Letrozole and zoledronic acid changed signalling pathways involved in the apoptosis of breast cancer cells

Sheweita, Salah A.; Ammar, Rania G.; Sabra, Sally; Sultan, Ahmed

doi:10.1016/j.jtumed.2020.10.017

Cited by 4 publications

(1 citation statement)

References 33 publications

(21 reference statements)

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“…However, recent evidence has shown that ZA is able to modulate signaling pathways involved in apoptosis and that could be beneficial to be used together with letrozole to treat EsR-positive BC patients (198).…”

Section: Zoledronatementioning

confidence: 99%

Cholesterol and Its Derivatives: Multifaceted Players in Breast Cancer Progression

et al. 2022

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Cholesterol is an essential lipid primarily synthesized in the liver through the mevalonate pathway. Besides being a precursor of steroid hormones, bile acid, and vitamin D, it is an essential structural component of cell membranes, is enriched in membrane lipid rafts, and plays a key role in intracellular signal transduction. The lipid homeostasis is finely regulated end appears to be impaired in several types of tumors, including breast cancer. In this review, we will analyse the multifaceted roles of cholesterol and its derivatives in breast cancer progression. As an example of the bivalent role of cholesterol in the cell membrane of cancer cells, on the one hand, it reduces membrane fluidity, which has been associated with a more aggressive tumor phenotype in terms of cell motility and migration, leading to metastasis formation. On the other hand, it makes the membrane less permeable to small water-soluble molecules that would otherwise freely cross, resulting in a loss of chemotherapeutics permeability. Regarding cholesterol derivatives, a lower vitamin D is associated with an increased risk of breast cancer, while steroid hormones, coupled with the overexpression of their receptors, play a crucial role in breast cancer progression. Despite the role of cholesterol and derivatives molecules in breast cancer development is still controversial, the use of cholesterol targeting drugs like statins and zoledronic acid appears as a challenging promising tool for breast cancer treatment.

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Section: Zoledronatementioning

confidence: 99%

Cholesterol and Its Derivatives: Multifaceted Players in Breast Cancer Progression

et al. 2022

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Targeted Delivery of Letrozole Using a Modified Metal–Organic Framework as a Promising Candidate in Breast Cancer Therapy

Ghaderpour,

Kashanian,

Nazari

et al. 2024

BioNanoSci.

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Evaluation of the Relationship between Aromatase/Sirtuin1 Interaction and miRNA Expression in Human Neuroblastoma Cells

Kartal

Tokat

Kelicen-Uğur

et al. 2023

CMP

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Background: Changes in activation/inhibition of Sirtuin-1 (SIRT1) and aromatase play an important role in a plethora number of diseases. MicroRNAs (miRNAs) modulate multiple molecular pathways and affect a substantial number of physiological and pathological processes Objective: The aim of this study was to investigate any possible interaction between aromatase and SIRT1 in SH-SY5Y cells and to see how there is a connection between this interaction and miRNA expression-if there is an interaction. Methods: In this study, cells were incubated in serum-deprived media for 6, 12, 24 h. Aromatase and SIRT1 expression were evaluated by Western blot. The IC50 concentration of SIRT1 activator (SRT1720), SIRT1 inhibitor (EX527) and aromatase inhibitors (letrozole and fadrozole) was determined by XTT method. Then, CYP19A1 and SIRT1 levels were evaluated in the presence of SIRT1 siRNA or IC50 values for each activator/inhibitor. Finally, CYP19A1, SIRT1 expression and miRNA target gene analyzes were assessed with bioinformatics approaches. Results: Aromatase and SIRT1 protein levels were significantly elevated in the cells incubated at 24 h in serum-deprived media (p ≤ 0.05). SIRT1 also positively regulated CYP19A1 in SH-SY5Y cells in media with/without FBS. Serum deprivation depending on time course caused changes in the oxidant/antioxidant system. While oxidative stress index tends to decrease in the absence of FBS at 24 h compared to the control, it showed a significant decrease in the 48 h serum-deprived manner(p ≤ 0.001). As a result of bioinformatic analysis, we determined 3 miRNAs which could potentially regulate the SIRT1 and CYP19A1. hsa-miR-27a-3p and hsa-miR-181a-5p correlated in terms of their expressions at 24 h compared to 12 h, and there was a significant decrease in the expression of these miRNAs. On the contrary, the expression of hsa-miR-30c-5p significantly increased at 24 h compared to 12 h. Conclusion: When the results are considered altogether, a direct link between aromatase and SIRT1 was present in human neuroblastoma cells. The identification of key miRNAs; hsa-miR-27a-3p, hsa-miR-30c-5p and hsa-miR-181a-5p targeting both aromatase and SIRT1 provided an approach with novel insights in neurology-associated diseases.

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Letrozole and zoledronic acid changed signalling pathways involved in the apoptosis of breast cancer cells

Cited by 4 publications

References 33 publications

Cholesterol and Its Derivatives: Multifaceted Players in Breast Cancer Progression

Cholesterol and Its Derivatives: Multifaceted Players in Breast Cancer Progression

Targeted Delivery of Letrozole Using a Modified Metal–Organic Framework as a Promising Candidate in Breast Cancer Therapy

Evaluation of the Relationship between Aromatase/Sirtuin1 Interaction and miRNA Expression in Human Neuroblastoma Cells

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