Lethal photosensitisation of Staphylococcus aureus using a toluidine blue O–tiopronin–gold nanoparticle conjugate

Gil-Tomás, Jesús J.; Tubby, Sarah; Parkin, Ivan P.; Narband, Naima; Dekker, Linda; Nair, Sean P.; Wilson, Michael; Street, Cale N.

doi:10.1039/b706615e

Cited by 110 publications

(79 citation statements)

References 35 publications

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“…Depending on the properties of this capping agent, the characteristics (geometry and solubility) of the nanoparticles can be controlled. The choice of capping agent must be directed by the characteristics and application of the intended nanoparticles; for biological applications, polypeptides (gluthatione) [21,45,46], tiopronin [10,33] and aminoacids have been previously employed [31,45]. The common feature of these compounds is the presence of sulphur whose strong affinity for Ag results in coverage of the nanoparticles and provides stability during nanoparticles ripening.…”

Section: Discussionmentioning

confidence: 99%

“…citrates [22,28], oleic acid [26], poly(acrylic acid) [29], starch [30] and glutamic acid [31] or prepared with a capping agent that allows binding of other compounds to the nanoparticles such glutathione [32] or tiopronin [33]. Silver nanoparticles of very different geometrical (size and shape) characteristics can be obtained modifying the reaction conditions.…”

Section: Introductionmentioning

confidence: 99%

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Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Perni

Hakala

Prokopovich

2014

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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The number of medical applications of silver nanoparticles is constantly expanding due to their high bactericidal properties coupled with low toxicity towards mammalian cells. Because of this expanding use of silver nanoparticles, novel methods of synthesis have been developed in order to achieve nanoparticles preparation through inexpensive and environmentally friendly processes; biogenic synthesis of silver nanoparticles is an approach that meets those requirements.In this work, E. coli cultures centrigugates were used to synthesis L-cysteine capped silver nanoparticles. The ratio between AgNO 3 and L-cysteine has been proven to affect the size of the nanoparticles: higher amount of L-cysteine returns smaller nanoparticles, but does not affect the percentage of organic matter in the nanoparticles as determined through TGA.The silver nanoparticles prepared had antimicrobial activity against E. coli and S. aureus with Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) independent of the nanoparticles size; for both bacterial species the values of MIC and MBC were the same, 0.0432 mg/ml for E. coli and 0.0216 mg/ml for S. aureus.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Perni

Hakala

Prokopovich

2014

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…36,37 The nanoparticles can also be stabilized using chelating substances such as citrates, 38 oleic acid, 37 and glutamic acid 39 or prepared with a capping agent that allows binding of other compounds to the nanoparticles; for example, glutathione 40 or tiopronin. 41 Silver nanoparticles of very different geometric (size and shape) characteristics can be obtained by modifying the reaction conditions. 42 It is recognized that the preparation method influences the interactions of the silver nanoparticles with living organisms; therefore, the nanoparticles can be tuned to the required application through controlling the synthetic conditions.…”

Section: Introductionmentioning

confidence: 99%

A novel bone cement impregnated with silver–tiopronin nanoparticles: its antimicrobial, cytotoxic, and mechanical properties

Prokopovich¹,

Leech²,

Carmalt³

et al. 2013

IJN

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Post-operatory infections in orthopedic surgeries pose a significant risk. The common approach of using antibiotics, both parenterally or embedded in bone cement (when this is employed during surgery) faces the challenge of the rising population of pathogens exhibiting resistance properties against one or more of these compounds; therefore, novel approaches need to be developed. Silver nanoparticles appear to be an exciting prospect because of their antimicrobial activity and safety at the levels used in medical applications. In this paper, a novel type of silver nanoparticles capped with tiopronin is presented. Two ratios of reagents during synthesis were tested and the effect on the nanoparticles investigated through TEM, TGA, and UV-Vis spectroscopy. Once encapsulated in bone cement, only the nanoparticles with the highest amount of inorganic fraction conferred antimicrobial activity against methicillin resistant Staphylococcus aureus (MRSA) at concentrations as low as 0.1% w/w. No other characteristics of the bone cement, such as cytotoxicity or mechanical properties, were affected by the presence of the nanoparticles. Our work presents a new type of silver nanoparticles and demonstrates that they can be embedded in bone cement to prevent infections once the synthetic conditions are tailored for such applications.

