Lethal bronchopneumonia caused by <i>Mycoplasma cynos</i> in a litter of golden retriever puppies

Zeugswetter, Florian K.; Weissenböck, Herbert; Shibly, Sarina; Hassan, Jasmin; Spergser, Joachim

doi:10.1136/vr.161.18.626

Cited by 28 publications

(38 citation statements)

References 12 publications

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“…The role of M. cynos in the pet population has not been fully evaluated, although in the UK, serologic evidence of exposure to M. cynos was found on entry to a shelter and seroconversion increased significantly during housing, particularly in animals with respiratory disease . In a breeding facility, M. cynos was associated with lethal pneumonia in a litter of puppies . Detection of Mycoplasma as the sole isolate in both young and adult dogs here, as in other studies, could support its role as a primary respiratory pathogen, but further investigation is required, and specific identification of mycoplasmal isolates might be needed to assess pathogenicity.…”

Section: Discussionmentioning

confidence: 64%

Microbiologic and Cytologic Assessment of Bronchoalveolar Lavage Fluid from Dogs with Lower Respiratory Tract Infection: 105 Cases (2001–2011)

Johnson¹,

Queen²,

Vernau

et al. 2013

Veterinary Internal Medicne

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Background: Documentation of lower respiratory tract infection has relied on microbiologic and cytologic findings in airway fluid, but there is no gold standard for making a definitive diagnosis.Objective: To report cytologic and microbiologic findings in dogs diagnosed with lower respiratory tract infection through evaluation by bronchoscopy and bronchoalveolar lavage.Animals: A total of 105 dogs with spontaneous respiratory disease. Methods: Retrospective case review of all dogs identified through the electronic medical record database that had bronchoscopy with bronchoalveolar lavage performed between 2001 and 2011. Results of bronchoalveolar lavage cytology and microbiology were evaluated in 510 dogs, and 105 cases with septic, suppurative inflammation or bacterial growth from cultures were examined further.Results: Bacteria were isolated from 89/105 aerobic cultures, 18/104 anaerobic cultures, and 30/99 Mycoplasma spp. cultures. The most common isolate was Mycoplasma spp. followed by Pasteurella sp., Bordetella sp, Enterobacteriaceae, and anaerobes. A single bacterial species was cultured from 44/99 dogs (44%) and multiple bacterial species were isolated from 55/99 dogs (56%). Suppurative inflammation with intracellular bacteria was identified cytologically in 78 of 105 dogs (74%). In 27 dogs that lacked cytologic evidence of sepsis, mixed (n = 18) and neutrophilic (n = 9) inflammation was reported, and Mycoplasma spp. (13/27) or Bordetella spp. (7/27) were most commonly isolated. Most aerobic bacteria were susceptible to routinely used antimicrobial drugs.Conclusions and Clinical Importance: Confirmation of lower respiratory tract infection in dogs is challenging and organisms can be isolated from dogs in which bacteria are not detected on cytologic examination.

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Section: Discussionmentioning

confidence: 64%

Microbiologic and Cytologic Assessment of Bronchoalveolar Lavage Fluid from Dogs with Lower Respiratory Tract Infection: 105 Cases (2001–2011)

Johnson¹,

Queen²,

Vernau

et al. 2013

Veterinary Internal Medicne

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“…Currently, the diagnostic accuracy of cytologic demonstration of B. bronchiseptica organisms in cytologic preparations, such as cytocentrifuged smears of BALF or bronchial brushing fluid (BBF), has not been reported. Moreover, although M. cynos was recently identified as an emerging and possibly lethal pathogen in CIRD, the role of Mycoplasma spp. as primary respiratory pathogens remains unclear .…”

mentioning

confidence: 99%

QuantitativePCRand Cytology of Bronchoalveolar Lavage Fluid in Dogs withBordetella bronchisepticaInfection

Canonne

Billen

Tual

et al. 2016

Veterinary Internal Medicne

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BackgroundThe use of quantitative PCR (qPCR) for detection of Bordetella bronchiseptica in bronchoalveolar lavage fluid (BALF) and demonstration of bacteria adhering to ciliated epithelial cells in BALF or bronchial brushing fluid (BBF) has not been assessed in a series of affected dogs. Coinfections can worsen the clinical severity in bordetellosis, but the specific association with Mycoplasma cynos has not been evaluated.ObjectivesTo assess the utility of culture, qPCR and cytologic examination of cytospin preparations in the diagnosis of bordetellosis in dogs and the influence of coinfection by M. cynos on disease severity.AnimalsTwenty‐four referred dogs with B. bronchiseptica infection and 10 healthy dogs.MethodsRetrospective case series. qPCR (B. bronchiseptica and M. cynos) and culture results from BALF were recorded. Cytospin preparations from BALF and BBF were reviewed. qPCR on BALF from 10 healthy dogs were used as negative control.ResultsThe BALF culture and qPCR detected B. bronchiseptica in 14/24 and 18/18 dogs, respectively. Coccobacilli were found adhering to ciliated epithelial cells in 20 of the 21 BALF cytologic preparations where epithelial cells were found, and 2/3 BBF cytologic preparations. Quantitative PCR detected a low level of B. bronchiseptica in one healthy dog. The frequency of detection of M. cynos was not significantly different in B. bronchiseptica (9/17 dogs) compared with healthy dogs (2/10 dogs) (P = .09).Conclusion and Clinical ImportanceQuantitative PCR detection of B. bronchiseptica in BALF appears to be a useful diagnostic tool. Cytologic examination of BALF or BBF, when positive, allows a rapid and reliable diagnosis.

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“…Rehoming centers and training facilities often suffer endemic CIRD which is difficult to control. Recent evidence suggests that M. cynos in particular is an important etiological agent of CIRD (5–8). To date very little is known about the role M. cynos plays in CIRD, whether the bacterium is a primary pathogen or an opportunistic secondary pathogen.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of Mycoplasma cynos Strain C142

et al. 2013

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Lethal bronchopneumonia caused by Mycoplasma cynos in a litter of golden retriever puppies

Cited by 28 publications

References 12 publications

Microbiologic and Cytologic Assessment of Bronchoalveolar Lavage Fluid from Dogs with Lower Respiratory Tract Infection: 105 Cases (2001–2011)

Microbiologic and Cytologic Assessment of Bronchoalveolar Lavage Fluid from Dogs with Lower Respiratory Tract Infection: 105 Cases (2001–2011)

QuantitativePCRand Cytology of Bronchoalveolar Lavage Fluid in Dogs withBordetella bronchisepticaInfection

Complete Genome Sequence of Mycoplasma cynos Strain C142

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