maintenance of muscle energy source and consequently the muscle mass. Besides that, the Mdh1 and Mdh2 proteins that are related to mitochondrial catalytic activity and, together with the Pdhb protein, responsible for the conversion of pyruvate to Acetyl-CoA, showed high concentration in both groups supplemented with leucine, including the tumour-bearing group (L, WL).…”

Section: Discussionsupporting

confidence: 74%

Leucine-Rich Diet Modulates the Metabolomic and Proteomic Profile of Skeletal Muscle during Cancer Cachexia

Cruz

Oliveira

Viana

et al. 2020

Cancers

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Background: Cancer-cachexia induces a variety of metabolic disorders, including skeletal muscle imbalance. Alternative therapy, as nutritional supplementation with leucine, shows a modulatory effect over tumour damage in vivo and in vitro. Method: Adult rats distributed into Control (C), Walker tumour-bearing (W), control fed a leucine-rich diet (L), and tumour-bearing fed a leucine-rich diet (WL) groups had the gastrocnemius muscle metabolomic and proteomic assays performed in parallel to in vitro assays. Results: W group presented an affected muscle metabolomic and proteomic profile mainly related to energy generation and carbohydrates catabolic processes, but leucine-supplemented group (WL) recovered the energy production. In vitro assay showed that cell proliferation, mitochondria number and oxygen consumption were higher under leucine effect than the tumour influence. Muscle proteomics results showed that the main affected cell component was mitochondria, leading to an impacted energy generation, including impairment in proteins of the tricarboxylic cycle and carbohydrates catabolic processes, which were modulated and improved by leucine treatment. Conclusion: In summary, we showed a beneficial effect of leucine upon mitochondria, providing information about the muscle glycolytic pathways used by this amino acid, where it can be associated with the preservation of morphometric parameters and consequent protection against the effects of cachexia.

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Section: Discussionsupporting

confidence: 74%