Let's get small (and smaller): Combining zebrafish and nanomedicine to advance neuroregenerative therapeutics

White, David T.; Saxena, Mohit; Mumm, Jeff S.

doi:10.1016/j.addr.2019.01.011

Cited by 11 publications

(5 citation statements)

References 182 publications

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“…Here the zebrafish is also proving an advantageous model for assessing blood brain barrier permeability with respect to novel neuro-specific technologies. Zebrafish provide an excellent model to test nanoparticle biocompatibility and toxicity ( Chakraborty et al, 2016 ; White et al, 2019 ), and recent studies show success in treatment with nanoparticles to provide neuroprotection from Aβ induced toxicity as seen in AD ( Saleem et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Zebrafish as a model organism for neurodegenerative disease

Chia

Klingseisen

Sieger

et al. 2022

Front. Mol. Neurosci.

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The zebrafish is increasingly recognized as a model organism for translational research into human neuropathology. The zebrafish brain exhibits fundamental resemblance with human neuroanatomical and neurochemical pathways, and hallmarks of human brain pathology such as protein aggregation, neuronal degeneration and activation of glial cells, for example, can be modeled and recapitulated in the fish central nervous system. Genetic manipulation, imaging, and drug screening are areas where zebrafish excel with the ease of introducing mutations and transgenes, the expression of fluorescent markers that can be detected in vivo in the transparent larval stages overtime, and simple treatment of large numbers of fish larvae at once followed by automated screening and imaging. In this review, we summarize how zebrafish have successfully been employed to model human neurodegenerative diseases such as Parkinson’s disease, Alzheimer’s disease, amyotrophic lateral sclerosis, and Huntington’s disease. We discuss advantages and disadvantages of choosing zebrafish as a model for these neurodegenerative conditions.

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Section: Discussionmentioning

confidence: 99%

Zebrafish as a model organism for neurodegenerative disease

Chia

Klingseisen

Sieger

et al. 2022

Front. Mol. Neurosci.

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“…The Danio rerio (zebrafish) is a demonstrated vertebrate model for exploring development studies and the study of degenerative diseases [ 49 – 52 ]. It can be modelled for far ranging analyses, from fundamental and toxicological analysis to pre-clinical studies [ 53 – 55 ]. Of the several advantages offered by the zebrafish, its cost-effective maintenance, easy testing with simple housing requirements and a large clutch size are highly suited for high throughput testing [ 56 ].…”

Section: Zebrafish As a Model For Neurospecific Drug Deliverymentioning

confidence: 99%

Zebrafish: A Promising Real-Time Model System for Nanotechnology-Mediated Neurospecific Drug Delivery

Saleem

Kannan

2021

Nanoscale Res Lett

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Delivering drugs to the brain has always remained a challenge for the research community and physicians. The blood–brain barrier (BBB) acts as a major hurdle for delivering drugs to specific parts of the brain and the central nervous system. It is physiologically comprised of complex network of capillaries to protect the brain from any invasive agents or foreign particles. Therefore, there is an absolute need for understanding of the BBB for successful therapeutic interventions. Recent research indicates the strong emergence of zebrafish as a model for assessing the permeability of the BBB, which is highly conserved in its structure and function between the zebrafish and mammals. The zebrafish model system offers a plethora of advantages including easy maintenance, high fecundity and transparency of embryos and larvae. Therefore, it has the potential to be developed as a model for analysing and elucidating the permeability of BBB to novel permeation technologies with neurospecificity. Nanotechnology has now become a focus area within the industrial and research community for delivering drugs to the brain. Nanoparticles are being developed with increased efficiency and accuracy for overcoming the BBB and delivering neurospecific drugs to the brain. The zebrafish stands as an excellent model system to assess nanoparticle biocompatibility and toxicity. Hence, the zebrafish model is indispensable for the discovery or development of novel technologies for neurospecific drug delivery and potential therapies for brain diseases.

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“…As a stable and accessible evaluation model, zebrafish models have the advantages of simplicity, efficiency and visualization, which can commendably bridge the gap between in vitro cell models and in vivo mammalian models [ 15 , 16 ]. The zebrafish imaging can achieve nearly real-time pharmacokinetic research, allowing researchers to directly observe the dynamic changes in fluorescent drugs in vivo [ 17 ]. In addition, it can be stanchly combined with high-throughput screening technology to evaluate the absorption, distribution, metabolism and excretion of drugs under different conditions [ 18 ], which provides a real-time and visual platform for pharmacokinetic research.…”

Section: Introductionmentioning

confidence: 99%

Nobiletin with AIEE Characteristics for Targeting Mitochondria and Real-Time Dynamic Tracking in Zebrafish

Jin

et al. 2023

Molecules

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Nobiletin is a natural product with multiple physiological activities and is the main ingredient of Pericarpium Citri Reticulatae. We successfully discovered that nobiletin exhibits aggregation induced emission enhancement (AIEE) properties and it has significant advantages such as a large Stokes shift, good stability and excellent biocompatibility. The increase in methoxy groups endows nobiletin a greater fat-solubility, bioavailability and transport rate than the corresponding unmethoxylated flavones. Ulteriorly, cells and zebrafish were used to explore the application of nobiletin in biological imaging. It emits fluorescence in cells and is specifically targeted at mitochondria. Moreover, it has a noteworthy affinity for the digestive system and liver of zebrafish. Due to the unique AIEE phenomenon and stable optical properties of nobiletin, it paves the way for discovering, modifying and synthesizing more molecules with AIEE characteristics. Furthermore, it has a great prospect with regard to imaging cells and cellular substructures, such as mitochondria, which play crucial roles in cell metabolism and death. Indeed, three-dimensional real-time imaging in zebrafish provides a dynamic and visual tool for studying the absorption, distribution, metabolism and excretion of drugs. In this article, more directions and inspiration can be presented for the exploration of non-invasive pharmacokinetic research and intuitive drug pathways or mechanisms.

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Let's get small (and smaller): Combining zebrafish and nanomedicine to advance neuroregenerative therapeutics

Cited by 11 publications

References 182 publications

Zebrafish as a model organism for neurodegenerative disease

Zebrafish as a model organism for neurodegenerative disease

Zebrafish: A Promising Real-Time Model System for Nanotechnology-Mediated Neurospecific Drug Delivery

Nobiletin with AIEE Characteristics for Targeting Mitochondria and Real-Time Dynamic Tracking in Zebrafish

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