Lessons from the history of light microscopy

Amos, Brad

doi:10.1038/35019639

Cited by 44 publications

(28 citation statements)

References 11 publications

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“…For centuries, the development of optical microscopes has revolutionized fundamental life science and clinical practice . By magnifying minuscule cellular and subcellular features, optical microscopes provide a powerful tool for studying tissue components and their dynamic interactions.…”

Section: Introductionmentioning

confidence: 99%

Photoacoustic microscopy

Yao

Wang

2013

Laser & Photonics Reviews

428

418

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Photoacoustic microscopy (PAM) is a hybrid in vivo imaging technique that acoustically detects optical contrast via the photoacoustic effect. Unlike pure optical microscopic techniques, PAM takes advantage of the weak acoustic scattering in tissue and thus breaks through the optical diffusion limit (~1 mm in soft tissue). With its excellent scalability, PAM can provide high-resolution images at desired maximum imaging depths up to a few millimeters. Compared with backscattering-based confocal microscopy and optical coherence tomography, PAM provides absorption contrast instead of scattering contrast. Furthermore, PAM can image more molecules, endogenous or exogenous, at their absorbing wavelengths than fluorescence-based methods, such as wide-field, confocal, and multi-photon microscopy. Most importantly, PAM can simultaneously image anatomical, functional, molecular, flow dynamic and metabolic contrasts in vivo. Focusing on state-of-the-art developments in PAM, this Review discusses the key features of PAM implementations and their applications in biomedical studies.

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Section: Introductionmentioning

confidence: 99%

Photoacoustic microscopy

Yao

Wang

2013

Laser & Photonics Reviews

428

418

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“…Optical microscopy has evolved to three dimensional and four-dimensional image formation and analysis tool and offered a means to probe various processes at submicron and nanometer levels [6,7]. Here we mainly introduced the techniques and applications frequently used in human LCI.…”

Section: The Basic Principle Of Lcimentioning

confidence: 99%

Application of Probes in Live Cell Imaging~!2010-01-03~!2010-05-09~!2010-08-16~!

Chen¹,

Bai²,

Wang³

2010

JEBP

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Live cell imaging (LCI) is the approach of non-invasively analyzing dynamic processes in living cells using state-of-the-art microscopy and computer vision techniques. LCI provides exciting and novel insights into cell biology. The present review summarized LCI research in recent years and detailed the role of probes in LCI. We discussed the basic principle and development in LCI technology, including fluorecence resonance energy transfer, fluorescence lifetime imaging microscopy, atomic force microscopy, scanning ion conductance microscopy, confocal microscopy, two-photon excitation fluorescence microscopy and Cell-IQ® System . Then we listed several probes such as green fluorescent protein and quantum dots which are the most widely used probes. Proteins, organelles, nucleotides and other chemicals related to cell biology may all be potential targets. We also discussed the advantages and disadvantages of different probes. Although specific probes in LCI still need to be explored, the application of LCI may be attractive for better understanding of cellular functions.

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“…While the 16 th century remained relatively quiet in terms of the advancement of neuroscience there was one important work developing in the workshop of two Dutch eye glass makers that would prove to be pivotal for the advancement of cell biology, including our understanding of brain structure and function. Zaccharias Janssen and his father Hans engineered the first rudimentary microscope using tubes and two lenses (Amos, 2000). Though their microscope was not used extensively for research purposes it was a critical step towards development of the modern microscope.…”

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confidence: 99%

Neurogenin1 Expression and Function in the Developing Mouse Cerebellum

Lundell¹

2011

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The basic helix-loop-helix (bHLH) transcription factors Ptf1a and Math1 are necessary for the specification of gamma-aminobutyric acid (GABA)-ergic and glutamatergic cell lineages in the cerebellum, respectively. Recent evidence suggests that cascades of bHLH factor activities drive cell type specificity in Ptf1a-positive and Math1positive lineages. Neurogenin1 (Neurog1) is a bHLH transcription factor involved in the development of several nervous system structures including the cerebral cortex, trigeminal sensory neurons, inner-ear sensory neurons, and olfactory sensory neurons.Recently, expression of Neurog1 has been reported to be co-expressed in Ptf1aexpressing cells in the ventricular zone (VZ) of the developing cerebellum, suggesting a role in subtype specification of GABAergic progenitor cells.Using immunohistochemistry and transgenic reporter mice, we have demonstrated that Neurog1 is expressed during a prolonged period of cerebellar development that spans embryonic and postnatal phases of neurogenesis. Neurog1 is expressed in neuronal cell lineages of the cerebellar cortex, primarily by GABAergic cell lineages. However, Neurog1 is also expressed in small populations of glutamatergic unipolar brush cells and by a very limited number of granule cells.Using inducible reporter mice, we have determined the temporal sequence of Neurog1-fated progenitors. Neurog1-fated progenitors first specify Purkinje cells in the xii DEDICATIONOne of life's greatest gifts is the opportunity to freely pursue personal ambitions and find satisfaction in the achievement of them. I feel privileged to have had this opportunity to earn an advanced degree in neuroscience while exploring the mysteries of the brain and its development. My gratitude is deepened and my resolve to make the most out of life strengthened when I reflect upon the shortened lives of several fellow classmates from my hometown of Spanish Fork, Utah. Although they did not live long enough to fully persue their hopes and dreams their memory remains in those who knew them. And if nothing else that memory inspires us to seize every moment. This dissertation is dedicated to them, their parents, and family members.

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Lessons from the history of light microscopy

Cited by 44 publications

References 11 publications

Photoacoustic microscopy

Photoacoustic microscopy

Application of Probes in Live Cell Imaging~!2010-01-03~!2010-05-09~!2010-08-16~!

Neurogenin1 Expression and Function in the Developing Mouse Cerebellum

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