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“…Along with AMPs, several metallic nanoparticles, such as silver (16,17,18), gold (19,20), zinc oxide (21,22), have also emerged as potential alternative for the treatment of drug-resistant bacterial infections. Among them, silver nanoparticles (AgNPs) have attracted great attention in recent years owing to their potent broadspectrum antimicrobial activity, lower propensity to induce microbial resistance than antibiotics, unique mode of action, and ease of generation (23,24).…”

mentioning

confidence: 99%

Cationic Antimicrobial Peptides and Biogenic Silver Nanoparticles Kill Mycobacteria without Eliciting DNA Damage and Cytotoxicity in Mouse Macrophages

Mohanty

Jena

Mehta

et al. 2013

Antimicrob Agents Chemother

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bWith the emergence of multidrug-resistant mycobacterial strains, better therapeutic strategies are required for the successful treatment of the infection. Although antimicrobial peptides (AMPs) and silver nanoparticles (AgNPs) are becoming one of the popular antibacterial agents, their antimycobacterial potential is not fully evaluated. In this study, we synthesized biogenic-silver nanoparticles using bacterial, fungal, and plant biomasses and analyzed their antibacterial activities in combination with AMPs against mycobacteria. Mycobacterium smegmatis was found to be more susceptible to AgNPs compared to M. marinum. We found that NK-2 showed enhanced killing effect with NP-1 and NP-2 biogenic nanoparticles at a 0.5-ppm concentration, whereas LLKKK-18 showed antibacterial activity only with NP-2 at 0.5-ppm dose against M. smegmatis. In case of M. marinum NK-2 did not show any additive activity with NP-1 and NP-2 and LLKKK-18 alone completely inhibited the bacterial growth. Both NP-1 and NP-2 also showed increased killing of M. smegmatis in combination with the antituberculosis drug rifampin. The sizes and shapes of the AgNPs were determined by transmission electron microscopy and dynamic light scattering. AgNPs showed no cytotoxic or DNA damage effects on macrophages at the mycobactericidal dose, whereas treatment with higher doses of AgNPs caused toxicity and micronuclei formation in cytokinesis blocked cells. Macrophages actively endocytosed fluorescein isothiocyanate-labeled AgNPs resulting in nitric oxide independent intracellular killing of M. smegmatis. Apoptosis and cell cycle studies showed that treatment with higher dose of AgNPs arrested macrophages at the G 1 -phase. In summary, our data suggest the combined effect of biogenic-AgNPs and antimicrobial peptides as a promising antimycobacterial template.

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Lethal photosensitisation of Staphylococcus aureus using a toluidine blue O–tiopronin–gold nanoparticle conjugate

Cited by 110 publications

References 35 publications

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

A novel bone cement impregnated with silver–tiopronin nanoparticles: its antimicrobial, cytotoxic, and mechanical properties

Cationic Antimicrobial Peptides and Biogenic Silver Nanoparticles Kill Mycobacteria without Eliciting DNA Damage and Cytotoxicity in Mouse Macrophages

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Lethal photosensitisation of Staphylococcus aureus using a toluidine blue O–tiopronin–gold nanoparticle conjugate

Cited by 110 publications

References 35 publications

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

Biogenic synthesis of antimicrobial silver nanoparticles capped with l-cysteine

A novel bone cement impregnated with silver&ndash;tiopronin nanoparticles: its antimicrobial, cytotoxic, and mechanical properties

Cationic Antimicrobial Peptides and Biogenic Silver Nanoparticles Kill Mycobacteria without Eliciting DNA Damage and Cytotoxicity in Mouse Macrophages

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A novel bone cement impregnated with silver–tiopronin nanoparticles: its antimicrobial, cytotoxic, and mechanical properties